Efficient in vitro multiplication in Ornithogalum ulophyllum Hand.-Mazz. from twin scale explants

Özel, Çiğdem Alev; Khawar, Khalid Mahmood; Erkul, Seher Karaman; Ateş, Mevlüde Alev; Arslan, Orhan

doi:10.1016/j.scienta.2007.11.006

Cited by 14 publications

(7 citation statements)

References 10 publications

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“…Other studies on Indigofera, by Rajabudeen et al (2014) notified some of shoots of I. viscosa were rooted on the medium supplemented with 2 mg/L BAP and 2.5 m/L NAA. Howell et al (1986), Ozel et al (2008Ozel et al ( , 2015 reported that I. potaninii, Ornithogalum ulophyllum and Muscari muscarimi easily regenerate shoots and bulbs on medium containing high level of both BAP and NAA. The above-mentioned rooting results are fully or partially in agreement with previous studies.…”

Section: Discussionmentioning

confidence: 99%

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Özel¹,

Maesaroh²

2018

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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Indigofera zollingeriana is an important protein rich forage and dye plant species that grows widely in Indonesia. It is difficult to propagate the plant through seeds due to high dormancy. Therefore, there is need to develop both in vitro and ex vitro propagation techniques of I. zollingeriana. This study aimed to develop a protocol for multiple shoot regeneration of the plant under in vitro conditions using different combinations of BAP+0.01 mg/L NAA. The results showed that both epigeal and cotyledon node explants have high potential to regenerate with maximum number of regenerated shoots noted on the epigeal and the cotyledon node explants using MS medium containing 1.5 mg/L BAP + 0.01 mg/L of NAA and 1.0 mg/L BAP + 0.01 mg/L of NAA in the same order. The shoots regenerated on all combinations of BAP+NAA were rooted using 0.5 mg/L IBA that promoted rooting in effective manner. The rooted plants were easily acclimatized to external conditions in an environmental chamber. The results suggest that I. zollengriana plant species is no more difficult to regenerate under in vitro conditions. This research will aid in future studies related to breeding, propagation, biosynthesis and extraction of active compounds.

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Section: Discussionmentioning

confidence: 99%

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Özel¹,

Maesaroh²

2018

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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“…This study is of enormous importance for regeneration of L. candidum making large-scale micropropagation feasible. Real issues of tissue culture plants is their encounter of transplantation shock (Ozel et al, 2008b) that could be avoided significantly by covering…”

Section: Acclimatizationmentioning

confidence: 99%

Efficient Approaches to in vitro Multiplication of Lilium candidum L. with Consistent and Safe Access throughout Year and Acclimatization of Plant under Hot-Summer Mediterranean (Csa Type) Climate

Royandazagh

2019

Not Bot Horti Agrobo

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Lilium candidum L. is one of the most fragrant plant species among Liliaceae. The indigenous and natural populations of L. candidum are significantly affected by fast increasing anthropological population pressure and increased carbon fuel pollution. There is a necessity for development of a micropropagation protocol to serve in effective manner for both commercial multiplication and protection of the plant. The study targeted to develop a strategy for effective in vitro plant propagation system using static liquid culture, seismomorphogenic treatments with mixed liquid culture and semi solid culture medium using single scale explants. The former two techniques failed to regenerate new bulblets effectively whereby the later technique induced 100% regeneration on all treatments. Maximum number of bulblets regenerated on 0.27 µM Thidiazuron + 1.08 µM Naphthalene acetic acid. The scale explant could be recultured and regenerated on the mother culture medium for six cycles. The daughter bulblets regenerated on Murashige and Skoog medium containing both 0.18 or 0.27 µM TDZ + 1.08 µM NAA were successfully rooted using 1/2 × MS medium containing 2.45 µM indole butyric acid. Regardless of rooting development, acclimatization was noted on all bulblets in the greenhouse. These plants are showing continuous flowering since last four years under hot-summer Mediterranean (CSa type) climate field conditions of Tekirdag province of Thrace region in Turkey. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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“…(Chan et al 2009); Hoslundia opposita Vahl. (Prakash and Van Staden 2007), Hypericum perforatum L. (Danova et al 2012;Savio et al 2012), Labisia pumila (Hartinie and Jualang 2007), Leptadenia reticulate (Kalidass et al 2008), Mollugo nudicaulis (Nagesh and Shanthamma 2011), Ornithogalum ulophyllum (Ozel et al 2008), Ocimum gratissimum L (Gopi et al 2006 A good example of such studies is on Rhodiola rosea that is an endangered medicinal species with a limited distribution. It has an outstanding importance for the pharmaceutical industry, in the prevention and cure of cancer, heart and nervous system diseases etc.…”

Section: Propagation By Shoot and Node Culturementioning

confidence: 99%

In Vitro Micropropagation of Medicinal and Aromatic Plants

Máthé

Hassan

Kader

2015

Medicinal and Aromatic Plants of the World

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Modern biotechnological methods like in vitro micropropagation technique hold tremendous potential for the production of high-quality plant-based medicine. They also allow to achieve the large scale multiplication of diseasefree plants, faster cloning and the conservation of desired genotypes, in a very short span of time. Via genetic transformation techniques, the modification of both genetic information of MAPs and the regulation of genes responsible for the production of valuable biologically active substances has also become possible in either higher amounts or with better properties.Micropropagation protocols are worked out for many plant species cultured in vitro to provide macro-and micro-mineral nutrients, vitamins, source of carbohydrates, appropriate environmental conditions (light intensity, photoperiod and temperature) and plant growth regulators required to obtain high regeneration rates. As such they are expected to facilitate commercially feasible micropropagation. Well-defined cell culture methods have also been developed to produce pharmacologically important secondary metabolites. Genetic engineering is applied to produce transgenic medicinal plants and metabolites.This chapter offers a brief insight into the present status of the biotechnology of MAPs. Special emphasis has been placed on the in vitro micropropagation and rapid clonal multiplication of selected elite genotypes, the regulation of organogenesis and somatic embryogenesis, the exploitation of advantages in somaclonal variation and genetic engineering techniques for both crop improvement and in vitro germplasm conservation. The production of flavor and volatile constituents in tissue cultures will also be briefly discussed, similarly to the application of farther biotechnological approaches.

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Efficient in vitro multiplication in Ornithogalum ulophyllum Hand.-Mazz. from twin scale explants

Cited by 14 publications

References 10 publications

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Efficient Approaches to in vitro Multiplication of Lilium candidum L. with Consistent and Safe Access throughout Year and Acclimatization of Plant under Hot-Summer Mediterranean (Csa Type) Climate

In Vitro Micropropagation of Medicinal and Aromatic Plants

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