Efficient cutaneous wound healing using bixin-loaded PCL nanofibers in diabetic mice

Abstract

The present work demonstrated an efficient cutaneous wound healing using Bixin-loaded polycaprolactone (PCL) nanofibers as a controlled delivery system. The influence of Bixin (Bix) content on PCL nanofiber, Bix-PCL1(2.5% w/w bix) and Bix-PCL2 (12.5% w/w bix) formation was investigated using electrical conductivity, attenuated total reflectance infrared spectroscopy, X-ray diffraction, thermal analysis, and scanning electronic microscopy. The results showed that a greater bixin concentration resulted in higher polymeric solution electrical conductivity. Moreover, higher polymeric solution electrical conductivity provides lower nanofibers in terms of average diameter than pure PCL nanofibers. In vitro release was largely governed by a diffusion-controlled mechanism. The initial Bixin release domain showed a burst release over the first 10 hours where approximately 30% and 40% of Bixin was released from Bix-PCL1 and Bix-PCL2 nanofibers, respectively. The second kinetic domain was comprised of a continuous and slow Bixin release that led to almost 100% of the Bixin being released within 14 days. The results on excisional wound model in induced diabetic mice indicated that the low concentration of Bixin released from loaded Bix-PCL nanofibers maintain the biological activity of Bixin and is efficient in accelerating the wound healing as well as in reducing the scar tissue area compared with pure PCL nanofibers. Therefore, soft material Bixin-loaded PCL nanofibers are a promising candidate for use in wound dressing. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1938-1949, 2017.

Plant-Based Antidiabetic Nanoformulations: The Emerging Paradigm for Effective Therapy

“…Bixin-loaded PCL nanofibers were found to be a biocompatible formulation and exhibited an initial burst release (30%-40%, in the first 10 h) followed by a sustained release (100% in day 14) of bixin. Bixin-PCL nanofibers exhibited the excellent ability of tissue regeneration and wound repair to promote the healing of diabetic wounds in mice [324]. Lutein, a xanthophyll carotenoid, has been reported to be useful in preventing hyperglycaemia-induced oxidative stress, inflammation, and cataract development without affecting glycaemic status [325,326].…”

Electrospun nanofibrous materials for wound healing applications

“…The overall results indicated that the incorporation of ZnO nanoparticles notably enhanced the wound healing effects. And also, in another recent study reported by Pinzón-García et al [45], Bixin (Bix), an antioxidant carotenoid pigment, was loaded to PCL nanofibers, and the in vivo wound healing study in diabetic mice resulted in accelerated wound healing and in reduced scar formation as compared with pure PCL nanofibers.…”

Functional Nanofibrous Biomaterials of Tailored Structures for Drug Delivery—A Critical Review

“…Polycaprolactone (PCL) has been widely explored due to its excellent properties (biocompatibility, biodegradation, non-toxicity, low melting point (60 • C) and semi-crystallinity) and low cost. In addition, PCL can be dissolved into many common solvents, such as HFIP, chloroform, acetic acid, methanol and dichloromethane [37,82,83]. Due to these advantages, PCL is frequently utilized to produce multi-functional nanofibers for drug delivery in tissue engineering applications.…”