Efficient callus induction and plant regeneration from filaments with anther in lily (Lilium longiflorum Thunb.)

Fernández, Amaury Martín Arzate; Nakazaki, Tetsuya; Okumoto, Yutaka; Tanisaka, Takatoshi

doi:10.1007/s002990050330

Cited by 32 publications

(12 citation statements)

References 16 publications

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“…B-5 and N-6 media were both superior to MS medium for callus production from fi laments with anthers in Lilium longiflorum Thunb. (Arzate-Fernandez et al, 1997). The sucrose concentration utilized in that study was 9%.…”

Section: Resultsmentioning

confidence: 99%

Basal Medium and Sucrose Concentration Influence Regeneration of Easter Lily in Ovary Culture

Ramsay¹,

Galitz²,

Lee³

2003

HortSci

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Influences of culture media and sucrose concentrations on plant regeneration from Easter lily (Lilium longiflorum L. cv. Ace) ovary tissues were investigated. Pistils excised from unopened flower buds (3-5 cm long) were sectioned and cultured on either B-5 medium or Murashige and Skoog (MS) medium containing 2%, 5%, or 10% sucrose, with 1 mg·L-1 2,4-D and 2 mg·L-1 BA. Callus formation was most prolific on MS medium containing 5% sucrose. Shoot differentiation was higher on MS medium than on B-5 medium. Rooted plants were transferred into soil medium and grown in a greenhouse. Root tip smears showed that 35% of the regenerated plants showed a variation in chromosome numbers from 10 to 25 per cell, while the rest of the regenerants showed the normal 2n = 2x = 24 chromosomes per cell. The mixoploid condition also existed in different root cells of the same regenerated plant. Chemical names used: 2,4-dichlorophenoxyacetic acid (2,4-D); 6-benzylaminopurine (BA).

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Section: Resultsmentioning

confidence: 99%

Basal Medium and Sucrose Concentration Influence Regeneration of Easter Lily in Ovary Culture

Ramsay¹,

Galitz²,

Lee³

2003

HortSci

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“…Although in vitro bulblet induction has been reported in several plant species, the number of bulblets regenerated per explant was low in most cases (Arzate-Fernandez et al 1997;Nayak et al 1997;Ulrich et al 1999;Ziv and LilienKipnis 2000;Paek and Murthy 2002). In an earlier work on bulblet regeneration in C. orchioides, a maximum number of 10 bulblets per explant was reported (Suri et al 1999).…”

Section: Resultsmentioning

confidence: 96%

High-frequency, direct bulblet induction from rhizome explants of Curculigo orchioides Gaertn., an endangered medicinal herb

Thomas

2007

In Vitro Cell.Dev.Biol.-Plant

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An efficient and reproducible method for inducing a large number of bulblets from rhizome explants of Curculigo orchioides Gaertn., an endangered medicinal herb, has been developed. The rhizome pieces, measuring about 1 × 1 cm (length × width), were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of the cytokinins 6-benzylaminopurine, kinetin, and thidiazuron (TDZ) alone or in combination with 1-naphthalene acetic acid or indole-3-butyric acid (IBA). Of the three cytokinins used, TDZ at 7 μM gave the maximum response, with 82% of the cultures responding with an average number of 15.4 bulblets per explant. The addition of auxins with cytokinin considerably increased the response. The optimum induction occurred on MS medium supplemented with 7 μM TDZ and 0.5 μM IBA. On this medium, 88% of the cultures responded with an average number of 21.4 bulblets per explant. Experiments were also carried out to investigate the role of the sugars sucrose, mannose, and glucose along with 7 μM TDZ and 0.5 μM IBA. The results indicate that sucrose and mannose at particular concentrations have critical roles in promoting in vitro bulblet induction. The maximum result was observed on MS medium supplemented with 7 μM TDZ, 0.5 μM IBA, and 200 mM mannose. On this medium, 97% of the cultures responded with an average number of 26.8 bulblets per culture. Several secondary bulblets developing from the leaf blades of primary bulblets were produced when the latter were transferred to MS basal medium for further development. Out of the 45 bulblets transferred to soil, 40 survived. This protocol can be used for the rapid micropropagation of this endangered medicinal herb.

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“…Successful bulblet induction on bulbscales-raised callus in L. regale is reported (Pelkonen 2005). Besides this, callus raised on filaments with anther is also reported to regenerate lily (L. longiflorum) bulblets (Arzate-Fernandez et al 1997).…”

Section: Bulblet Regenerationmentioning

confidence: 97%

In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya

Joshi

Dhar

2009

Acta Physiol Plant

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In vitro propagation protocol for Lilium oxypetalum, a high altitude Himalayan endemic lily, has been developed. Effect of explant types (i.e., callus and in vitro bulblet scales) and sucrose concentration [0-6.0% (w/ v)] on in vitro bulblet regeneration of L. oxypetalum was tested in previously optimized Murashige and Skoog basal medium supplemented with 2.0 lM 6-benzyladenine and 0.1 lM a-naphthaleneacetic acid. Callus explants produced significantly (P \ 0.01) higher number of bulblets per explant than bulblet scale explants. Of the different concentrations of sucrose tested, 4.5% (w/v) sucrose showed significantly (P \ 0.01) higher percentage regeneration (i.e., 70.8 ± 4.2 and 79.2 ± 4.2% regeneration on callus and bulblet scale explants, respectively), and produced higher number of bulblets per explant (i.e., 9.0 ± 0.4 and 5.4 ± 0.5 bulblets on callus and bulblet scale explants, respectively). Regenerated bulblets developed 2-3 leaves when subcultured for 4 weeks and were subsequently transferred ex vitro with a survival rate of 66.7% after 6 weeks. Leaves of the survived plantlets became dry after growing ex vitro for 10 weeks, amongst which 86.4% re-sprouted after remaining dormant for 5-6 weeks and produced 1.5 bulblets per explant. Findings of the present study hold promise for efficiently multiplying the target species in view of its potential economic and conservation significance.

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Efficient callus induction and plant regeneration from filaments with anther in lily (Lilium longiflorum Thunb.)

Cited by 32 publications

References 16 publications

Basal Medium and Sucrose Concentration Influence Regeneration of Easter Lily in Ovary Culture

Basal Medium and Sucrose Concentration Influence Regeneration of Easter Lily in Ovary Culture

High-frequency, direct bulblet induction from rhizome explants of Curculigo orchioides Gaertn., an endangered medicinal herb

In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya

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