Basal Medium and Sucrose Concentration Influence Regeneration of Easter Lily in Ovary Culture

Abstract

<jats:p>Influences of culture media and sucrose concentrations on plant regeneration from Easter lily (<jats:italic>Lilium longifloru</jats:italic>m L. cv. Ace) ovary tissues were investigated. Pistils excised from unopened flower buds (3-5 cm long) were sectioned and cultured on either B-5 medium or Murashige and Skoog (MS) medium containing 2%, 5%, or 10% sucrose, with 1 mg·L<jats:sup>-1</jats:sup> 2,4-D and 2 mg·L<jats:sup>-1</jats:sup> BA. Callus formation was most prolific on MS medium containing 5% sucrose. Shoot differentiation was higher on MS medium than on B-5 medium. Rooted plants were transferred into soil medium and grown in a greenhouse. Root tip smears showed that 35% of the regenerated plants showed a variation in chromosome numbers from 10 to 25 per cell, while the rest of the regenerants showed the normal 2n = 2x = 24 chromosomes per cell. The mixoploid condition also existed in different root cells of the same regenerated plant. Chemical names used: 2,4-dichlorophenoxyacetic acid (2,4-D); 6-benzylaminopurine (BA).</jats:p>

Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia

“…A good response was also observed on several of the media where the auxin:cytokinin ratio was 1:1, such as 50 lmol L -1 each of 2,4-D and BA. These results were somewhat consistent with the observations of Boltenkov et al (2004) and Ramsey (2003) who found with Iris and Lilium, respectively, that the combination of 2,4-D and BA was effective at similar auxin : cytokin ratios of 1:1 or 2:1. More work needs to be conducted to optimise the long-term culture of D. excelsa callus, which has so far proven to be slow.…”

“…Micropropagation techniques have also been widely used with success on a range of related monocotyledons such as those of the Iridaceae (Boltenkov et al 2004;Boltenkov and Zarembo 2005;Shibli and Ajlouni 2000;Shimizu et al 1997) and Liliaceae (Griesbach 1989;Ramsey et al 2003) that now have wide commercial value.…”

Somatic Embryogenesis: Fundamental Aspects and Applications

“…It has been reported that concentrations of carbohydrates stimulate histodifferentiation (Ramsay et al 2003). In A. vera-cruz (Tejavathi et al 2007) and in A.…”

Lilium candidum L.’nin MİKROÇOĞALTIMINDA BAZI ANTİBİYOTİK ve FUNGUSİT UYGULAMALARININ ETKİSİ

“…There are a lot of studies which have been investigated about Lilium species propagated from scale by micropropagation and also in vitro regeneration from scales of Lilium [7][8][9][10][11]. These studies associated with plant growth regulators -such as auxin and cytokinin concentrations- [3,4,7,[12][13][14][15][16], light application [11,[17][18][19][20], scale position and explant size [7,12,17,21,22], sucrose concentration [7,11,12,[22][23][24]. There are no studies on micropropagation of L. candidum about effect of some antibiotic and fungicide applications.…”