In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya

Joshi, Soumai Kant; Dhar, Uppeandra

doi:10.1007/s11738-009-0299-y

Cited by 12 publications

(11 citation statements)

References 22 publications

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“…In vitro adventitious bud regeneration from scales of Lilium rely on many factors like cytokinin and auxin concentrations such as BA and NAA and sucrose concentration (Varshney et al 2000;Kumar et al 2005). Many previous studies found that bulblet growth was favorable when using a high concentration of sucrose in the growth medium (Joshi and Dhar 2009;Bakhshaie et al 2010).…”

Section: Introductionmentioning

confidence: 99%

In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv. “Starfighter” plants

et al. 2019

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Background: Lily is one of the most important commercial cut flower species and one of the three major bulb crops in the commercial market. The aim was to assess and establish a suitable protocol for increasing the number of Lilium bulblets and enlarge their size. Methodology: Different plant growth regulators (TDZ, 2,4-D, 2ip, and picloram) at different concentrations for direct and indirect micropropagation were used. Also, the effect of various concentrations of sucrose alone or with growth inhibitor on bulblet formation during three repeated subcultures was examined with anatomical study. Results: For direct shoot organogenesis, culturing leaf explant on MS culture medium containing TDZ (0.5 mg/l) + 2,4-D (10 mg/l) led to the highest number of shootlets after 1 month. For indirect micropropagation, MS was supplemented with 2ip (0.5 mg/l) + picloram (5 mg/l)-induced callus tissues which were differentiated to embryos then weak shoots after 3 months. For more proliferation, the highest numbers of shootlets, leaves, and bulb scales were observed on MS culture medium supplemented with 1.0 mg/l of BA plus 0.2 mg/l NAA. Using 60 g/l of sucrose plus 3 or 6 mg/l of paclobutrazol led to the highest number of bulblets/explant for the third subculture. The highest bulblet diameters were recorded with 120 g/l sucrose plus 6 or 3 mg/l paclobutrazol. Conclusion: This study had reached to optimize a suitable protocol for direct and indirect micropropagation of Lilium orientalis cv. "Starfighter" bulbs and increase the number and size of bulbs that can be beneficial for increasing the production and reduce their price. This study will help the producers for commercial purposes.

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Section: Introductionmentioning

confidence: 99%

In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv. “Starfighter” plants

et al. 2019

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“…Generally 1/2 to full concentration of salt were used and 30 gl -1 sucrose was added to culture medium for shoot proliferation (Faisal et al 2006). In a procedure for rooting of shoots, reduced concentration of medium salt and sucrose were used (Khan et al 1999;Singh et al 2008) However, in bulbous plants, high concentration of culture medium salt and sucrose was oftenly applied to produce more shoots and increase bulblet growth (Takayama and Misawa 1983;Bonnier and Van Tuyl 1997;Hu et al 2006;Joshi and Dhar 2009). In this study, shoot number and fresh weight per explant had increased significantly on medium containing double strength of LS salt and 50 gl -1 sucrose.…”

Section: Resultsmentioning

confidence: 99%

In vitro micropropagation of water hyacinth (Eichhornia crassipes)

Suh¹,

Park²,

Han³

2010

Journal of Plant Biotechnology

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This study was conducted to refine a micropropagation method of water hyacinth (Eichhornia crassipes) in vitro. When young shoots were cultured on media with various concentrations of BA or TDZ alone, LS medium containing 5.0 mgl-1 BA was found favorable for shoot proliferation from young shoots with a mean of 4.2 shoots. Using BA together with IAA, more shoots were obtained on LS medium containing 5.0 mgl-1 BA and 1.0 mgl-1 IAA with a mean of 5.7 shoots. In liquid medium, number of shoots and fresh weight per explant increased significantly. The best shoot proliferation and increasing of fresh weight were achieved on LS liquid medium containing 5.0 mgl-1 BA and 1.0 mgl-1 IAA with 6.9 shoots and more than 4,000 mg fresh weight. Of the different concentrations of LS salt, double strength of LS medium provided the highest shoot proliferation with 7.3 shoots, and fresh weight with 5,539 mg per explant. Shoot proliferation on LS medium containing 50 gl-1 sucrose had better results with 8.7 shoots and 5,979 mg per explant in fresh weight than other conditions. In conclusion, the optimal level for shoot proliferation and biomass increase of water hyacinth was attained with the application of the double strength of LS medium containing 5.0 mgl-1 BA, 1.0 mgl-1 IAA and 50 gl-1 sucrose.

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“…The formation and development of bulblets are crucial in the life cycle . Despite lots of literature on micropropagation of Lilium (Joshi and Dhar, 2009;Saadon and Zaccai, 2013), the physiological and biochemical mechanisms governing bulblet development remain unclear. Much evidence has shown that HA favors plant growth in many ways but is limitedly used in bulbous crops including in vitro.…”

Section: Discussionmentioning

confidence: 99%

Low humic acids promote in vitro lily bulblet enlargement by enhancing roots growth and carbohydrate metabolism

Xia

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid (HA) on bulblet swelling and the carbohydrate metabolic pathway in Lilium Oriental Hybrids 'Sorbonne' under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations (≥2.0 mg/L) within 45 d after transplanting (DAT). The activities of three major starch synthetic enzymes (including adenosine 5'-diphosphate glucose pyrophosphorylase, granule-bound starch synthase, and soluble starch synthase) were enhanced dramatically after HA application especially low concentration HA (LHA), indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm, respectively. The fresh bulblet weight and diameter reached 468 mg (2.9 times that under the control treatment) and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.

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In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya

Cited by 12 publications

References 22 publications

In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv. “Starfighter” plants

In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv. “Starfighter” plants

In vitro micropropagation of water hyacinth (Eichhornia crassipes)

Low humic acids promote in vitro lily bulblet enlargement by enhancing roots growth and carbohydrate metabolism

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