Efficiency of Treatments Involving Ultraviolet Irradiation for Decontaminating Packaging Board of Different Surface Compositions

Stannard, Catherine J.; Abbiss, John S.; Wood, John M.

doi:10.4315/0362-028x-48.9.786

Cited by 15 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies have investigated the use of PL to inactive microorganisms on solid substrates (Dunn et al, 1997;Haughton et al, 2011;McDonald et al, 2000;Stannard et al, 1985Stannard et al, , 1983Turtoi and Nicolau, 2007;Uesugi et al, 2007;Warriner et al, 2000). It has been hypothesized that physical properties of surfaces including surface topography and reflectivity affect the efficacy of PL inactivation.…”

Section: Introductionmentioning

confidence: 98%

Pulsed Ligh inactivation of Listeria innocua on food packaging materials of different surface roughness and reflectivity

Ringus

Moraru

2013

Journal of Food Engineering

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 98%

Pulsed Ligh inactivation of Listeria innocua on food packaging materials of different surface roughness and reflectivity

Ringus

Moraru

2013

Journal of Food Engineering

View full text Add to dashboard Cite

“…UV has several deleterious eVects on the cellular level such as DNA base damage, strand breaks, protein-DNA cross-links, membrane peroxidation, and enzyme inactivation (reviewed in Sinha and Häder 2002;Cadet et al 2005;Goosen and Moolenaar 2008). UV-C irradiation is a convenient germicidal agent and used for disinfection purposes (Stannard et al 1985;Nakamura 1987;Lazarova and Savoys 2004); 254-nm radiation is one of the most frequently used laboratory treatments to characterize the microbial response to DNA-damaging agents (reviewed in Coohill and Sagripanti 2008). Due to experimental limitations, these experiments are mainly focused on one growth phase of the selected microorganism, even though it is known that variations in sensitivity to UV (Morton and Haynes 1969) and other external stress factors exist (Keller and Maxcy 1984;Dowds et al 1987;DeVeaux et al 2007;Sukhi et al 2009) between the cells in diVerent growth phases.…”

Section: Introductionmentioning

confidence: 99%

Growth phase-dependent UV-C resistance of Bacillus subtilis: data from a short-term evolution experiment

et al. 2011

View full text Add to dashboard Cite

After 700 generations of a short-term evolution experiment with Bacillus subtilis 168, two strains were isolated, the UV-adapted strain MW01 and the UV-unexposed control strain DE69, and chosen for UV-C radiation resistance studies with respect to growth phase. The ancestral strain from the evolution experiment was used as reference for comparative purposes. Cells of the UV-adapted strain showed signiWcant diVerences in their physiology (growth behavior, doubling time, cell density, and sporulation capacity) and were more resistant to UV in all monitored stages. These Wndings implicate the evolution to an increased UV radioresistance was not limited to a speciWc growth phase and led to reduced growth dynamics, compared with those obtained from the ancestral and the control strain.

show abstract

“…UV radiation at a 254-nm wavelength has been used as an efficient and cost-effective means of disinfecting surfaces (1,4,15,16,17), building air (3,8,13), and drinking water supplies (5). The most reliable method for testing the efficacy of UV disinfection equipment is biodosimetry, the use of a test organism to measure the biologically effective UV dose (2).…”

mentioning

confidence: 99%

UV Resistance of Bacillus anthracis Spores Revisited: Validation of Bacillus subtilis Spores as UV Surrogates for Spores of B. anthracis Sterne

Nicholson

Galeano

2003

Appl Environ Microbiol

136

111

View full text Add to dashboard Cite

Recent bioterrorism concerns have prompted renewed efforts towards understanding the biology of bacterial spore resistance to radiation with a special emphasis on the spores of Bacillus anthracis. A review of the literature revealed that B. anthracis Sterne spores may be three to four times more resistant to 254-nmwavelength UV than are spores of commonly used indicator strains of Bacillus subtilis. To test this notion, B. anthracis Sterne spores were purified and their UV inactivation kinetics were determined in parallel with those of the spores of two indicator strains of B. subtilis, strains WN624 and ATCC 6633. When prepared and assayed under identical conditions, the spores of all three strains exhibited essentially identical UV inactivation kinetics. The data indicate that standard UV treatments that are effective against B. subtilis spores are likely also sufficient to inactivate B. anthracis spores and that the spores of standard B. subtilis strains could reliably be used as a biodosimetry model for the UV inactivation of B. anthracis spores.The October 2001 bioterrorist attack with Bacillus anthracis spores has sparked renewed interest in studying methods of bacterial spore inactivation and the mechanisms by which spores resist the lethal effects of various disinfection treatments. UV radiation at a 254-nm wavelength has been used as an efficient and cost-effective means of disinfecting surfaces (1,4,15,16,17), building air (3, 8, 13), and drinking water supplies (5). The most reliable method for testing the efficacy of UV disinfection equipment is biodosimetry, the use of a test organism to measure the biologically effective UV dose (2). Commonly used test organisms for UV biodosimetry studies are bacterial spores, usually spores of Bacillus subtilis, due to their high degree of UV resistance, reproducible inactivation response, and ease of use (reviewed in references 9 and 11). In particular, exhaustive testing of spores of the B. subtilis strain ATCC 6633 has resulted in this strain serving as the current European biodosimetry standard for 254-nm UV disinfection of drinking water (5).Semilogarithmic plots of spore inactivation versus UV fluence (dose) produce a characteristic curve, consisting of a shoulder at low UV doses, followed by a curve reflecting exponential inactivation at higher UV doses (Fig. 1). Two parameters often used to describe spore resistance to UV are (i) the UV dose lethal for 90% of the population (LD 90 ) and (ii) the decimal reduction value (D value), defined as the UV dose which reduces spore viability by a factor of 10, measured from the exponential portion of the inactivation curve (6, 11). For example, from the published data of Hoyer (5) (Fig. 1), it can be calculated that spores of B. subtilis ATCC 6633 exhibit an LD 90 and a D value of 260 and 120 J/m 2 , respectively (Table 1). In stark contrast to the extensively characterized UV inactivation response of B. subtilis spores, much less work has been performed to characterize the UV inactivation kinetics of B. anthracis ...

show abstract

Efficiency of Treatments Involving Ultraviolet Irradiation for Decontaminating Packaging Board of Different Surface Compositions

Cited by 15 publications

References 0 publications

Pulsed Ligh inactivation of Listeria innocua on food packaging materials of different surface roughness and reflectivity

Pulsed Ligh inactivation of Listeria innocua on food packaging materials of different surface roughness and reflectivity

Growth phase-dependent UV-C resistance of Bacillus subtilis: data from a short-term evolution experiment

UV Resistance of Bacillus anthracis Spores Revisited: Validation of Bacillus subtilis Spores as UV Surrogates for Spores of B. anthracis Sterne

Contact Info

Product

Resources

About