Pulsed Ligh inactivation of Listeria innocua on food packaging materials of different surface roughness and reflectivity

Ringus, Daina L.; Moraru, Carmen I.

doi:10.1016/j.jfoodeng.2012.08.022

Cited by 47 publications

(25 citation statements)

References 21 publications

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“…Pulsed-light technology has been found effective for eliminating bacteria and fungi on eggshells (28), on fruits (29), in fruit juices (30,31), on packaged chicken (32), and on food packaging materials (33). The FDA has recommended a total fluence up to 12 J Arrows: 1, intact particle; 2, apparently intact particles; 3, distorted particle; 4, debris; 5, empty particle.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and Mechanisms of Murine Norovirus Inhibition by Pulsed-Light Technology

Vimont

Fliss

Jean

2015

Appl Environ Microbiol

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Pulsed light is a nonthermal processing technology recognized by the FDA for killing microorganisms on food surfaces, with cumulative fluences up to 12 J cm ؊2 . In this study, we investigated its efficacy for inactivating murine norovirus 1 (MNV-1) as a human norovirus surrogate in phosphate-buffered saline, hard water, mineral water, turbid water, and sewage treatment effluent and on food contact surfaces, including high-density polyethylene, polyvinyl chloride, and stainless steel, free or in an alginate matrix. The pulsed-light device emitted a broadband spectrum (200 to 1,000 nm) at a fluence of 0.67 J cm ؊2 per pulse, with 2% UV at 8 cm beneath the lamp. Reductions in viral infectivity exceeded 3 log 10 in less than 3 s (5 pulses; 3.45 J cm ؊2 ) in clear suspensions and on clean surfaces, even in the presence of alginate, and in 6 s (11 pulses; 7.60 J cm ؊2 ) on fouled surfaces except for stainless steel (2.6 log 10 ). The presence of protein or bentonite interfered with viral inactivation. Analysis of the morphology, the viral proteins, and the RNA integrity of treated MNV-1 allowed us to elucidate the mechanisms involved in the antiviral activity of pulsed light. Pulsed light appeared to disrupt MNV-1 structure and degrade viral protein and RNA. The results suggest that pulsed-light technology could provide an effective alternative means of inactivating noroviruses in wastewaters, in clear beverages, in drinking water, or on food-handling surfaces in the presence or absence of biofilms.H uman noroviruses are responsible for one-fifth of all cases of acute gastroenteritis worldwide (1). They spread directly via person-to-person contact (fecal-oral and vomitus-oral) or indirectly through food, water, and the environment (2). Natural biofilms in sewage treatment effluent could be responsible for many persistent waterborne outbreaks, since such biofilms have been found to harbor noroviruses (3, 4). Infection spreads quickly and widely, affecting individuals of all ages, especially where many people gather or live in close proximity, such as in long-term care residences, retirement homes, cruise ships, and the like. Infectious doses as low as 2,800 particles, the high viral loads in feces and vomit (up to 10 9 genomic copies per gram), persistence in the environment, prolonged duration of viral shedding even after symptoms have resolved, and inadequate long-term immunity all contribute to the high incidence of norovirus illness (5). Although the illness is usually mild and self-limiting, the large number of cases per year represents a considerable loss in productivity and constitutes a substantial burden on society (2, 6).Among the physical disinfection processes, pulsed-light treatment appears well suited for the disinfection of food contact surfaces in industrial and health care settings and for decontaminating water and beverages. It has been proposed as a means of nonthermal pasteurization for food preservation and for decontaminating air and packaging materials (7). It has proven effective for inactivati...

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Section: Discussionmentioning

confidence: 99%

Efficacy and Mechanisms of Murine Norovirus Inhibition by Pulsed-Light Technology

Vimont

Fliss

Jean

2015

Appl Environ Microbiol

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“…Examples of reported facts are the polymer used, thickness of the material, light transmittance, supplier and product name (Fernández et al, 2009;Ganan, Hierro, Hospital, Barroso, & Fernández, 2013;Haughton et al, 2011;Hierro et al, 2011Hierro et al, , 2012Keklik, Demirci, & Puri, 2009Ramos-Villarroel, Aron-Maftei, Martín-Belloso, & Soliva-Fortuny, 2012a, 2012bRingus & Moraru, 2013). Hardly any of the authors, however, report more details like the additives and production process, possible laminates or co-extrudates and the properties of the individual layers or factors like roughness or reflectivity, which all could influence the efficacy of the decontamination.…”

Section: Efficacy Of Pulsed Light On Solid Matricesmentioning

confidence: 99%

“…Further, Ringus and Moraru (2013) reported that treatment of a low-density PE film coupon (40 μm) inoculated with Listeria innocua yields in the same decontamination (7 log CFU per coupon) whether treated with the inoculated surface to the PL source or upside down (Table 1).…”

Section: Efficacy Of Pulsed Light On Solid Matricesmentioning

confidence: 99%

“…Next to the surface decontamination of packaging materials and the link to material properties, Ringus and Moraru (2013) investigated the change in structural and physical properties of surface-treated lowand high-density polyethylene (LDPE and HDPE) as well as three laminates, namely MET, TR and EP. In their work, the water contact angle measurement was used as a measure of surface hydrophobicity to indicate changes in surface structure, barrier property and bacterial adherence (Bower, McGuire, & Daeschel, 1996;Dury-Brun, Chalier, Desorby, & Voilley, 2007;Li & Logan, 2005;Mafu, Roy, Goulet, & Savoie, 1991;Raab, Kotulák, Kolařík, & Pospíšil, 1982).…”

Section: Influence Of Pulsed Light On Packaging Materialsmentioning

confidence: 99%

“…Surface roughness (average, root mean square and ten point roughness) was tested to determine the possibility of cells shading or hiding from the treatment. Specular (light reflected at the same angle as the angle of incidence) and diffuse (light reflected at a different angle than the angle of incidence) reflection was used to confirm the negative influence of reflectivity on the decontamination (Ringus & Moraru, 2013;Woodling & Moraru, 2005). The findings showed that the most reflective materials, MET, EP and TR, also had the highest roughness and hence were lowest in enabling surface decontamination.…”

Section: Influence Of Pulsed Light On Packaging Materialsmentioning

confidence: 99%

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