Growth phase-dependent UV-C resistance of Bacillus subtilis: data from a short-term evolution experiment

Wassmann, Marko; Moeller, Ralf; Reitz, Günther; Rettberg, Petra

doi:10.1007/s00203-011-0722-9

Cited by 27 publications

(25 citation statements)

References 56 publications

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“…Strain MW01, an isolate from one of the evolved lines, was tested for UV resistance at five points in its life cycle: lag, exponential, and stationary phases; as mature endospores; and during germination. MW01 showed higher resistance than the ancestor in all growth phases and during germination, and in spores exposed to high UV fluxes (Wassmann et al, 2011). In the subsequent ADAPT experiment, MW01 spores were tested in Low Earth Orbit, exposed either to full spectrum solar radiation and high vacuum or to simulated martian conditions, with attenuated ultraviolet light and a low-pressure CO 2 atmosphere.…”

Section: Evolution With Selection For Uv Resistancementioning

confidence: 99%

Experimental evolution of Bacillus subtilis

Zeigler

Nicholson

2017

Environmental Microbiology

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Summary The endospore‐forming bacteria have persisted on earth perhaps 3Ga, leveraging the flexibility of their distinctive lifestyle to adapt to a remarkably wide range of environments. This process of adaptation can be investigated through the simple but powerful technique of laboratory evolution. Evolved strains can be analyzed by whole genome sequencing and an array of omics technologies. The intensively studied, genetically tractable endospore‐former, Bacillus subtilis, is an ideal subject for laboratory evolution experiments. Here, we describe the use of the B. subtilis model system to study the adaptation of these bacteria to reduced and stringent selection for endospore formation, as well as to novel environmental challenges of low atmospheric pressure, high ultraviolet radiation, and unfavourable growth temperatures. In combination with other approaches, including comparative genomics and environmental field work, laboratory evolution may help elucidate how these bacteria have so successfully adapted to life on earth, and perhaps beyond.

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Section: Evolution With Selection For Uv Resistancementioning

confidence: 99%

Experimental evolution of Bacillus subtilis

Zeigler

Nicholson

2017

Environmental Microbiology

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“…Martian atmosphere and pressure even led to no detectable effect. When exposed to space or Mars UV, the spores were inactivated by 4.5 and 1-2 orders of magnitude respectively, showing that Martian environmental conditions are less harmful for these spores than space conditions (Wassmann et al 2010(Wassmann et al , 2011(Wassmann et al , 2012.…”

Section: Exposementioning

confidence: 99%

Space as a Tool for Astrobiology: Review and Recommendations for Experimentations in Earth Orbit and Beyond

et al. 2017

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The space environment is regularly used for experiments addressing astrobiology research goals. The specific conditions prevailing in Earth orbit and beyond, notably the radiative environment (photons and energetic particles) and the possibility to conduct long-duration measurements, have been the main motivations for developing experimental concepts to expose chemical or biological samples to outer space, or to use the reentry of a spacecraft on Earth to simulate the fall of a meteorite. This paper represents an overview of past and current research in astrobiology conducted in Earth orbit and beyond, with a special focus on ESA missions such as Biopan, STONE (on Russian FOTON capsules) and EXPOSE facilities (outside the International Space Station). The future of exposure platforms is discussed, notably how they can be improved for better science return, and how to incorporate the use of small satellites such as those built in cubesat format.

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“…The life cycle of most micro-organisms comprises four phases; the lag phase, the exponential or log phase, the stationary phase and the death phase. Due to the expression of specific stress response genes, most bacteria possess their highest resistance against external stresses during the stationary phase [19]. That this also holds true in the case of plasma treatment was demonstrated by Yu et al [20], who reported differences in the inactivation rate constants for E. coli at mid-log, late log and stationary phases of growth.…”

Section: Introductionmentioning

confidence: 94%