Efficiency of MY09/11 consensus PCR in the detection of multiple HPV infections

Şahiner, Fatih; Kubar, Ayhan; Gümral, Ramazan; Ardıç, Medine; Yiğit, Nuri; Şener, Kenan; Dede, Murat; Yapar, Mehmet

doi:10.1016/j.diagmicrobio.2014.03.030

Cited by 21 publications

(19 citation statements)

References 23 publications

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“…MY09/ 11 has been shown to have lower sensitivity compared with other L1 consensus primer sets. 28,29 Owing to limitations of retrospective analyses, the significance of reported prevalence in this study is unclear. Risk factors, such as sexual history, were unable to be ascertained.…”

Section: Limitationsmentioning

confidence: 87%

Prevalence of High-Risk Human Papillomavirus in Tonsil Tissue in Healthy Adults and Colocalization in Biofilm of Tonsillar Crypts

Rieth

Gill

Lott-Limbach

et al. 2018

JAMA Otolaryngol Head Neck Surg

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Section: Limitationsmentioning

confidence: 87%

Prevalence of High-Risk Human Papillomavirus in Tonsil Tissue in Healthy Adults and Colocalization in Biofilm of Tonsillar Crypts

Rieth

Gill

Lott-Limbach

et al. 2018

JAMA Otolaryngol Head Neck Surg

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“…Each of these methods has some drawbacks, mainly in the area of reagents, cost effectiveness, time required, and false positive and negative results, especially in samples with lower copy numbers and mixed infections. Results of some surveys show that primers such as MY09/11 are ineffective due to increased mutations in HPV genome and multiple infections ( 65 - 66 ). Therefore, conventional PCR and other molecular methods should be improved in order to cover these new mutant types or to revise the screening methods by implementing other recommended molecular techniques for HPV detection and typing ( 61 , 65 , 66 ).…”

Section: Discussionmentioning

confidence: 99%

“…Results of some surveys show that primers such as MY09/11 are ineffective due to increased mutations in HPV genome and multiple infections ( 65 - 66 ). Therefore, conventional PCR and other molecular methods should be improved in order to cover these new mutant types or to revise the screening methods by implementing other recommended molecular techniques for HPV detection and typing ( 61 , 65 , 66 ). At present, valid genotyping procedures available commercially and in-house methods are approved in WHO proficiency testing, and could be considered as the best practical way for primary HPV testing in cervical cancer screening plans ( 10 , 66 ).…”

Section: Discussionmentioning

confidence: 99%

Cervical Cancer and Genital Infections: Assessment of Performance and Validation in Human Papillomavirus Genotyping Assays in Iran, its Neighbouring Countries and Persian Gulf Area

Sohrabi¹,

Hajia²

2017

Iran J Pathol

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Background: The accuracy of diagnostic assays in Human Papillomavirus (HPV) genital infection and cervical cancer has remained a clinical challenge in diagnosis. Evidence indicates that a large proportion of cervical cancer can be prevented through organized care for HPV and testing. Countries with low per capita income, such as Iran and its neighbours, have no national organized program for cervical cancer screening and vaccination. The aim of this study was to review recent published papers in this region for evaluating the efficacy of released data regarding HPV genotyping system in genital infections and cervical cancer Methods: Investigating various medical search engines retrieved 46 reports, mostly after 2010, consisting of either home brew protocols or commercial technologies in this field. Results: Summarized results demonstrated that except a few cases, all reports were limited studies performed in confined populations focusing on attending patients at clinics for regular checkups. In the present study, 52.8% of papers were from Iran and the rest belonged to other countries. The rate of HPV infection was reported in the range of 0.62% to 25% in the normal population, while it varied from 18.75% to 100% in females with cervical cancer. In HPV genotyping surveys, only 26.1 % (12/46) of reports had validated and World Health Organization (WHO) proficient procedures. Also, multiple infections were not mentioned in 56.52% (25/46) of researches. Conclusions: Employing reliable genotyping methods is the best way for regular screening of cervical cancer related to HPV and precancerous diseases in females of these areas. The focus of most surveys was to come up with the best national policies for establishing a preventive program in Iran and Persian Gulf area.

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“…Human β-globin housekeeping gene (248-bp) HMBB01 5ʹ-GCG ACC CAA TGC AAA TTG GT-3ʹ PC04 5ʹ-GAA GAG CCA AGG ACA GGT AC-3ʹ GH20 5ʹ-CAA CTT CAT CCA CGT TCA CC-3ʹ (11.6%); HPV-18, 7 (7.4%); HPV-31, 7 (7.4%); HPV-35, 7 (7.4%); HPV-68, 6 (6.3%); HPV-33, 4 (4.2%); HPV-82, 4 (4.2%); HPV-39, 3 (3.2%); and HPV-45, 2 (2.1%) (31). In this study, types 52 and 58 are more prevalent than type 18, which is different from the results of our study and the studies we compared our study with.…”

Section: Type Primersmentioning

confidence: 99%

Human papillomavirus prevalence and type in liquid-based cervical samples from Turkish women in a selected risk group

Akyar¹,

Aydın²,

Yakıcıer³

et al. 2013

Turk J Med Sci

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A series of solid-liquid phase change materials, thiourea derivatives, were prepared via condensation of thiourea with the respective carboxyl chlorides (lauroyl chloride, myristoyl chloride, and palmitoyl chloride) and were then characterized by using FT-IR, 1 H-NMR, differential scanning calorimetry (DSC), and thermogravimetric (TG) analysis. The thiourea derivatives (1,3-didodecanoyl thiourea, 1,3-ditetradecanoyl thiourea, and 1,3-dihexadecanoyl thiourea) were structurally symmetric and had long alkyl groups to crystallize. Thermal analysis by DSC and spectroscopic investigation by FT-IR spectroscopy were performed on the samples before and after thermal cycling tests to determine thermal reliability. The maximum latent heats of melting and freezing of the thiourea derivatives were found to be 114.6 and -110.0 J/g for 1,3didodecanoyl thiourea, 119.5 and -122.4 J/g for 1,3-ditetradecanoyl thiourea, and 148.8 and -142.7 J/g for 1,3-dihexadecanoyl thiourea after accelerated thermal cycling. A TG instrument was used to determine the starting point of degradation in the thiourea derivatives; it was found that the thiourea derivatives degraded at sufficiently higher temperatures than the expected utility temperatures.

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Efficiency of MY09/11 consensus PCR in the detection of multiple HPV infections

Cited by 21 publications

References 23 publications

Prevalence of High-Risk Human Papillomavirus in Tonsil Tissue in Healthy Adults and Colocalization in Biofilm of Tonsillar Crypts

Prevalence of High-Risk Human Papillomavirus in Tonsil Tissue in Healthy Adults and Colocalization in Biofilm of Tonsillar Crypts

Cervical Cancer and Genital Infections: Assessment of Performance and Validation in Human Papillomavirus Genotyping Assays in Iran, its Neighbouring Countries and Persian Gulf Area

Human papillomavirus prevalence and type in liquid-based cervical samples from Turkish women in a selected risk group

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