Efficiency of Antibiotic-loaded Polymethylmethacrylate Rods for Treatment of the Implant-related Infections in Rat Spine

Ofluoğlu, Ender; Bulent, Ekinci; Derya, Aydin M.; Sancar, Baris Y.; Gökçedağ, Akın; Tuğcu, Bekir; Emel, Erhan

doi:10.1097/bsd.0b013e3182425b93

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…The first was the model designed for rabbit spine by Poelstra et al (12). The other was the model designed for rat spine by Ofluoglu et al (11) and it was the first and only implant-related infection model designed for rat spine (10). This second model which was used in our study is based on the assumption that an implant infection can be created by inoculation of at least 10 6 colonies of MRSA.…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Günaldı¹,

Dağlıoğlu²,

Tuğcu³

et al. 2013

Turkish Neurosurgery

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AIm: Implant-related infections are still a significant problem in spinal surgical procedures. Many drugs and methods have been tried to prevent implant-related infections. Our objective in this study was to evaluate whether royal jelly, which was found to hinder the growth of MRSA, has any preventive role in the prognosis of an infection in rats in an implant-related infection model. mATeRIAL and meTHods: Rats were divided into 3 groups of eight rats. Group-1 consisted of rats that underwent only a spinal implant, group-2 included those rats that were inoculated bacteria together with a spinal implant and group-3 was administered royal jelly in addition to a spinal implant and infection. ResuLTs:The amount of bacteria that grew in vertebral columns and implants was more in Group-2 than in Group-3, which meant that the number of bacteria colonies that grew was more quantitatively. This difference was found to be statistically significant in vertebral columns, but not in implants.CoNCLusIoN: Royal jelly could not fully prevent the MRSA infection in this model, but decreased the severity of infection noticeably. More objective and promising results may be obtained if royal jelly can be used at regular intervals in a different model to be designed with respect to implant-related infections.KeywoRds: Implant, Infection, Royal jelly, Spine, Staphylococcus aureus, Rat ÖZ AmAÇ: Spinal cerrahi prosedürlerde implant ilişkili enfeksiyonlar, hala önemli yer tutmaktadır. İmplant ilişkili enfeksiyonları önlemeye yönelik birçok ilaç ve yöntem uygulanmıştır. Arı sütünün, in-vitro ve in-vivo şartlarda metisilin rezistan stafilokokus aureusun üremesini engellediği tespit edilmiştir. Bu çalışmada, implant ilişkili spinal enfeksiyon modelinde arı sütünün etkisini değerlendirdik. yÖNTem ve GeReÇLeR: Sıçanlar sekizerli 3 gruba ayrıldı. Grup-1; sadece spinal implant uygulanan grup, Grup-2; spinal implantla beraber bakteri ekilen grup, Grup-3; spinal implant ve enfeksiyona ilave arı sütü uygulanan grup. Grup-1'deki sıçanların hiç birinde doku, implant ve kan kültürlerinde bakteriyel üreme olmadı. Grup-2 ve 3'teki sıçanların tamamında ekimi yapılan standart MRSA suşu üredi. Gruplar birbirleri arasında enfeksiyon yoğunluğu açısından karşılaştırıldı.BuLGuLAR: Grup-2'deki vertebral kolon ve implantlar üzerinde üreyen bakteri sayısının grup-3'e kıyasla daha şiddetli olduğu, üreyen bakteri koloni sayısının kantitatif olarak daha fazla olduğu tespit edildi. Bu fark, vertebral kolonda istatistiksel olarak anlamlı bulunurken, implant üzerinde anlamlı bulunmadı. soNuÇ: Arı sütü bu modelde, MRSA enfeksiyonunu tam olarak önleyememiş ancak, enfeksiyonun şiddetini belirgin olarak azaltmıştır. Arı sütün implant ilişkili enfeksiyonlarda farklı bir model oluşturularak, düzenli aralıklarla kullanılabilmesi durumunda daha objektif ve umut verici sonuçlar ortaya konabilir. Ayrıca arı sütün, farklı modeller oluşturularak, farklı bakterilerde ve diğer Staph. aureus suşlarında da denenmesinin uygun olacağı kanaatindeyiz.

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Section: Discussionmentioning

confidence: 99%

“…The dead space in the surgical area, foreign bodies, necrotic tissue, and long-lasting surgical procedures are considered to be the factors increasing the risk of IRI (15). Clinicians have used many local and systemic agents to prevent IRIs (10). Royal jelly…”

Section: Sıçan Omurgasında İmplant İlişkili Spinal Enfeksiyonu öNlemementioning

confidence: 99%

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Günaldı¹,

Dağlıoğlu²,

Tuğcu³

et al. 2013

Turkish Neurosurgery

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“…In order to reduce the chances of post-operatory infection occurrence at surgical sites, various approaches have been attempted. Prophylactic parenteral antibiotics therapy [8][9][10] and antibiotic-releasing bone cement [11][12][13][14][15][16] have been successful; however, the use of antibiotics has given rise to bacterial strains resistant to such compounds. 17 Therefore, reliance on antibiotics cannot be seen as a long-term approach and the development of nonantibiotic antimicrobial techniques is extremely urgent.…”

Section: Introductionmentioning

confidence: 99%

A novel bone cement impregnated with silver–tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties

Prokopovich¹,

Leech²,

Carmalt³

et al. 2013

IJN

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Post-operatory infections in orthopedic surgeries pose a significant risk. The common approach of using antibiotics, both parenterally or embedded in bone cement (when this is employed during surgery) faces the challenge of the rising population of pathogens exhibiting resistance properties against one or more of these compounds; therefore, novel approaches need to be developed. Silver nanoparticles appear to be an exciting prospect because of their antimicrobial activity and safety at the levels used in medical applications. In this paper, a novel type of silver nanoparticles capped with tiopronin is presented. Two ratios of reagents during synthesis were tested and the effect on the nanoparticles investigated through TEM, TGA, and UV-Vis spectroscopy. Once encapsulated in bone cement, only the nanoparticles with the highest amount of inorganic fraction conferred antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) at concentrations as low as 0.1% w/w. No other characteristics of the bone cement, such as cytotoxicity or mechanical properties, were affected by the presence of the nanoparticles. Our work presents a new type of silver nanoparticles and demonstrates that they can be embedded in bone cement to prevent infections once the synthetic conditions are tailored for such applications.

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“…Many of these methods are based on the use of antibiotic-impregnated biomaterials, or topical and systemic agents. 31 , 32 Most studies have accepted that the use of ultraclean laminar flow ventilation, a sterile hood, and a body-exhaust system lead to a significant reduction in infections. Waterproof aprons have also been shown to be effective against the transfer of organisms between patients and surgeons.…”

Section: Discussionmentioning

confidence: 99%

Retrospective, Demographic, and Clinical Investigation of the Causes of Postoperative Infection in Patients With Lumbar Spinal Stenosis Who Underwent Posterior Stabilization

Yaldız

Ceylan

et al. 2015

Medicine

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Owing to the increasing population of elderly patients, a large number of patients with degenerative spondylosis are currently being surgically treated. Although basic measures for decreasing postoperative surgical infections (PSIs) are considered, it still remains among the leading causes of morbidity and mortality. The aim of this retrospective analysis is to present possible causes leading to PSI in patients who underwent surgery for lumbar degenerative spondylosis and highlight how it can be avoided to decrease morbidity and mortality.The study included 540 patients who underwent posterior stabilization due to degenerative lumbar stenosis between January 2013 and January 2014. The data before and after surgery was retrieved from the hospital charts. Patients with degenerative lumbar stenosis who were operated upon in this study had >2 levels of laminectomy and facetectomy. For this reason, posterior stabilization was performed for all the patients included in this study.Determining the causes of postoperative infection (PI) following spinal surgeries performed with instrumentation is a struggle. Seventeen different parameters that may be related to PI were evaluated in this study. The presence of systemic diseases, unknown glove perforations, and perioperative blood transfusions were among the parameters that increased the prevalence of PI. Alternatively, prolene sutures, double-layered gloves, and the use of rifampicin Sv (RIS) decreased the incidence of PI.Although the presence of systemic diseases, unnoticed glove perforations, and perioperative blood transfusions increased PIs, prolene suture material, double-layered gloves, and the use of RIS decreased PIs.

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Efficiency of Antibiotic-loaded Polymethylmethacrylate Rods for Treatment of the Implant-related Infections in Rat Spine

Abstract: This study shows the success of antibiotic-loaded polymethylmethacrylate rods for the treatment of the spinal implant infections. Further research involving clinical studies is required to increase the efficacy and the clinical applicability of the technique.

Cited by 10 publications

References 22 publications

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

A novel bone cement impregnated with silver–tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties

Retrospective, Demographic, and Clinical Investigation of the Causes of Postoperative Infection in Patients With Lumbar Spinal Stenosis Who Underwent Posterior Stabilization

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Efficiency of Antibiotic-loaded Polymethylmethacrylate Rods for Treatment of the Implant-related Infections in Rat Spine

Abstract: This study shows the success of antibiotic-loaded polymethylmethacrylate rods for the treatment of the spinal implant infections. Further research involving clinical studies is required to increase the efficacy and the clinical applicability of the technique.

Cited by 10 publications

References 22 publications

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

A novel bone cement impregnated with silver&ndash;tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties

Retrospective, Demographic, and Clinical Investigation of the Causes of Postoperative Infection in Patients With Lumbar Spinal Stenosis Who Underwent Posterior Stabilization

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A novel bone cement impregnated with silver–tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties