Efficacy of transforming growth factor- β on development of ameloblasts and odontoblasts in tooth germ of young albino rat

El-Zainy, Medhat A.; El-Haddad, Khaled Nour; Obeid, Raneem F.

doi:10.1016/j.fdj.2017.08.001

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Euthanasia At the fourth day after birth, pups of each group were euthanized, decapitated and the mandible of each rat was dissected out. Mandibles were prepared for histological and ultrastructural examination (19) (20).…”

Section: Methodsmentioning

confidence: 99%

Effect of Maternal Vitamin D3 Deficiency on Dentinogenesis in Rats (Experimental Study)

Saleh¹,

elnozahy

moustafa

et al. 2022

Alexandria Dental Journal

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INTRODUCTION:Vitamin D3 insufficiency has become a severe health problem that affects more than one billion people worldwide. Maternal vitamin D3 deficiency can induce dentin defects during tooth development. OBJECTIVES: To evaluate the biological effect of maternal vitamin D3 deficiency on dentinogenesis of the rat offspring. MATERIALS AND METHODS: Twelve female Albino rats were used. Rats were divided randomly into two equal groups. In the control group, rats were fed normal balanced diet and exposed to the normal light/dark cycle. In the study group, rats were housed in incandescent light devoid of ultraviolet B-light and fed a diet free of vitamin D3.Vitamin D3 level in the serum was measured every two weeks till reached vitamin D3 deficiency. Female rats were mated to be pregnant. At the fourth day after labor, two pups were taken from the litter of each mother. The dentinogenesis of the developing mandibular first molars was examined by the light and transmission electron microscope and evaluated by histomorphometric analysis. RESULTS: Vitamin D3 deficiency harmfully affected odontoblast differentiation, dentin formation and mineralization. CONCLUSION: Maternal vitamin D3 deficiency during tooth development has an adverse effect on odontoblast differentiation, dentin formation and mineralization.

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Section: Methodsmentioning

confidence: 99%

Effect of Maternal Vitamin D3 Deficiency on Dentinogenesis in Rats (Experimental Study)

Saleh¹,

elnozahy

moustafa

et al. 2022

Alexandria Dental Journal

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“…TGF-β plays an important role in growth arrest of normal and cancer cells (27). In vivo experiment, TGF-β1 ligand promotes the maturation stage of enamel development and induces odontoblast differentiation and dentin mineralization (38). To initiate the TGF-β pathway, two receptors, TGF-β type I (TβRI) and type II (TβRII) receptors embedded in the cell surface were involved in the binding of TGF-β ligand.In brief, ligand binding to receptors activates the TGF-pathway, which then recruits and collaborates with the Smad pathway to promote cell growth arrest and cell differentiation (14) by translocating these complexes into the nucleus.…”

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confidence: 99%

Histone deacetylase inhibitor induces the odontogenic differentiation in human dental pulp cells: comparison of trichostatin a (TSA) and suberoylanilide hydroxamic acid (SAHA)

Sulistyowati¹

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Human dental pulp cells (hDPCs) have shown their plasticity to differentiate into odontoblast-like cell lineages under the treatment of two-members of hydroxamates HDAC inhibitors (HDACis), Trichostatin A (TSA) and Suberoylanilide hydroxamic acid (SAHA). However, a comparison of the potency for stimulating odontoblast-like differentiation and mineralization process among these two HDACis has not been reported. Therefore, we aimed to confirm and compare the stimulatory effect of TSA and SAHA in inducing odontoblast-like differentiation and promoting mineralized-nodule formation. The primary cultured hDPCs was used for MTT assay, ALP activity assay, and alizarin red staining in the presence and absence of TSA and SAHA, with various concentration. Odontoblast-related gene expression was observed by RT-qPCR. Scratch wound healing assay was performed to observe the TSA and SAHA effects on cell migration. Either TSA or SAHA treatment have no effect on hDPCs viability as observed by MTT assay. The presence of TSA and SAHA induced odontoblast-like differentiation, confirmed by; the significant increase of ALP activity and mineral deposition during TSA 400 nM or SAHA 1µM treatment, the significant acceleration pattern of NFI-C, KLF4, DMP1, DSPP, COL1, ALP, BSP, OC, VEGF, and p21 gene expressions analyzed by RT-qPCR, at 24h, 72h, 7d and 5d. Furthermore, Scratch wound healing assay displayed the enhanced cell migration at 72h after TSA or SAHA treatment. Our findings showed that TSA and SAHA might have similar stimulatory effect in inducing odontogenic differentiation and mineralization process of hDPCs and proposed another potential application of TSA and SAHA to promote dentin regeneration.

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Building platelet phenotypes: Diaphanous-related formin 1 (DIAPH1)-related disorder

Rabbolini

Liang²,

Connor³

et al. 2021

Platelets

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Efficacy of transforming growth factor- β on development of ameloblasts and odontoblasts in tooth germ of young albino rat

Cited by 3 publications

References 20 publications

Effect of Maternal Vitamin D3 Deficiency on Dentinogenesis in Rats (Experimental Study)

Effect of Maternal Vitamin D3 Deficiency on Dentinogenesis in Rats (Experimental Study)

Histone deacetylase inhibitor induces the odontogenic differentiation in human dental pulp cells: comparison of trichostatin a (TSA) and suberoylanilide hydroxamic acid (SAHA)

Building platelet phenotypes: Diaphanous-related formin 1 (DIAPH1)-related disorder

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