Efficacy of the chemical trifluoromethanesulfonamide as a male gametocide in field-grown sorghum [Sorghum bicolor (L.) Moench]

Boerman, Nicholas A.; Hlavinka, Kyle B.; Zhu, Weixi; Dabney, Alan R.; Hodnett, George L.; Rooney, William L.

doi:10.1007/s10681-019-2417-2

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…In the current study, recovery of pollen fertility after TFMSA treatment was observed, and was directly related to the applied dosage. This observation supports the findings of Boerman et al ( 2019 ) and Hodnett and Rooney ( 2018 ) regarding reversion of TFMSA induced male sterility, whereby cowpea can recover its pollen functionality after 2 months, especially from relatively lower doses. Proline is required for male fertility in A. thaliana (Mattioli et al 2012 ).…”

Section: Discussionsupporting

confidence: 91%

“…The two-time treatment with 30 ml of 1000 mg/l (w/v) TFMSA solution (60 ml per plant in total) had no adverse effect on cowpea female functionality, confirming its male-specific gametocidal effect (Loussaert 2004 ). This is in agreement with the effects of TFMSA in sorghum and maize (Boerman et al 2019 ; Hodnett and Rooney 2018 ; Kyanam et al 2021 ; Loussaert 2004 ). The hybrid cowpea plants did not show any growth impairment, suggesting that TFMSA did not have any genetic effect on female cowpea gametes.…”

Section: Discussionsupporting

confidence: 87%

“…The induction of complete male sterility by treatment with TFMSA appears to be temporary, with reversion to pollen fertility as demonstrated by self-pollination. This reflects the duration of its activity, and is likely dependent on the dosage and timing of application (Boerman et al 2019 ; Hodnett and Rooney 2018 ). Also, cowpea has indeterminate growth habit with the vegetative and reproductive stages occurring at the same time.…”

Section: Discussionmentioning

confidence: 99%

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Chemical emasculation in cowpea (Vigna unguiculata (L.) Walp.) and dicotyledonous model species using trifluoromethanesulfonamide (TFMSA)

2023

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Hybridization plays an indispensable role in creating the diversity associated with plant evolution and genetic improvement of crops. Production of hybrids requires control of pollination and avoidance of self-pollination for species that are predominantly autogamous. Hand emasculation, male sterility genes or male gametocides have been used in several plant species to induce pollen sterility. However, in cowpea (Vigna unguiculata (L.) Walp), a self-pollinated cleistogamous dryland crop, only hand emasculation is used, but it is tedious and time-consuming. In this study, male sterility was effectively induced in cowpea and two dicotyledonous model species (Arabidopsis thaliana (L.) Heynh. and Nicotiana benthamiana Domin) using trifluoromethanesulfonamide (TFMSA). Pollen viability assays using Alexander staining showed that 30 ml of 1000 mg/l TFMSA with two-time treatments of one-week interval at the early stage of the reproductive phase under field or greenhouse conditions induced 99% pollen sterility in cowpea. TFMSA treatment induced non-functional pollen in diploid A. thaliana at two-time treatment of 10 ml of 125–250 mg/l per plant and N. benthamiana at two-time treatment of 10 ml of 250–1000 mg/l per plant. TFMSA-treated cowpea plants produced hybrid seeds when used as the female parent in crosses with non-treated plants used as male parents, suggesting that TFMSA had no effect on female functionality in cowpea. The ease of TFMSA treatment and its effectiveness to induce pollen sterility in a wide range of cowpea genotypes, and in the two model plant species tested in this study, may expand the scope of techniques for rapid pollination control in self-pollinated species, with potential applications in plant breeding and plant reproduction science.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Chemical emasculation in cowpea (Vigna unguiculata (L.) Walp.) and dicotyledonous model species using trifluoromethanesulfonamide (TFMSA)

2023

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“…All TFMSA applications used a standard backpack sprayer in a solution with TFSMA and a spreader as described by Boerman et al. (2019). The sprayer nozzle was adjusted to spray a fine mist and calibrated to apply 3.75 ml per second at a constant pressure of 15 PSI.…”

Section: Methodsmentioning

confidence: 99%

Analysis of effects of trifluoromethanesulfonamide in sorghum inbred lines and their derived hybrids

2021

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In sorghum [Sorghum bicolor (L.) Moench], development of male‐sterile versions (A‐lines) of the seed parents (B‐lines) is a tedious and time‐consuming process limiting opportunities for the improvement of new seed parents. Assessment of the value of a potential new seed parent requires testcross hybrid evaluations, but because of male fertility of these lines, conventional methods are inadequate to generate hybrid seed required for testing. Mechanisms to reduce the number of lines that require sterilization would be beneficial. The chemical gametocide trifluoromethanesulfonamide (TFMSA) was evaluated for its potential applicability to generate testcross hybrid seed. To study the effect on inbred line productivity, foliar applications of 25 mg of TFMSA were made to a select set of male‐fertile B‐lines and were compared to the corresponding untreated male‐sterile A‐line. Trifluoromethanesulfonamide induced near‐complete male‐sterility and allowed for testcross hybrid seed production across a range of genotypes. Comparison to untreated B‐lines indicated an average 3‐d delay in flowering and a 13‐cm reduction in plant height. Furthermore, average seed weight per panicle (productivity) was reduced by 8 g. Comparison of testcross hybrids produced by TFMSA induced male‐sterile lines and cytoplasmic male‐sterile lines did not reveal any significant and meaningful differences between the hybrids derived from both sterility systems. Moreover, inbred lines produced hybrids that ranked consistently in both systems. These results show TFMSA can affect productivity of treated inbred lines, but not the derived hybrid, which demonstrates that TFMSA can be used to produce hybrids for evaluation prior to investment in sterilizing of these lines.

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Methodologies for hybridization in predominantly self-pollinating crops: a review

Yahaya

Shimelis

Laing

et al. 2019

Journal of Crop Improvement

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Efficacy of the chemical trifluoromethanesulfonamide as a male gametocide in field-grown sorghum [Sorghum bicolor (L.) Moench]

Cited by 3 publications

References 19 publications

Chemical emasculation in cowpea (Vigna unguiculata (L.) Walp.) and dicotyledonous model species using trifluoromethanesulfonamide (TFMSA)

Chemical emasculation in cowpea (Vigna unguiculata (L.) Walp.) and dicotyledonous model species using trifluoromethanesulfonamide (TFMSA)

Analysis of effects of trifluoromethanesulfonamide in sorghum inbred lines and their derived hybrids

Methodologies for hybridization in predominantly self-pollinating crops: a review

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