Efficacy of taurolidine against periodontopathic species—an in vitro study

Abstract

The antimicrobial effect of taurolidine was tested against periodontopathic species in comparison to chlorhexidine digluconate in the presence or absence of serum. Minimal inhibitory concentrations (MIC), microbiocidal concentrations (MBC), as well as killing were determined against 32 different microbial strains including 3 Porphyromonas gingivalis, 3 Aggregatibacter actinomycetemcomitans, and 15 potentially superinfecting species with and without 25% v/v human serum. The MIC 50 of taurolidine against the tested microbial strains was 0.025% and the MIC 90 0.05%. The respective values for the MBCs were 0.05% and 0.1%. Addition of 25% serum (heat-inactivated) did not change the MIC and MBC values of taurolidine. In contrast, MICs and MBCs of chlorhexidine (CHX) increased by two steps after addition of serum. Taurolidine killed microorganisms in a concentration and timedependent manner, the killing rate of 1.6% taurolidine was 99.08%±2.27% in mean after 2 h. Again, killing activity of taurolidine was not affected if serum was added, whereas addition of inactivated serum clearly reduced the killing rate of all selected bacterial strains by CHX. Therefore, taurolidine possesses antimicrobial properties which are not reduced in the presence of serum as a main component in gingival crevicular fluid and wound fluid. Taurolidine may have potential as an antimicrobial agent in non-surgical and surgical periodontal treatment.

In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

“…The obtained values related to the available formulations revealed extremely low MICs of CHX and confirms findings from other studies [20,21]. However, it should be mentioned that in the present study CHX was tested without any additives.…”

In vitro activity of taurolidine gel on bacteria associated with periodontitis

“…Taurolidine in gel formulations showed favorable MIC values against the included single species with and without serum and were in the range or slightly higher tested before for the 2 % solution [8,9] meaning that the additives of the gel do not Fig. 2 Numbers of viable bacteria of selected oral species (determined by colony forming unit counts) after 2 h of exposure to taurolidine gel formulations and minocycline microspheres and subsequent cultivation in antimicrobial free media interfere with the antimicrobial activity of taurolidine.…”

“…In a first in vitro study, we compared a 2 % taurolidine solution with a 0.1 % chlorhexidine digluconate solution in different dilutions [8]. The minimal inhibitory concentrations (MICs) of taurolidine were all below 5 % of the normally used concentration of that substance with the exception of Candida albicans.…”

Taurolidine as an effective and biocompatible additive for plaque-removing techniques on implant surfaces

“…Because of these properties, T is used as an antiseptic solution in the treatment of peritonitis [31] and catheter locking solution [32,33]. In dentistry, the antimicrobial effect of T was investigated and proved on several bacterial strains [34] as well as on in vivo collected supragingival plaque biofilm samples [35]. Also, the higher biocompatibility of T in comparison to chlorhexidine to human gingival fibroblasts and osteoblast-like SAOS-2 cells for the time of a mouth rinse on titanium surfaces of dental implants was demonstrated [36].…”