In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

Jurczyk, K.; Nietzsche, Sándor; Ender, Claudia; Sculean, Anton; Eick, Sigrun

doi:10.1007/s00784-016-1711-9

Cited by 44 publications

(36 citation statements)

References 41 publications

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“…Interestingly, the same antiseptics in aqueous gel that form more quickly lost their capacity to interfere in biofilm formation and elimination. This finding contradicts expectations that a semisolid can remain longer on the wound surface (42,43). The choice of the optimal vehicle(s) used during gel formulation is a critical issue for wound care.…”

Section: Discussionmentioning

confidence: 72%

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

Ortega‐Peña

Hidalgo-González

Robson

et al. 2016

International Wound Journal

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Topical antiseptics are widely used for wound treatment, with the goal of disrupting biofilm capacity. We analysed the effectiveness of a variety of antiseptics to inhibit various stages of biofilm formation and to remove biofilms in vitro as well as the agents' cytotoxic effects on fibroblasts. We found that the chlorine-releasing agents exhibited immediate anti-biofilm effects in the short term, with lesser cytotoxicity than agents prepared from more stable compounds, such as biguanide or modified diallyl disulfide-oxide, which, conversely, have better long-term effectiveness. Among the examined organisms, Gram-positive bacteria and Candida albicans were the most sensitive to the antiseptics, whereas Pseudomonas aeruginosa and Acinetobacter baumannii were relatively resistant to them. Formulations whose mechanisms of action involve the release of chemically active chlorine were more effective when administered in solution than the gel form, likely because of the stability of the active ingredients during or after preparation of the formula. Interestingly, hypochlorous acid and some superoxidation solutions were effective in preventing biofilm formation within a short time period and showed virtually no toxicity. Our study indicates that most antiseptics remain effective long enough to prevent biofilm formation; thus, even brief application of an antiseptic agent during initial wound treatment can lead to better wound management outcomes.

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Section: Discussionmentioning

confidence: 72%

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

Ortega‐Peña

Hidalgo-González

Robson

et al. 2016

International Wound Journal

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“…gordonii suspension, 50 μ l of A. naeslundii suspension, and 100 μ l each of the suspensions of the other strains were mixed together before being diluted 1 to 10. The different μ l volumes were used to avoid streptococci inhibiting anaerobic bacterial growth, establishing a biofilm containing all investigated bacteria [24]. …”

Section: Methodsmentioning

confidence: 99%

“…For controls, ethanol (10–20%), chlorhexidine (0.05–0.2%; CHX), cetylpyridinium chloride (0.01–0.05%; CPC), pegylated hydrogenated castor oils (0.1–10%; Cremophor® RH 40; PEG), sodium dodecyl sulfate (0.25–1.0%; SDS), and SF (125–500 ppm) were used (all Sigma-Aldrich, St. Louis, MO). After incubation took place at 37°C in the indicated atmosphere for 18 to 42 hours, the inhibition zones were measured [24]. …”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity and Antimicrobial Activity of Oral Rinses In Vitro

Müller

Eick

Moritz

et al. 2017

BioMed Research International

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While oral rinses used for cosmetic purposes only do not necessarily have to be antiseptic, antimicrobial activity is required for medical indications, including oral and periodontal surgery. So the question arises—is the antimicrobial activity of oral rinses associated with any destructive changes in cell viability in vitro? To answer this question, we examined twelve oral rinses with respect to their antimicrobial and cytotoxic activity. Antimicrobial activity was screened against five bacterial strains using disc diffusion. Cytotoxicity was determined by mitochondrial reductase activity with primary gingival fibroblasts, L929 cells, and HSC-2 epithelial cells. Phase contrast microscopy and trypan blue staining were then performed to reveal cell morphology. Cells remained vital after exposure to oral rinses that were only used for cosmetic purposes. Moderate cytotoxic effects were observed for oral rinses containing 0.05% chlorhexidine, ethanol, or pegylated hydrogenated castor oil and sodium dodecyl sulfate. Other oral rinses containing 0.2% chlorhexidine and cocamidopropyl betaine exhibited strong cytotoxic and antimicrobial activity. Strong cytotoxic but moderate antimicrobial activity was observed in oral rinses containing cetylpyridinium chloride. The in vitro data show that oral rinses are heterogeneous with respect to their cytotoxic and antimicrobial effects. Based on their respective properties, oral rinses can be selected either to reduce the microbial load or for cosmetic purposes.

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“…Further studies will be necessary in order to assess the bactericidal activity of this device within a limited timeframe (comparable to the 10-60 seconds of a possible in vivo application). However, other antimicrobials commonly used in periodontitis have previously been evaluated for their in vitro activity by measurements of MIC and MBC [18,19]. Moreover, HybenX ® , at active concentrations, could be associated with specific dressings for ulcer treatment, therefore increasing the exposure time, as already done with other antimicrobial compounds [20].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Activity of the Decontaminant HybenX® Compared to Chlorhexidine and Sodium Hypochlorite against Common Bacterial and Yeast Pathogens

et al. 2019

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The recent increase in infections mediated by drug-resistant bacterial and fungal pathogens underlines the urgent need for novel antimicrobial compounds. In this study, the antimicrobial activity (inhibitory and cidal) of HybenX ® , a novel dessicating agent, in comparison with commonly used sodium hypochlorite and chlorhexidine, against a collection of bacterial and yeast strains representative of the most common human pathogenic species was evaluated. The minimal inhibitory, bactericidal, and fungicidal concentrations (MIC, MBC, and MFC, respectively) of the three different antimicrobial agents were evaluated by broth microdilution assays, followed by subculturing of suitable dilutions. HybenX ® was active against 26 reference strains representative of staphylococci, enterococci, Enterobacterales, Gram-negative nonfermenters, and yeasts, although at higher concentrations than sodium hypochlorite and chlorhexidine. HybenX ® MICs were 0.39% for bacteria (with MBCs ranging between 0.39% and 0.78%), and 0.1-0.78% for yeasts (with MFCs ranging between 0.78% and 1.6%). HybenX ® exhibited potent inhibitory and cidal activity at low concentrations against several bacterial and yeast pathogens. These findings suggest that HybenX ® could be of interest for the treatment of parodontal and endodontic infections and also for bacterial and fungal infections of other mucous membranes and skin as an alternative to sodium hypochlorite and chlorhexidine.

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In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

Cited by 44 publications

References 41 publications

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

Cytotoxicity and Antimicrobial Activity of Oral Rinses In Vitro

In Vitro Antimicrobial Activity of the Decontaminant HybenX® Compared to Chlorhexidine and Sodium Hypochlorite against Common Bacterial and Yeast Pathogens

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