Efficacy of synbiotic Jerusalem artichoke and Lactobacillus rhamnosus GG-supplemented diets on growth performance, serum biochemical parameters, intestinal morphology, immune parameters and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.)

Sewaka, Mariya; Trullàs, Clara; Chotiko, Arranee; Rodkhum, Channarong; Chansue, Nantarika; Boonanuntanasarn, Surintorn; Pirarat, Nopadon

doi:10.1016/j.fsi.2018.11.026

Cited by 75 publications

(43 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histological analysis of the intestinal mucosa can provide important information on fish nutritional status (Dimitroglou et al, ). In this study, fish fed the synbiotic diet showed increased villus height and robust goblet cells, consistent with the findings of Sewaka et al (), who observed significant increases in red tilapia intestinal villus length and width following supplementation with a synbiotic (Jerusalem artichoke and Lactobacillus rhamnosus ). There was a positive correlation between growth performance parameters (weight gain and FCR) and intestinal villus enhancement.…”

Section: Discussionsupporting

confidence: 92%

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

et al. 2019

View full text Add to dashboard Cite

This study investigated the effects of a synbiotic Lacto Forte on growth performance, haemato-immunological responses, plasma bactericidal capacity, histological profiles and resistance of Oreochromis niloticus to Pseudomonas fluorescens. The experimental fish were divided into three groups; two of them (T1 and T2) were given Lacto Forte incorporated diets at increasing level (0.7 and 1.5 g/kg, respectively) and an additive-free basal diet served as the control (T0) for 30 and 45 days. At the end of the feeding trial, a challenge was performed using a virulent strain of P. fluorescens and mortalities were recorded over an additional 14-days period. The results showed that Lacto Forte has a pronounced effect on haematological and growth performance parameters at 1.5 g/kg rather than 0.5 g/kg (p < 0.05). Plasma lysozyme, proteases, antiproteases and bactericidal capacity were significantly enhanced (p < 0.05) in Lacto Forte-treated groups, particularly after 45 days of supplementation. Long-term supplementation with Lacto Forte (1.5 g/kg) induced degenerative changes in the liver, spleen and intestine. Additionally, a significant increase (p < 0.05) in survival rates were found in Lacto Forte-treated groups compared to the control one 14 days post-challenge. As a result, Lacto Forte fortifies tilapia immune response and can be used as a surrogate for antibiotics to control P. fluorescens. K E Y W O R D S bactericidal, growth performance, Innate immunity, Oreochromis niloticus, Pseudomonas fluorescens, synbiotic Lacto Forte How to cite this article: Ismail M, Wahdan A, Yusuf MS, Metwally E, Mabrok M. Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease in Oreochromis niloticus. Aquac Res. 2019;50:2545-2562.

show abstract

Section: Discussionsupporting

confidence: 92%

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

et al. 2019

View full text Add to dashboard Cite

show abstract

“…This phenomenon was not seen in crayfish not fed synbiotics. This study is in line with Sewaka et al (2019) who studied the JA + LGG synbiotic supplementation in tilapia. Due to the development of the intestinal villi is better than the treatment without synbiotics, synbiotics can expand the nutrient absorption areas and more effective.…”

Section: Discussionsupporting

confidence: 88%

“…This present finding also suggests that probiotic and prebiotic are synergistic combination obtained from one of supplement nutrient. Thus, this synergistic combination can be alternative preventive measures and control of bacterial disease in fish farming (Sewaka et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Synbiotics have demonstrated more effective and long-lasting effects on growth and immunological responses (Van Doan et al, 2016;Wang et al, 2017;Safari & Paolucci, 2017;Hindu et al, 2018). Similarly, Sewaka et al (2019) found that the synbiotic application on the juvenile of red tilapia (Oreochromis spp) caused the intestines villus becoming high and wide. This leads to the expansion of food absorption area and feeds efficiency.…”

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Immune response and growth performance of crayfish Cherax quadricarinatus fed with supplementary diet of synbiotic

Amrullah¹,

Wahidah²

2019

Jurnal Akuakultur Indonesia

View full text Add to dashboard Cite

This study aimed to investigate the effectiveness of synbiotic (prebiotic alginate and probiotic bacteria Micrococcus spp) on the immune responses, growth performance, and bacterial resistance of freshwater crayfish. The experimental diets were formulated in three levels of synbiotic: the probiotic bacteria Micrococcus spp+100 mg/L alginate (Syn100), Micrococcus spp+200 mg/L alginate (Syn200), Micrococcus spp+300 mg/L alginate (Syn300), each level was mixed with tested feed. The basal diet (without synbiotics) served as the control diet. During 40 days of rearing, immune responses observations were conducted every 10 days, while the crayfish weight were recorded on day 40. Furthermore, the challenge test was performed against the pathogenic bacteria of A. hydrophila and reared for a week. The result showed that synbiotics treatment of Syn300 and Syn200 could improve the immune response and increased the growth rate (P<0.05). Both were also heavier than the Syn100 treatment. The highest resistance to A. hydrophila pathogenic bacteria by demonstrating a higher survival rate was Syn300 and followed by Syn200 (70.00 ± 0% and 73.33 ± 5.77%, respectively), compared to Syn100 (56.67 ± 3.33%) and the control (33.33 ± 3.33%). The synbiotic Micrococcus spp. combined with alginate potentially induced immune responses, increased growth performance, and improved bacterial pathogens resistance, making it an ideal synbiotic to be developed. Keywords: synbiotic, prebiotic, probiotic, Sargassum sp., alginate, brown algae ABSTRAK Penelitian ini bertujuan untuk menguji efektivitas penggunaan sinbiotik (prebiotik alginat dan probiotik Micrococcus spp) terhadap respons imun, performa pertumbuhan dan resistensi lobster air tawar. Tiga perlakuan sinbiotik yaitu Micrococcus spp+100 mg/L alginat (Syn100), Micrococcus spp+200 mg/L alginat (Syn200), Micrococcus spp+300 mg/L alginat (Syn300), masing-masing dicampurkan ke dalam pakan dan diberikan ke lobster. Pakan tanpa campuran prebiotik digunakan sebagai pakan kontrol. Selama 40 hari pemeliharaan, pengamatan respon imun dilakukan setiap 10 hari dan pada hari ke-40 pemeliharaan dilakukan pengukuran bobot lobster. Selanjutnya, lobster di uji tantang dengan bakteri A. hydrophila dan dipelihara kembali selama satu minggu. Hasil penelitian menunjukkan bahwa pemberian pakan yang mengandung sinbiotik Syn300 dan Syn200 dapat meningkatkan respon imun dan pertumbuhan (P<0.05) lebih tinggi dibandingkan dengan perlakuan Syn100, sementara bobot tubuh terendah didapat pada perlakuan kontrol. Resistensi lobster tertinggi terhadap bakteri A. hydrophila yang ditunjukkan dengan sintasan tertinggi didapatkan pada perlakuan Syn300 (70.00±0.00%) dan Syn200 (73.33±5.77%) dibandingkan dengan perlakuan Syn100 (56.67±3.33%) dan kontrol (33.33±3.33%). Penelitian ini membuktikan bahwa sinbiotik Micrococcus spp. yang dikombinasikan dengan alginat dapat menginduksi respons imun, meningkatkan performa pertumbuhan ,dan resistensi terhadap patogen sehingga merupakan sinbiotik yang ideal untuk dikembangkan. Kata kunci : sinbiotik, prebiotik, probiotik, Sargassum sp., alginat, alga coklat

show abstract

“…Of particular note, the similar results obtained in this study indicated that few inflammatory cells infiltrated the mucosa in the B. velezensis + A. veronii groups, and numerous goblet cells accumulated in the intestinal villi (Figure 9). Research verified that supplementation Lactobacillus rhamnosus could effectively promote intestinal health by increasing the population of intraepithelial lymphocytes and the villous height in tilapia model (Pirarat et al, 2011; Sewaka et al, 2019).…”

Section: Discussionmentioning

confidence: 89%

Effect of Bacillus velezensis on Aeromonas veronii-Induced Intestinal Mucosal Barrier Function Damage and Inflammation in Crucian Carp (Carassius auratus)

et al. 2019

View full text Add to dashboard Cite

Aeromonas veronii is an emerging aquatic pathogen causing hemorrhagic septicemia in humans and animals. Probiotic is an effective strategy for controlling enteric infections through reducing intestinal colonization by pathogens. Here we report that the consumption of Bacillus velezensis regulated the intestinal innate immune response and decreased the degree of intestinal inflammation damage caused by the A. veronii in Crucian carp. In this study, we isolated four strains of B. velezensis, named C-11, S-22, L-17 and S-14 from apparently healthy Crucian carp, which exerted a broad-spectrum antimicrobial activity inhibiting both Gram-positive and Gram-negative bacteria especially the fish pathogens. B. velezensis isolates showed typical Bacillus characteristics by endospore staining, physiological and biochemical test, enzyme activity analysis (amylase, protease, and lipase), and molecular identification. Here, Bacillus-containing dietary was orally administrated to Crucian carp for 8 weeks before A. veronii challenge. Immunological parameters and the expression of immune-related genes were measured at 2, 4, 6, 8, and 10 weeks post-administration. The results showed that B. velezensis was found to promote the increase in the phagocytic activities of peripheral blood leukocytes (PBLs) and head kidney leukocytes (HKLs), as well as the increase in interleukin 1β (IL-1β), IL-10 and tumor necrosis factor α (TNF-α) concentration of serum. Lysozyme levels (113.76 U/mL), ACP activity (25.32 U/mL), AKP activity (130.08 U/mL), and SOD activity (240.63 U/mL) were maximum (P < 0.05) in the B. velezensis C-11 treated group at 8 week. Our results showed that Crucian carp fed with the diet containing B. velezensis C-11 and S-22 developed a strong immune response with significantly higher (P < 0.05) levels of IgM in samples of serum, mucus of skin and intestine compared to B. velezensis L-17 and S-14 groups. Moreover, B. velezensis spores appeared to show no toxicity and damage in fish, which could inhabit the gut of Crucian carp. B. velezensis restrained up-regulation of pro-inflammation cytokines (IL-1β, IFN-γ, and TNF-α) mRNA levels in the intestine and head kidney at final stage of administration, and the expression of IL-10 was increased throughout the 10-week trial. A. veronii infection increased the population of inflammatory cells in the intestinal villi in the controls. In contrast, numerous goblet cells and few inflammatory cells infiltrated the mucosa in the B. velezensis groups after challenge with A. veronii. Compared with A. veronii group, B. velezensis could safeguard the integrity of intestinal villi. The highest post-challenge survival rate (75.0%) was recorded in B. velezensis C-11 group. The present data suggest that probiotic B. velezensis act as a potential gut-targeted therapy regimens to protecting fish from pathogenic bacteria infection.IMPORTANCEIn this work, four Bacillus velezensis strains isolated from apparently healthy Crucian carp, which exhibited a broad-spectrum antibacterial activity especially the...

show abstract

Efficacy of synbiotic Jerusalem artichoke and Lactobacillus rhamnosus GG-supplemented diets on growth performance, serum biochemical parameters, intestinal morphology, immune parameters and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.)

Cited by 75 publications

References 72 publications

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

Immune response and growth performance of crayfish Cherax quadricarinatus fed with supplementary diet of synbiotic

Effect of Bacillus velezensis on Aeromonas veronii-Induced Intestinal Mucosal Barrier Function Damage and Inflammation in Crucian Carp (Carassius auratus)

Contact Info

Product

Resources

About