Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease inOreochromis niloticus

Abstract

This study investigated the effects of a synbiotic Lacto Forte on growth performance, haemato-immunological responses, plasma bactericidal capacity, histological profiles and resistance of Oreochromis niloticus to Pseudomonas fluorescens. The experimental fish were divided into three groups; two of them (T1 and T2) were given Lacto Forte incorporated diets at increasing level (0.7 and 1.5 g/kg, respectively) and an additive-free basal diet served as the control (T0) for 30 and 45 days. At the end of the feeding trial, a challenge was performed using a virulent strain of P. fluorescens and mortalities were recorded over an additional 14-days period. The results showed that Lacto Forte has a pronounced effect on haematological and growth performance parameters at 1.5 g/kg rather than 0.5 g/kg (p < 0.05). Plasma lysozyme, proteases, antiproteases and bactericidal capacity were significantly enhanced (p < 0.05) in Lacto Forte-treated groups, particularly after 45 days of supplementation. Long-term supplementation with Lacto Forte (1.5 g/kg) induced degenerative changes in the liver, spleen and intestine. Additionally, a significant increase (p < 0.05) in survival rates were found in Lacto Forte-treated groups compared to the control one 14 days post-challenge. As a result, Lacto Forte fortifies tilapia immune response and can be used as a surrogate for antibiotics to control P. fluorescens. K E Y W O R D S bactericidal, growth performance, Innate immunity, Oreochromis niloticus, Pseudomonas fluorescens, synbiotic Lacto Forte How to cite this article: Ismail M, Wahdan A, Yusuf MS, Metwally E, Mabrok M. Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease in Oreochromis niloticus. Aquac Res. 2019;50:2545-2562.

Synbiotic Lactic Dry® enhanced the growth performance, growth-related genes, intestinal health, and immunity of Nile tilapia reared in inland brackish groundwater

“…In the current study, a synbiotic mixture was added to the basal diet of tilapia grown in inland brackish A powerful blend of probiotics (Saccharomyces cerevisiae, Lactobacillus acidophilus, Streptococcus faecium, and Bacillus subtilis) and prebiotics (mannan oligosaccharides (MOS) and β-glucan (BG)) at 0.5, 1, and 2 g/kg is feasibly enhanced the growth performance of Nile tilapia. Similar outcomes were illustrated in Nile tilapia fed dietary S. cerevisiae (Abass et al, 2018;Abdel-Tawwab et al, 2008;Khunrang et al, 2021), L. acidophilus (Hassaan et al, 2021a;Villamil et al, 2014), S. faecium (Lara-Flores et al, 2010;Lara-Flores et al, 2003), B. subtilis (Addo et al, 2017;Liu et al, 2017;Mohammadi et al, 2020), MOS (Cavalcante et al, 2020;El-Nobi et al, 2021) and BG (Ismail et al, 2019). The enhancement in the growth performance is also in line with increased feed intake and feed utilization (low FCR) under the present study conditions.…”

Potential of Pseudomonas putida probiotic feed mixture for improving growth, immune response, and disease resistance in Nile Tilapia compared to Saccharomyces cerevisiae probiotic

“…As a result, alternative approaches have been proposed, including bacteriophage, phytotherapy, probiotics, immunostimulants, and synbiotics (Aly 2009; Newaj‐Fyzul and Austin 2015; Mabrok and Wahdan 2018; Ismail et al. 2019; Yilmaz, 2019a, 2019b; Awad et al. 2022), which provide more environmentally friendly and sustainable means of controlling aquatic diseases.…”

“…Antibiotics have been widely used to combat bacterial diseases in aquaculture; however, their uncontrolled use and misuse have led to environmental problems and the emergence of antibiotic-resistant bacteria, leading to their ban in several countries (Ibrahem 2015). As a result, alternative approaches have been proposed, including bacteriophage, phytotherapy, probiotics, immunostimulants, and synbiotics (Aly 2009;Newaj-Fyzul and Austin 2015;Mabrok and Wahdan 2018;Ismail et al 2019;Yilmaz, 2019aYilmaz, , 2019bAwad et al 2022), which provide more environmentally friendly and sustainable means of controlling aquatic diseases.…”