Efficacy of oral prednisolone and dexamethasone in horses with recurrent airway obstruction in the presence of continuous antigen exposure

Leclère, Mathilde; Lefebvre-Lavoie, Josiane; Beauchamp, Guy; Lavoie, Jean‐Pierre

doi:10.1111/j.2042-3306.2009.00022.x

Cited by 36 publications

(31 citation statements)

References 25 publications

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“…Also, the experimental settings we used differed from those of Grossmann and colleagues [32], possibly also contributing to these differences. Our group recently reported a significant improvement in pulmonary function mediated by prednisolone and dexamethasone in heaves-affected horses, a disease that share many features of human asthma [33]. To achieve equivalent potencies, doses for both drugs administrated were calculated based on their oral bioavaibility and reported ratios [32].…”

Section: Discussionmentioning

confidence: 99%

Neutrophils Are Not Less Sensitive Than Other Blood Leukocytes to the Genomic Effects of Glucocorticoids

et al. 2012

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BackgroundNeutrophils are generally considered less responsive to glucocorticoids compared to other inflammatory cells. The reported increase in human neutrophil survival mediated by these drugs partly supports this assertion. However, it was recently shown that dexamethasone exerts potent anti-inflammatory effects in equine peripheral blood neutrophils. Few comparative studies of glucocorticoid effects in neutrophils and other leukocytes have been reported and a relative insensitivity of neutrophils to these drugs could not be ruled out.ObjectiveWe assessed glucocorticoid-responsiveness in equine and human peripheral blood neutrophils and neutrophil-depleted leukocytes.MethodsBlood neutrophils and neutrophil-depleted leukocytes were isolated from 6 healthy horses and 4 human healthy subjects. Cells were incubated for 5 h with or without LPS (100 ng/mL) alone or combined with hydrocortisone, prednisolone or dexamethasone (10−8 M and 10−6 M). IL-1β, TNF-α, IL-8, glutamine synthetase and GR-α mRNA expression was quantified by qPCR. Equine neutrophils were also incubated for 20 h with or without the three glucocorticoids and cell survival was assessed by flow cytometry and light microscopy on cytospin preparations.ResultsWe found that glucocorticoids down-regulated LPS-induced pro-inflammatory mRNA expression in both cell populations and species. These drugs also significantly increased glutamine synthetase gene expression in both equine cell populations. The magnitude of glucocorticoid response between cell populations was generally similar in both species. We also showed that dexamethasone had a comparable inhibitory effect on pro-inflammatory gene expression in both human and equine neutrophils. As reported in other species, glucocorticoids significantly increase the survival in equine neutrophils.ConclusionsGlucocorticoids exert genomic effects of similar magnitude on neutrophils and on other blood leukocytes. We speculate that the poor response to glucocorticoids observed in some chronic neutrophilic diseases such as severe asthma or COPD is not explained by a relative lack of inhibition of these drugs on pro-inflammatory cytokines expression in neutrophils.

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Section: Discussionmentioning

confidence: 99%

Neutrophils Are Not Less Sensitive Than Other Blood Leukocytes to the Genomic Effects of Glucocorticoids

et al. 2012

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“…Clinical signs and airway obstruction can be reversed by administration of corticosteroids, bronchodilators, or changing the environment 4, 5, 6. Recurrent airway obstruction principally affects horses over 7 years of age 7, 8.…”

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confidence: 99%

Inflammatory Airway Disease of Horses—Revised Consensus Statement

Couëtil

Cardwell

Gerber

et al. 2016

Veterinary Internal Medicne

338

662

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“…), RAO (Leclere et al . ) and infiltrative bowel disease (Barr ). Orally administered prednisolone has >50% bioavailability in both liquid and tablet forms and its anti‐inflammatory action has been demonstrated to extend for 12–24 h following a single 2.2 mg/kg bwt oral dose (Peroni et al .…”

Section: Commonly Used Glucorticoidsmentioning

confidence: 99%

Review of glucocorticoid therapy in horses. Part 1: Pharmacology

Cuming

Groover

Wooldridge

et al. 2016

Equine Veterinary Education

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Summary This article is the first in a three part review series examining glucocorticoid use in treatment of medical conditions and musculoskeletal disorders of the horse. This article provides a review of the structure and function of corticosteroids together with a summary of the available literature pertaining to the pharmacodynamics, pharmacokinetics and indications for use of, and adverse effects associated with, glucocorticoids currently available for use by equine veterinarians.

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Efficacy of oral prednisolone and dexamethasone in horses with recurrent airway obstruction in the presence of continuous antigen exposure

Abstract: Prednisolone was shown, for the first time, to our knowledge, to improve the pulmonary function of horses with RAO in the presence of continuous antigen exposure. This study also demonstrates the efficacy of low-dose oral dexamethasone in reversing airway obstruction in these conditions.

Cited by 36 publications

References 25 publications

Neutrophils Are Not Less Sensitive Than Other Blood Leukocytes to the Genomic Effects of Glucocorticoids

Neutrophils Are Not Less Sensitive Than Other Blood Leukocytes to the Genomic Effects of Glucocorticoids

Inflammatory Airway Disease of Horses—Revised Consensus Statement

Review of glucocorticoid therapy in horses. Part 1: Pharmacology

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