Efficacy of Low-Molecular-Weight Fucoidan as a Supplemental Therapy in Metastatic Colorectal Cancer Patients: A Double-Blind Randomized Controlled Trial

Tsai, Hsiang-Lin; Tai, Chi-Jung; Huang, Ching‐Wen; Chang, Fang-Rong; Wang, Jaw‐Yuan

doi:10.3390/md15040122

Cited by 102 publications

(72 citation statements)

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“…The literature suggests that fucoidan from brown algae could be a potential anticancer agent with promising bioactive effects and biocompatible properties [15]. A recent clinical study suggests that the administration of low molecular weight fucoidans to patients undergoing chemotherapy for metastatic colorectal cancer significantly improved the disease control rate compared to the control group [16]. The bioactive properties of fucoidan largely depend upon its structural characteristics.…”

Section: Discussionmentioning

confidence: 99%

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

et al. 2020

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Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 µg mL−1 and 93.62 ± 3.53 µg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals.

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Section: Discussionmentioning

confidence: 99%

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

et al. 2020

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“…Fucoidan extracts at a low molecular weight exhibit greater bioactivity, while high molecular weight extracts have poor solubility and poor penetration . Low‐molecular‐weight fucoidan (LMWF) has been shown to significantly improve disease control rate of metastatic colorectal cancer patients in a clinical trial, combined with chemotherapeutic agents . Some innovative technologies for fucoidan extraction have been developed to promote efficiency to obtain a better yield, with less time, smaller cost, and more environmentally friendly by lowering energy consumption .…”

Section: Discussionmentioning

confidence: 99%

“…38 Low-molecular-weight fucoidan (LMWF) has been shown to significantly improve disease control rate of metastatic colorectal cancer patients in a clinical trial, combined with chemotherapeutic agents. 39 Some innovative technologies for fucoidan extraction have been developed to promote efficiency to obtain a better yield, with less time, smaller cost, and more environmentally friendly by lowering energy consumption. 44 These include ultrasound-, microwave-, and enzyme-assisted technologies, which have been used in the extraction of fucoidan and other bioactive compounds from ocean algae and seaweed.…”

Section: Discussionmentioning

confidence: 99%

Fucoidan affects oral squamous cell carcinoma cell functions in vitro by regulating FLNA‐derived circular RNA

Zhang

Gao

Ren

et al. 2019

Annals of the New York Academy of Sciences

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Oral squamous cell carcinoma (OSCC) is one of the most common cancer types, with a high annual incidence. Although chemotherapy contributes to suppressing OSCC tumorigenesis, the available treatments result in poor prognosis because of local recurrence and regional lymph node metastasis. Thus, it is necessary to discover novel and safe drugs with greater effectiveness and fewer side effects. Fucoidan is a component of the cell wall of brown seaweed that has been shown to produce a wide range of biological activities. The present study aimed to investigate the effectiveness of fucoidan in treating OSCC. In in vitro studies, we found that fucoidan inhibited OSCC growth and suppressed migration and invasion of OSCC cells. In addition, the potential interaction between fucoidan and filamin A (FLNA)–derived circular RNA (circFLNA) was predicted using bioinformatics databases and then confirmed in OSCC samples and cell lines. Indeed, fucoidan increased the expression of circFLNA in OSCC cell lines. Furthermore, both fucoidan and circFLNA could mediate the expression of key proteins related to cell growth, apoptosis, migration, and invasion. In conclusion, our research demonstrated that fucoidan might be considered as a potential natural drug in the treatment of OSCC patients by targeting circFLNA.

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“…According to their molecular weight, fucoidans are classified into three classes: LMWF (<10 kDa), medium molecular weight fucoidan (MMWF) (10-10000 kDa), and HMWF (>10000 kDa) [31]. LMWF demonstrated better bioavailability and bioactivities than HMWF [178,179]. As a consequence, several articles reported physical, chemical and enzymatic modification of the native HMWF to get LMWF of higher biological activity [62].…”

Section: Enzymatic Modification Of Native Fucoidansmentioning

confidence: 99%

Fucoidans: Downstream Processes and Recent Applications

Zayed

Ulber

2020

Marine Drugs

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Fucoidans are multifunctional marine macromolecules that are subjected to numerous and various downstream processes during their production. These processes were considered the most important abiotic factors affecting fucoidan chemical skeletons, quality, physicochemical properties, biological properties and industrial applications. Since a universal protocol for fucoidans production has not been established yet, all the currently used processes were presented and justified. The current article complements our previous articles in the fucoidans field, provides an updated overview regarding the different downstream processes, including pre-treatment, extraction, purification and enzymatic modification processes, and shows the recent non-traditional applications of fucoidans in relation to their characters. of 22reproductive, immune and cell-to-cell communicative roles [23]. As recommended by the International Union of Pure and Applied Chemistry (IUPAC), fucoidans is a general term used to describe sulfated L-fucose-based polymers including sulfated fucans cited by the Swedish scholar Kylin, as well as other fucose-rich sulfated heteropolysaccharides [23,28]. Their chemical structures, in terms of monomeric composition and branching, are quite simple in marine invertebrates compared to their analogues in brown algae [13,29].Hundreds of articles have thoroughly discussed and reviewed the biological, pharmacological and pharmaceutical applications of fucoidans [30][31][32][33], including nanomedicine, [34] which has made it a hot topic in the last few decades [35][36][37]. All these studies tried to investigate fucoidans molecular mechanisms in relation to their chemical structure and physicochemical properties. Therefore, different hypotheses were suggested for each activity, such as anti-tumor [31,[38][39][40], anti-coagulant [41,42], anti-viral [43,44] and anti-inflammatory activity [45,46]. These investigations revealed that various factors are relevant, such as molecular weight, sulfation pattern, sulfate content and monomeric composition [47][48][49]. For example, different fractions were produced with different physicochemical properties in our previous experiments; sulfation pattern and sulfate content were highly related to anti-viral and cytotoxic activities against HSV-1 and Caco-2 cell lines, respectively, while molecular weight and sugar composition were potential factors in anti-coagulation activity [41,50]. In addition, degree of purity was reported as an influential factor [32], where co-extracted contaminants (e.g., phlorotannins or polyphenols) could lead to significant interference in anti-oxidant activity and, consequently, cosmetic applications [51,52].Therefore, several key production challenges regarding fucoidans were discussed in our last review article in order to obtain a product that follows the universal good manufactured practice (GMP) guidelines. The article discussed sources of heterogeneity in extracted fucoidans, including the different biotic (e.g., biogenic, geographical and seas...

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Efficacy of Low-Molecular-Weight Fucoidan as a Supplemental Therapy in Metastatic Colorectal Cancer Patients: A Double-Blind Randomized Controlled Trial

Cited by 102 publications

References 37 publications

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

Fucoidan affects oral squamous cell carcinoma cell functions in vitro by regulating FLNA‐derived circular RNA

Fucoidans: Downstream Processes and Recent Applications

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