Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

Abstract

Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 µg mL−1 and 93.62 ± 3.53 µg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals.

Purification and Structural Characterization of Sulfated Polysaccharides Derived from Brown Algae, Sargassum binderi: Inhibitory Mechanism of iNOS and COX-2 Pathway Interaction

“…The dried SB was ground and sieved through a 50 μm mesh. Polysaccharide extract was prepared by enzymatically hydrolyzing SB powder with Celluclast using a previously reported method [ 30 ], with slight modifications. The SB powder (100 g) was homogenized with 1 L distilled water, and the pH was adjusted to 4.0 using 1 N HCl.…”

Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species

“…Brown seaweed group is the only algae class of Phaeophyceae which contains fucoidan as the cell wall component. Fucoidan is a type of sulfated polysaccharide consisting of sulfated L-fucose with a small portion of xylose, galactose, glucose, mannose, rhamnose and uronic acid (Thinh et al 2013) Fucoidan have extensive bioactivities, such as antioxidants, antiinflammatory, anticoagulant, immuno-stimulatory and antitumor (Li et al 2008;Fernando et al 2020). The properties of fucoidan bioactivity have been the subject of interest of scientists, and various food and fine chemical industries in recent years which drive increasing demand for fucoidan extracted from brown seaweed.…”

Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264.7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation

“…RAW 264.7 macrophages (6 × 10 4 cells/well) were inoculated in 96-well plates and incubated overnight prior to experimental intervention. The cells were then treated with samples of different concentrations for 24 h, added with 10 μL of CCK-8 in each coating, and incubated at 37 °C 5% CO 2 for 2 h [ 62 ]. Optical density was then read at 450 nm using a microplate reader (Thermo Fisher Scientific, Multiskan Sky, Waltham, MA, USA).…”

“…Briefly, 1 μL of Hoechst 33,342 dye (1 mg/mL) was added to each well. The cells were incubated in darkness at 37 °C for 10 min and then washed twice with pre-cooled PBS buffer [ 62 , 63 ]. Red and green fluorescence spots were observed by a fluorescence microscopy (Thermo Fisher Scientific, EVOS M5000, Waltham, Massachusetts, USA) equipped with a DXM1200C colour digital camera (Nikon Corporation, Kumagaya, Saitama, Japan) and processed using NIS-Elements Basic Research v2.2 software.…”

“…Methods of protein extraction and incubation from RAW264.7 macrophages and mouse liver tissue were performed as described [ 62 , 63 , 66 ]. In brief, RAW264.7 macrophages or the liver tissues of mice were lysed and centrifuged at 12,000 rpm for 30 min to remove the fragments and impurities.…”