Efficacy of enzyme-linked immunosorbent assay in serodiagnosis of aspergillosis

Mishra, S. K.; Falkenberg, Shollie M.; Kn, Masihi

doi:10.1128/jcm.17.4.708-710.1983

Cited by 36 publications

(38 citation statements)

References 6 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The earliest study was published in 1983 [22] and the five most current articles were published in 2015 [23], 2016 [24, 25, 26] and 2018 [27]. Nine studies took place in five countries: Japan [25, 28], Brazil [23], United Kingdom [24, 29], France [26, 30, 31] and India [27], but in 4 articles, the study countries were not reported [22, 32, 33, 34].…”

Section: Resultsmentioning

confidence: 99%

“…Nine articles presented DID as the reference test [22, 23, 25, 27, 28, 30, 32, 33, 34]; an article presented two reference tests, DID and CIE [34] and four studies presented only CIE as the reference test [24, 26, 29, 31].…”

Section: Resultsmentioning

confidence: 99%

“…When we performed data extraction, some important differences were observed and deserve to be highlighted. Seven articles performed in-house ELISA tests [22, 23, 28, 30, 32, 33, 34] and six articles described their studies with commercial tests [24, 25, 26, 27, 29, 31]. Different Aspergillus antigens and cut-off points were used in the in-house ELISA tests; the articles that used commercial tests also used several types of antigens and cutoff points included by authors beyond those established by the manufacturer and are described in S1 Table.…”

Section: Resultsmentioning

confidence: 99%

“…During the extraction of ELISA antigen concentration data, five studies with in-house tests presented concentrations varying from 0.1 mcg to 250 mcg per well [22, 23, 30, 33, 34]; in two articles these concentrations were not reported [28, 32].…”

Section: Resultsmentioning

confidence: 99%

“…In the in-house tests, we still find other differences, such as ELISA secondary antibody dilution, with concentrations ranging from 1: 100 to 1: 300 when they were described [22, 23, 33, 34]; in three articles these dilutions were not reported [28, 30, 32]. When we evaluated the cut-off for ELISA, several descriptions were found with titers ranging from 1: 100 to 1: 800; we also found values in OD (optic density), au / mL, in percentage and in absorbance, and there was no comparable value in in-house tests [22, 23, 33, 34]; in three articles, the cut-off was not described [28, 30, 32]. For the ELISA substrate, TMB (3,3′,5,5′-Tetramethylbenzidine) was found in two articles [22, 23], also pNPP (Alkaline Phosphatase Yellow) [33, 34] and OPD (o-Phenylenediamine) [30]; in two articles the substrate was not reported [28, 32].…”

Section: Resultsmentioning

confidence: 99%

See 4 more Smart Citations

Accuracy of serological tests for diagnosis of chronic pulmonary aspergillosis: a systematic review and meta-analysis

Chaves

Oliveira

Venturini

et al. 2019

Preprint

View full text Add to dashboard Cite

22Chronic pulmonary aspergillosis (CPA) is a disease that benefits from cavities as after-effects of 23 tuberculosis, presenting a high mortality rate. Serological tests like double agar gel 24 immunodiffusion test (DID) or the counterimmunoelectrophoresis (CIE) test have been routinely 25 used for CPA diagnosis in the absence of positive cultures; however, they have been replaced by 26 enzyme-linked immunoassay (ELISA), with a variety of methods. 27 This systematic review aims to compare the accuracy of the ELISA test with the reference test 28 (DID and/or CIE) in CPA diagnosis. It was conducted according to the Preferred Reporting Items 29 for Systematic Reviews and Meta-Analyzes (PRISMA). 30 The study was registered in PROSPERO under the registration number CRD42016046057. We 31 searched the electronic databases MEDLINE (PubMed), EMBASE (Elsevier), LILACS (VHL), 32 Cochrane library, and ISI Web of Science. Gray literature was researched in Google Scholars and 33 conference abstracts. We included articles with patients or serum samples from CPA patients who 34 underwent two serological tests: ELISA (index test) and IDD and/or CIE (reference test), using 35 the accuracy of the tests as a result. Original articles were considered without a restriction of date 36 or language. The pooled sensitivity, specificity, and summary receiver operating characteristic 37 curves were estimated. 38 We included 13 studies in the review, but only four studies were included in the meta-analysis. 39 The pooled sensitivities and specificities were 0.93 and 0.97 for the ELISA test. For the reference 40 test (DID and/or CIE), these values were 0.64 and 0.99. Analyses of summary receiver operating 41 characteristic curves yielded 0.99 for ELISA and 0.99 for the reference test (DID and/or CIE). Our 46 Chronic pulmonary aspergillosis (CPA) is a slow and progressive lung disease caused by 47 Aspergillus spp. that develops in preexisting cavities of patients with chronic respiratory diseases, 48 and pulmonary tuberculosis is its main predisposing factor, with a global prevalence estimated at 49 1.2 million cases [1].I Its prognosis is poor, with 38-85% mortality in five years [1,2]. 50CPA presents five clinical forms: 1. aspergillus nodule, 2. pulmonary simple aspergilloma, 51 3. chronic cavitary pulmonary aspergillosis (CCPA), also called complex aspergilloma, 4. chronic 52 fibrosing pulmonary aspergillosis (CFPA), and 5. subacute invasive pulmonary aspergillosis 53 (SAIA) [3]. Aspergilloma is present in only one-third of patients with CPA [1,4]. 54The diagnosis of CPA is based on suggestive images, preferably tomographic images (CT 55 scan), on evidence of microbiological infection by Aspergillus or on the presence of an immune 56 response to this agent, maintained for at least 3 months [3,5]. 57Serologic tests are indispensable for the diagnosis in the absence of positive cultures and 58 are considered the best noninvasive tests to diagnose this entity [6,7]. These tests may be over 90% 59 positive with preci...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Accuracy of serological tests for diagnosis of chronic pulmonary aspergillosis: a systematic review and meta-analysis

Chaves

Oliveira

Venturini

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Comparison of PCR, (1→3)-β-D-glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis

Hashimoto

Yamakami

Kamberi

et al. 1998

J. Clin. Lab. Anal.

View full text Add to dashboard Cite

We compared PCR, galactomannan detection assay using a latex agglutination test and (1→3)-β-D-glucan detection assay in detecting infection in rats experimentally infected with Aspergillus fumigatus. On day 2 after inoculation, (1→3)-β-D-glucan and nested PCR were positive for 80%, while galactomannan detection assay was positive for 60%. In addition, the positive result of nested PCR (87.5%) was higher than those of galactomannan detection assay (75%) and (1→3)β-D-glucan (71.4%) on day 3 after inoculation. The sensitivity of nested PCR was superior to those of galactomannan detection assay and (1→3)-β-D-glucan detection assay. The three diagnostic tests were compared with histopathological findings, and the sensitivity of three diagnostic tests was correlated with histopathological changes. In addition, the elevated levels of (1→3)-β-D-glucan paralleled the development and progression of pulmonary aspergillosis. Our results indicate that a combination of two or three of these tests seems to provide a rapid diagnosis of invasive aspergillosis and assist in the evaluation of the development and severity of invasive aspergillosis.

show abstract

Comparison of PCR, (1→3)‐β‐d‐glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis

Hashimoto

Yamakami

Kamberi

et al. 1998

J. Clin. Lab. Anal.

View full text Add to dashboard Cite

We compared PCR, galactomannan detection assay using a latex agglutination test and (1-->3)-beta-D-glucan detection assay in detecting infection in rats experimentally infected with Aspergillus fumigatus. On day 2 after inoculation, (1-->3)-beta-D-glucan and nested PCR were positive for 80%, while galactomannan detection assay was positive for 60%. In addition, the positive result of nested PCR (87.5%) was higher than those of galactomannan detection assay (75%) and (1-->3)-beta-D-glucan (71.4%) on day 3 after inoculation. The sensitivity of nested PCR was superior to those of galactomannan detection assay and (1-->3)-beta-D-glucan detection assay. The three diagnostic tests were compared with histopathological findings, and the sensitivity of three diagnostic tests was correlated with histopathological changes. In addition, the elevated levels of (1-->3)-beta-D-glucan paralleled the development and progression of pulmonary aspergillosis. Our results indicate that a combination of two or three of these tests seems to provide a rapid diagnosis of invasive aspergillosis and assist in the evaluation of the development and severity of invasive aspergillosis.

show abstract

Efficacy of enzyme-linked immunosorbent assay in serodiagnosis of aspergillosis

Cited by 36 publications

References 6 publications

Accuracy of serological tests for diagnosis of chronic pulmonary aspergillosis: a systematic review and meta-analysis

Accuracy of serological tests for diagnosis of chronic pulmonary aspergillosis: a systematic review and meta-analysis

Comparison of PCR, (1→3)-β-D-glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis

Comparison of PCR, (1→3)‐β‐d‐glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis

Contact Info

Product

Resources

About