Comparison of PCR, (1→3)‐β‐d‐glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis

Hashimoto, A.; Yamakami, Yuriko; Kamberi, Perparim; Yamagata, Eiji; Karashima, Reiko; Nagaoka, Hiroshi; Nasu, Masaru

doi:10.1002/(sici)1098-2825(1998)12:5<257::aid-jcla1>3.3.co;2-3

Cited by 10 publications

(16 citation statements)

References 27 publications

(52 reference statements)

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“…Many groups use different specimen types, with serum rather than EDTA-whole blood often targeted (3,4,9,14,16,29,40,41). The use of serum over EDTA-whole blood allows the same specimen to be tested by both ELISA and PCR and thus provides the opportunity to perform a direct comparison.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Non-Culture-Based Methods for Detection of Systemic Fungal Infections, with an Emphasis on Invasive Candida Infections

2005

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The accepted limitations associated with classic culture techniques for the diagnosis of invasive fungal infections have lead to the emergence of many non-culture-based methods. With superior sensitivities and quicker turnaround times, non-culture-based methods may aid the diagnosis of invasive fungal infections. In this review of the diagnostic service, we assessed the performances of two antigen detection techniques (enzyme-linked immunosorbent assay [ELISA] and latex agglutination) with a molecular method for the detection of invasive Candida infection and invasive aspergillosis. The specificities for all three assays were high (>97%), although the Candida PCR method had enhanced sensitivity over both ELISA and latex agglutination with values of 95%, 75%, and 25%, respectively. However, calculating significant sensitivity values for the Aspergillus detection methods was not feasible due to a low number of proven/probable cases. Despite enhanced sensitivity, the PCR method failed to detect nucleic acid in a probable case of invasive Candida infection that was detected by ELISA. In conclusion, both PCR and ELISA techniques should be used in unison to aid the detection of invasive fungal infections.

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Section: Discussionmentioning

confidence: 99%

Comparison of Non-Culture-Based Methods for Detection of Systemic Fungal Infections, with an Emphasis on Invasive Candida Infections

2005

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“…Elevated plasma levels of 1,3-b-glucan, a fungal cell wall constituent, can be determined with factor G, a horseshoe-crab coagulation enzyme that is extremely sensitive to this polysaccharide. In sera of rats with experimental IA, elevated 1,3-b-glucan levels paralleled the development and progression the disease [28,63]. In a study of 202 febrile episodes in patients aged 2-99 years, the determination of plasma 1,3-b-glucan was found to be a sensitive and specific test for invasive deep mycoses and fungal febrile episodes (Table 3) [66].…”

Section: Serologymentioning

confidence: 99%

Clinical manifestations and diagnosis of invasive aspergillosis in immunocompromised children

Müller

Trusen

Weig

2002

European Journal of Pediatrics

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“…Such tests may provide increased sensitivity compared to antigen detection assays (21,30,33,70). However, most tests to date have been directed to the detection of only one Aspergillus species (12,52,72) or to the detection of only single-copy genes (8,23,29).…”

mentioning

confidence: 99%

Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR-Enzyme Immunoassay

et al. 2004

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We developed a PCR-based assay to differentiate medically important species of Aspergillus from one another and from other opportunistic molds and yeasts by employing universal, fungus-specific primers and DNA probes in an enzyme immunoassay format (PCR-EIA). Oligonucleotide probes, directed to the internal transcribed spacer 2 region of ribosomal DNA from Aspergillus flavus, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus terreus, Aspergillus ustus, and Aspergillus versicolor, differentiated 41 isolates (3 to 9 each of the respective species; P < 0.001) in a PCR-EIA detection matrix and gave no false-positive reactions with 33 species of Acremonium, Exophiala, Candida, Fusarium, Mucor, Paecilomyces, Penicillium, Rhizopus, Scedosporium, Sporothrix, or other aspergilli tested. A single DNA probe to detect all seven of the most medically important Aspergillus species (A. flavus, A. fumigatus, A. nidulans, A. niger, A. terreus, A. ustus, and A. versicolor) was also designed. Identification of Aspergillus species was accomplished within a single day by the PCR-EIA, and as little as 0.5 pg of fungal DNA could be detected by this system. In addition, fungal DNA extracted from tissues of experimentally infected rabbits was successfully amplified and identified using the PCR-EIA system. This method is simple, rapid, and sensitive for the identification of medically important Aspergillus species and for their differentiation from other opportunistic fungi.

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Comparison of PCR, (1→3)‐β‐d‐glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis

Cited by 10 publications

References 27 publications

Comparison of Non-Culture-Based Methods for Detection of Systemic Fungal Infections, with an Emphasis on Invasive Candida Infections

Comparison of Non-Culture-Based Methods for Detection of Systemic Fungal Infections, with an Emphasis on Invasive Candida Infections

Clinical manifestations and diagnosis of invasive aspergillosis in immunocompromised children

Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR-Enzyme Immunoassay

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