1998
DOI: 10.1002/(sici)1098-2825(1998)12:5<257::aid-jcla1>3.3.co;2-3
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Comparison of PCR, (1→3)‐β‐d‐glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis

Abstract: We compared PCR, galactomannan detection assay using a latex agglutination test and (1-->3)-beta-D-glucan detection assay in detecting infection in rats experimentally infected with Aspergillus fumigatus. On day 2 after inoculation, (1-->3)-beta-D-glucan and nested PCR were positive for 80%, while galactomannan detection assay was positive for 60%. In addition, the positive result of nested PCR (87.5%) was higher than those of galactomannan detection assay (75%) and (1-->3)-beta-D-glucan (71.4%) on day 3 after… Show more

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Cited by 10 publications
(16 citation statements)
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“…Many groups use different specimen types, with serum rather than EDTA-whole blood often targeted (3,4,9,14,16,29,40,41). The use of serum over EDTA-whole blood allows the same specimen to be tested by both ELISA and PCR and thus provides the opportunity to perform a direct comparison.…”
Section: Discussionmentioning
confidence: 99%
“…Many groups use different specimen types, with serum rather than EDTA-whole blood often targeted (3,4,9,14,16,29,40,41). The use of serum over EDTA-whole blood allows the same specimen to be tested by both ELISA and PCR and thus provides the opportunity to perform a direct comparison.…”
Section: Discussionmentioning
confidence: 99%
“…Elevated plasma levels of 1,3-b-glucan, a fungal cell wall constituent, can be determined with factor G, a horseshoe-crab coagulation enzyme that is extremely sensitive to this polysaccharide. In sera of rats with experimental IA, elevated 1,3-b-glucan levels paralleled the development and progression the disease [28,63]. In a study of 202 febrile episodes in patients aged 2-99 years, the determination of plasma 1,3-b-glucan was found to be a sensitive and specific test for invasive deep mycoses and fungal febrile episodes (Table 3) [66].…”
Section: Serologymentioning
confidence: 99%
“…Such tests may provide increased sensitivity compared to antigen detection assays (21,30,33,70). However, most tests to date have been directed to the detection of only one Aspergillus species (12,52,72) or to the detection of only single-copy genes (8,23,29).…”
mentioning
confidence: 99%