Efficacy of Cosmetic Materials Using Aronia melanocarpa Leaf Extracts

Abstract

Purpose: To determine the anti-oxidant, whitening, anti-inflammatory and collagen synthesis effects of Aronia melanocarpa (A. melanocarpa) leaf extracts for use as cosmetic ingredients. Methods: Ethanol and hot water leaf extracts of A. melanocarpa were used as test samples. Total polyphenol and flavonoid contents of the leaf extracts were quantified. The anti-oxidant activity of leaf extracts was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The whitening effect of leaf extracts was measured in the mouse melanoma cell line B16F10. The inhibitory effect of leaf extracts on the production of nitric oxide (NO) was measured using lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. The amount of procollagen synthesized with and without leaf extracts was measured in human dermal fibroblasts (HDF) using ELISA. Results: The total polyphenol contents in the ethanol and hot water leaf extracts of A. melanocarpa were 167.46±1.03 μg/mL and 79.04±1.01 μg/mL, respectively, and the total flavonoid contents were 105.15±1.22 μg/mL and 52.21±1.13 μg/mL, respectively. The DPPH radical scavenging effect of ethanol extracts was higher than that of hot water extracts. In the mouse melanoma cell line B16F10, the tyrosinase inhibitory activity and melanin inhibition rate using 100 μg/mL ethanol extracts and 100 μg/mL hot water extracts were 21% and 24%, 18% and 18%, respectively. The tyrosinase inhibitory activity and melanin inhibition induced by α-melanocytestimulating hormone (α-MSH) were higher in ethanol extracts than in hot water extracts. The anti-inflammatory effect of ethanol extracts, measured as the inhibition of NO production in LPS-stimulated RAW 264.7 mouse macrophages, was higher than that of hot water extracts. Procollagen synthesis was lower than the positive control group due to the respective 58% and 41% increases in procollagen synthesis with the concentration of 200 μg/mL. Conclusion: Data showed that A. melanocarpa leaf extracts isolated using ethanol exhibited higher anti-oxidant, whitening, antiinflammatory, and collagen synthesis effects than those isolated using hot water. Thus, ethanol leaf extracts can be considered as a candidate for use as ingredients in cosmetic products.

Biocosmetics: technological advances and future outlook

“…Jerusalem artichoke extract or powder fermented with P. rhodozyma for astaxanthin synthesis was studied because of its anti-inflammatory, sun proofing, antiaging, antioxidative, and immune-boosting functions (Jiang et al 2017). Aronia melanocarpa leaf and fruit extract are used in cosmetic products for skin conditioning, skin whitening, anti-inflammatory, and collagen-boosting properties (Cujic et al 2017;Lee and Ryu 2018). Novel cosmeceutical delivery systems that act as smart carriers for encapsulation or attachment of therapeutic ingredients need to be explored.…”

Effects of Soybean Diet and Back Massage on the Neck Skin of Middle-Aged Women

“…http://dx.doi.org/10.20402/ajbc.2018.0247 natto)를 이용하는 것이 상업효소를 이용하는 것보다 가격 경쟁 력이나 안전성 면에서 우수하여 복합 기능성 화장품 원료로 사 용 가능함을 시사하였다 (Moon et al, 2015). 현대의 급변하는 다양한 변화 속에 노령화 사회 가속화는 자 신들의 건강과 외적인 미에 대한 관심을 증가시키고 있으며 (Lee & Ryu, 2018), 아름답게 오래 살기 위한 다양한 변화들도 시도 되고 있다. 그 중 등 마사지는 혈액과 림프순환을 원활하게 하 여 영양 및 산소 공급을 촉진시켜 인체의 대사활동을 높이고 심 신의 안정을 도와 피부개선 및 비만에도 효과가 있다고 하였다 (Yun et al, 2011 In the Cont group, the sebum level increased by 19% from 7.38±1.60 before the experiment to 8.80±1.71 after 12 weeks ( ** p<0.01).…”