Efficacy of CNC-diode laser combine with chlorophylls to eliminate staphylococcus aureus biofilm

Astuti, Suryani Dyah; Arifianto, Deni; Drantantiyas, Nike Dwi Grevika; Nasution, Aulia; Abdurachman,

doi:10.1109/issimm.2016.7803722

Cited by 20 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The success of photoinactivation is determined by the type and concentration of photosensitizer as well as the output power and duration of exposure to light. 14 The maximum absorption spectrum of photosensitizer molecules is a point of reference in selecting the right wavelength of light to allow an optimum absorption rate. The amount of light photon energy absorbed by a photosensitizer molecule will determine the length of the molecular excitation stage at the triplet level and the chance of the reaction with the triplet oxygen molecule.…”

Section: Introductionmentioning

confidence: 99%

“…The structure of chlorophyll molecule as same as the porphyrin that the first generation photosensitizer. 14,17 Chlorophyll pigment has a long triplet excitation stage (≤10 -8 seconds) so that the odds of more chemical reaction with oxygen molecules. 14,15 Chlorophyll extract has been used in photoinactivation to Staphylococcus aureus 13 and Streptococcus mutant 18 of pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…14,17 Chlorophyll pigment has a long triplet excitation stage (≤10 -8 seconds) so that the odds of more chemical reaction with oxygen molecules. 14,15 Chlorophyll extract has been used in photoinactivation to Staphylococcus aureus 13 and Streptococcus mutant 18 of pathogenic bacteria. In the country of Indonesia are available many green plants that have high chlorophyll content such as Papaya and Suji.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

et al. 2019

View full text Add to dashboard Cite

Introduction: Photodynamic inactivation has been developed to kill pathogenic microbes. In addition, some techniques have been introduced to minimize the biofilm resistance to antifungal properties in inhibiting cell growth. The principle of photodynamic inactivation different to antifungal drugs therapy which is resistant to biofilms. The presence of reactive oxygen species (ROS) that generating in photodynamic inactivation mechanisms can be damaging of biofilm cells and the principle of light transmission that could be penetrating in matrix layers of extracellular polymeric substance (EPS) until reaching the target cells at the base layers of biofilm. The present work aims to explore the potential of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser. The potential of chlorophyll photosensitizer was evaluated based on the efficacy of inactivation C. albicans biofilm cell through a cell viability test and an organic compound test. Methods: The treatment of photoinactivation was administered to 12 groups of C. albicans biofilm for four days using the 445 nm laser and the 650 nm laser. The 445 nm and 650 nm lasers activated the chlorophyll extract of the papaya leaf (0.5 mg/L) at the same energy density. The energy density variation was determined as 5, 10, 20, 30 and 40 J/cm2 with the duration of exposure of each laser adjusted to the absorbance percentage of chlorophyll extract of the papaya leaf. Results: The absorbance percentage of chlorophyll extracts of the papaya leaf on wavelengths of 650 nm and 445 nm respectively were 22.26% and 60.29%, respectively. The most effective treated group was a group of the laser with the addition of chlorophyll, done by the 650 nm lasers with inactivation about 32% (P=0.001), while the 445 nm lasers only 25% (P=0.061). The maximum malondialdehyde levels by treatment of the laser 650 nm were (0.046±0.004) nmol/mg. Conclusion: The use of chlorophyll extract of the papaya leaf as a photosensitizer, resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation up to 32% (with chlorophyll) and 25% (without chlorophyll). The utilization of chlorophyll extract of the papaya leaf would increase the antifungal effects with the activation by the diode laser in the biofilm of C. albicans

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

et al. 2019

View full text Add to dashboard Cite

show abstract

“…One of the laser interactions with tissue is the photochemical effect. This laser property is used for various applications in the medical field [15,16]. Recent research studies have mentioned laser as an alternative treatment of antimicrobial, antifungal, and photobiomodulation that is relatively inexpensive and has no negative effects [17–20].…”

Section: Introductionmentioning

confidence: 99%

The effects of laser diode treatment on liver dysfunction of Mus musculus due to carbofuran exposure: An in vivo study

Astuti¹,

Victory²,

Mahmud³

et al. 2019

J Adv Vet Anim Res

View full text Add to dashboard Cite

Objective:The aim of this study is to determine the effect of laser diode as an alternative treatment on liver dysfunction (in vivo study) that is caused by carbofuran using male mice (Mus musculus) strain Balb/C.Materials and Methods:The samples were divided into three groups, namely, Group C–L– (control group, no treatment), Group C+L– (only treated by carbofuran treatment), and Group C+L+ (treatment group, treated by carbofuran and laser-puncture) with five replications each. After being treated, each liver slice of samples was observed using microscope to get the histology result and then scored.Results:Carbofuran contamination can lead to inflammation of cells and necrosis. The histology results and the scoring test showed that the liver cells repair with the energy dose of laser diode at 0.5 and 1.0 Joule.Conclusion:The optimum energy dose in this study was 1.0 Joule which had the closest score of inflammatory cells and necrosis to normal liver cells.

show abstract

“…Tese expensive treatments can also result in anorexia, headaches, nausea, and dizziness in some people [2,3]. Acupuncture and electric needles have both been used in research for the invasive diagnosis and therapy of organ disease [4][5][6], and noninvasively using light [7][8][9]. Te human body is equipped with an electrical system [10].…”

Section: Introductionmentioning

confidence: 99%

Ameliorative and Renoprotective Effect of Electrical Stimulation on Blood Sugar, Blood Urea Nitrogen (BUN), Creatinine Levels, and the Islets of Langerhans Weight in Diabetic Mice

Suhariningsih

Glory

Khaleyla

et al. 2022

Veterinary Medicine International

View full text Add to dashboard Cite

Diabetes mellitus (DM) is a chronic metabolic disease or disorder characterized by high blood sugar levels as well as impaired carbohydrate, lipid, and protein metabolism due to insulin function insufficiency. Insulin deficiency can be caused by impaired or deficient insulin production by Langerhans beta cells in the pancreas or by a lack of responsiveness of the body's cells to insulin. This study aims to the effects of electrostimulation on the ameliorative (improves disease manifestations) or renoprotective (protects the kidneys) in a diabetic rat model using noninvasive (electrical stimulation with the magnetic and nonmagnetic electrode) and invasive (using needles) methods. This study used 25 female rats, with a normal control group (KN), a diabetes control group (KD), a needle treatment group (A), an electro-stimulator treatment group with a magnetic electrode (M), and an ES group with a nonmagnetic electrode (ES) (L). The electro-stimulator used AES-05 with a magnetic field strength of 90 mT at two acupoints, Pishu (BL20) and Shenshu (BL23). The treatment was administered 12 times in one month with a therapy time of 6.6 minutes per session. Body weight and blood sugar levels were compared before and after the treatment. After treatment, the diameter of the islets of Langerhans, as well as levels of creatinine and blood urea nitrogen (BUN), was measured. Furthermore, statistical analysis was performed (α = 0.05). The results of this study showed that electrical stimulation treatments with needle-invasive, noninvasive magnetic electrodes, and nonmagnetic electrodes significantly reduced diabetic rats’ blood glucose levels before and after the treatment. The analysis of the diameter of the islets of Langerhans revealed a significant difference between the treatment groups. The analysis of creatinine levels revealed a significant difference between groups, but creatinine levels in the group with the magnetic electrode (0.58 ± 0.17 mg/dL) were not significantly different from the control group (0.58 ± 0.07 mg/dL). The BUN test results revealed a significant difference compared with the diabetic control group, but no significant difference with the magnetic electrode treatment group. Conclusion. Based on the results, the most effective therapy for diabetes is a noninvasive method with magnetic (M) electrodes.

show abstract

Efficacy of CNC-diode laser combine with chlorophylls to eliminate staphylococcus aureus biofilm

Cited by 20 publications

References 18 publications

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

The effects of laser diode treatment on liver dysfunction of Mus musculus due to carbofuran exposure: An in vivo study

Ameliorative and Renoprotective Effect of Electrical Stimulation on Blood Sugar, Blood Urea Nitrogen (BUN), Creatinine Levels, and the Islets of Langerhans Weight in Diabetic Mice

Contact Info

Product

Resources

About