Efficacy of Bacterial Biocontrol Agents and Environmental-Friendly Fine Chemicals for Suppression of Ganoderma Boninense Growth

Abstract

Abstract. Various means are available to control Ganoderma boninense, however none of those methods are efficacious. Biological control agents method is one of promising way to control the pathogen, that has eco-friendly-mannered. The objective of this research was to evaluate the effectiveness of bacterial strains and the application of some environmentally friendly fine chemicals including ammonium, calcium, and salicylic acid in suppressing of mycelia growth of G. boninense. In vitro results showed that among eight strains, EB13, as identified as B. amyloliquefaciens, has reduced G. boninense growth diameter significantly (P<0.05%) at the highest percentage (81%). It followed by MB31, MB72, MB58 and MB41 74%, 74%,71% and 63%, respectively. Conversely MB20, EB45 and EB53 did not suppress the fungal pathogen growth. The chemical control test indicated that salicylic acid has inhibited the growth of the pathogen significantly at the highest percentage of 52% (P<0.05%). The lowest growth inhibition was by ammonium nitrate (2%). On the contrary, calcium chloride could not inhibit the G. boninense growth. Further assessment on pH in PDA media containing of 200mM ammonium was conducted in order to increase the inhibition activity of the ammonium nitrate. The result exhibited that the medium containing ammonium nitrate with pH 8.5 has reduced G. boninense growth significantly at the highest percentage (53%).It followed by pH 8.0 and pH 7.5 at 10% and 2%, respectively.

The Potency of Bacillus siamensis LDR as Biocontrol Agent Against Fungal Phytopathogen

“…can inhibit 54.2% growth of Ganoderma boninense [3]. Yuliar and Suciatmih [15] reported B. amyloliquefaciens EB13 have antagonistic activity against Ganoderma boninense. Furthermore, Khan et al [1] stated Bacillus species was potential to become potential biocontrol against Fusarium.…”

Physico-Chemical, Viability Evaluations and Efficacy Assessment of Bacillus subtilis Against Soft Rot Disease in Phalaenopsis

“…Disease intensity was observed weekly until 4 months after inoculation. The degree of antagonist colonization on the leaves and root zones were measured according to Yuliar & Suciatmih (2018). All the data gathered were analyzed using ANOVA continued by DMRT at 95% level of confidence.…”