Effects on the osseointegration of titanium implants incorporating calcium–magnesium: a resonance frequency and histomorphometric analysis in rabbit tibia

Gehrke, Sérgio Alexandre; Val, José Eduardo Maté Sánchez de; Domínguez, Manuel Fernández; Aza, Piedad N. De; Moreno, Gerardo Gómez; Calvo-Guirado, José Luís

doi:10.1111/clr.12909

Cited by 16 publications

(20 citation statements)

References 24 publications

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“…Several investigations to improve or accelerate the process of osseointegration have been studied, as well worked on to elaborate new treatments for the surface of the implants (micro topography) with different physical and chemical characteristics [5][6][7][8][9]. These modifications have shown good results, mainly in pre-clinical studies, as reported in the literature [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

et al. 2019

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A new implant design with healing chambers in the threads was analyzed and compared with a conventional implant macrogeometry, both implants models with and without surface treatment. Eighty conical implants were prepared using commercially pure titanium (grade IV) by the company Implacil De Bortoli (São Paulo, Brazil). Four groups were performed, as described below: Group 1 (G1), traditional conical implants with surface treatment; group 2 (G2), traditional conical implants without surface treatment (machined surface); group 3 (G3), new conical implant design with surface treatment; group 4 (G4), new conical implant design without surface treatment. The implants were placed in the two tibias (n = 2 implants per tibia) of twenty New Zealand rabbits determined by randomization. The animals were euthanized after 15 days (Time 1) and 30 days (Time 2). The parameters evaluated were the implant stability quotient (ISQ), removal torque values (RTv), and histomorphometric evaluation to determine the bone to implant contact (%BIC) and bone area fraction occupancy (BAFO%). The results showed that the implants with the macrogeometry modified with healing chambers in the threads produced a significant enhancement in the osseointegration, accelerating this process. The statistical analyses of ISQ and RTv showed a significative statistical difference between the groups in both time periods of evaluation (p ≤ 0.0001). Moreover, an important increase in the histological parameters were found for groups G3 and G4, with significant statistical differences to the BIC% (in the Time 1 p = 0.0406 and in the Time 2 p < 0.0001) and the BAFO% ((in the Time 1 p = 0.0002 and in the Time 2 p = 0.0045). In conclusion, the result data showed that the implants with the new macrogeometry, presenting the healing chambers in the threads, produced a significant enhancement in the osseointegration, accelerating the process.

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Section: Introductionmentioning

confidence: 99%

New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

et al. 2019

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“…Among these surfaces are those coated with nanoparticles and calcium ions [24,25,26]. The incorporation of calcium ions on the surface of a dental implant can in fact stimulate cell attachment, proliferation, alkaline phosphatase activity, and can upregulate gene expression of bone-related proteins [24,25,26].…”

Section: Introductionmentioning

confidence: 99%

Scanning Electron Microscope (SEM) Evaluation of the Interface between a Nanostructured Calcium-Incorporated Dental Implant Surface and the Human Bone

et al. 2017

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Purpose. The aim of this scanning electron microscope (SEM) study was to investigate the interface between the bone and a novel nanostructured calcium-incorporated dental implant surface in humans. Methods. A dental implant (Anyridge®, Megagen Implant Co., Gyeongbuk, South Korea) with a nanostructured calcium-incorporated surface (Xpeed®, Megagen Implant Co., Gyeongbuk, South Korea), which had been placed a month earlier in a fully healed site of the posterior maxilla (#14) of a 48-year-old female patient, and which had been subjected to immediate functional loading, was removed after a traumatic injury. Despite the violent trauma that caused mobilization of the fixture, its surface appeared to be covered by a firmly attached, intact tissue; therefore, it was subjected to SEM examination. The implant surface of an unused nanostructured calcium-incorporated implant was also observed under SEM, as control. Results. The surface of the unused implant showed a highly-structured texture, carved by irregular, multi-scale hollows reminiscent of a fractal structure. It appeared perfectly clean and devoid of any contamination. The human specimen showed trabecular bone firmly anchored to the implant surface, bridging the screw threads and filling the spaces among them. Conclusions. Within the limits of this human histological report, the sample analyzed showed that the nanostructured calcium-incorporated surface was covered by new bone, one month after placement in the posterior maxilla, under an immediate functional loading protocol.

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“…Firstly, surface treatment with inorganic ions induces a significant roughness modification which is responsible, in turn, of an increased cell adhesion and of a reduced osteoblasts cytotoxicity, as demonstrated in our previous paper 9) . Furthermore, it can be argued that magnesium and calcium addition on test surface plays a pivotal role in promoting and enhancing cell differentiation thus improving bone-implant contact, as supported by the scientific literature 21,22,24) . However the reasons of this outcome still remain unknown and need to be clarified in future studies.…”

Section: Discussionmentioning

confidence: 92%

“…A morphological study of the titanium discs, performed by AFM, reported in our previous work 9) , evidences a nanometric modification of the surface. The innovation of this surface is represented by the surface treatment which create a stable layer on titanium discs able to better promote cell adhesion, as firstly demonstrated by Zizzari et al 9) ,and subsequently by Gehrke et al 24) . In the present work, the goal was to better understand if this surface treatment could also have a positive influence on the differentiation of mesenchymal stem cells towards the osteoblastic lineage.…”

Section: Discussionmentioning

confidence: 99%

“…The CaMg layer of the Test Group was obtained by blasting the samples with 30-80 µm CaMg microparticles, supplied as composite, at a pressure of 2.5 Mpa with a distance of 5 cm, in a decontaminated ambient (apparatus) with sterilized air. Each sample was prepared, packaged, and sterilized using the same requirements and care for implant packing 24) . The discs were used for in vitro experiments, for which they were aseptically transferred to sterile 48-well cell-culture trays and exposed to UV radiation for 1 h.…”

Section: Sample Preparationmentioning

confidence: 99%

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Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

et al. 2018

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Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite, (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.

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Effects on the osseointegration of titanium implants incorporating calcium–magnesium: a resonance frequency and histomorphometric analysis in rabbit tibia

Abstract: Within the limitations of this study, the results indicate that the deposition of CaMg on the SLA titanium surface may be effective in enhancing the osseointegration of moderately rough grit-blasted implants by increasing the degree of bone-implant contact.

Cited by 16 publications

References 24 publications

New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

Scanning Electron Microscope (SEM) Evaluation of the Interface between a Nanostructured Calcium-Incorporated Dental Implant Surface and the Human Bone

Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

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