Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

Abstract

Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite, (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.

Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

“…Several studies demonstrated that surface modifications in dental implants are able to further ameliorate their integration process into the host bone tissue. In particular, a previous study performed in our laboratory demonstrated that a sandblasted and acid-etched surface further treated with inorganic ions guarantees an improvement in cell viability, a low inflammatory response, a faster promotion of the osteoblastic differentiation, a higher biocompatibility, and a better tolerability by both human osteoblasts and DPSCs, compared to a simply sandblasted and acid-etched titanium surface [6,7]. For this reason, we decided to compare, in the present study, only machined, i.e.…”

“…Only impacted teeth without dental pathologies were included in the study. After the extraction, all the remaining soft tissues were mechanically removed and processed, as reported in our previous work [7].…”

“…The dental pulp was washed with phosphate-buffered saline (PBS), kept in Minimum Essential Medium Eagle, alpha Modification (α-MEM, Sigma-Aldrich, St. Louis, MO, US) with the addition of 10% of Foetal Bovine Serum (FBS) and 1% of penicillin/streptavidin (EuroClone S.p.A, Milan, Italy), and delivered to the laboratory for stem cells isolation [7]. When cell culture reached sub-confluence (80-90% of surface area), they were subcultured.…”

“…When cell culture reached sub-confluence (80-90% of surface area), they were subcultured. Antigen expression of CD29, CD45, CD105, CD73 CD90, and SSEA-4 was checked by flow cytometry [7].…”

Adhesion, integration and osteogenesis of human dental pulp stem cells on biomimetic implant surfaces combined with plasma derived products

“…When hDPSCs are seeded on porous surfaces they show osteoblast differentiation, production of appreciable amounts of bone morphogenetic proteins as well as vascular endothelial growth factor and specific bone proteins (Perrotti et al, 2013). Recent work suggests that chemically modified microrough titanium surfaces are more osteoinductive, which represents a significant advantage for application in the dental clinic (Boyan et al, 2018;DE Colli et al, 2018). In the present study a small but significant difference in the levels of expression of OSTERIX was found, when comparing Ti6Al4V (smooth) and BAS (rough biomimetic) surface-grown hDPSCs in control (no osteoblastic stimulation) conditions.…”