Effects on Pre-treatment and Different Tissue Culture Media for Androgenesis in Stevia rebaudiana Bertoni

Uskutoğlu, Tansu; Uskutoğlu, Duygu; Turgut, Kenan

doi:10.1007/s12355-019-00722-z

Cited by 7 publications

(2 citation statements)

References 29 publications

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“…Currently, stevia is consumed in Japan, Brazil, Korea, Israel, the United States, Argentina, China, Canada, Paraguay, and Indonesia (Crammer and Ikan 1986;Singh and Rao 2005;Yadav et al 2011) and no adverse effects have been reported from its use to date (Kinghorn and Soejarto 1985;Brandle and Rosa 1992;Yadav et al 2011). Stevia was used for the first time in Japan for the food and pharmaceutical industry and its use is increasing day by day (Uskutoğlu et al 2019). It is also used as a natural sweetener in many countries such as North and South America, Southern Europe, Korea, Thailand, China, India and Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine

Akbaş,

Turgut

2023

IJIASR

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The primary objective of this study was to establish a protocol for induction of polyploidy in S. rebaudiana var. Levent 93. As a plant material in vitro propagated seedlings of stevia variety Levent 93 were used. For induction of polyploidy, shoot tips with 3-4 leaves of seedlings were immersed in 0.5% concentration of colchicine solution for 1, 2 and 4 hours. Then, shoot tips treated with colchicine solution and distilled water were taken into rooting medium. Plants that developed successfully in the rooting medium were transferred to pots and acclimatized to the external environment. Flow cytometry ploidy analysis was performed on plants treated with colchicine solution after approximately 8 months. According to the ploidy analysis results, it was determined that 17 plants were diploid and 3 plants were tetraploid among 20 plants that survived after treatment with colchicine. All tetraploid plants were observed in 2 hours treatments. As a result of the study, the viability rate, plant height, number of leaves, number of branches, chlorophyll amount, ploidy levels and core DNA contents of stevia plants treated with colchicine were determined and diploid and tetraploid plants were compared. When diploid plants and tetraploid plants are compared, it is concluded that tetraploid plants have smaller averages in terms of plant height average, while they have larger averages in terms of leaf number average and number of branches.

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Section: Introductionmentioning

confidence: 99%

Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine

Akbaş,

Turgut

2023

IJIASR

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“…It is widely accepted how vacuolate microspores and young bi-cellular pollen grains are more sensitive to the androgenic induction ( Maheshwari et al, 1980 ; Dunwell, 2010 ; Dwivedi et al, 2015 ; Canonge et al, 2020 ). On the other hand, it has been demonstrated in different species how microspore and pollen stage of development can be correlated with some features of the flower, as is the case of bud length, pedicel length, anther length and petal to anther ratio in Brassica napus L. ( Pechan and Keller, 1988 ), bud length and perianth morphological markers in Solanum lycopersicum L. ( Brukhin et al, 2003 ), pigmentation degree of anthers ( Kim et al, 2004 ) and calyx-corolla ratio ( Bárány et al, 2005 ) in Capsicum annuum L., or more recently, flower bud size in Stevia rebaudiana Bertoni ( Uskutoǧlu et al, 2019 ), and bud length, anther color, and filament length in Opuntia ficus-indica L. Mill ( Bouamama-Gzara et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis

Galán-Ávila¹,

García‐Fortea

Prohens

et al. 2021

Front. Plant Sci.

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Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We have studied the whole process of anther and pollen grain formation during meiosis, microsporogenesis, and microgametogenesis and correlated the different microgametophyte developmental stages with bud length in plants from varieties USO31 and Finola. We also studied microspore and pollen amyloplast content and studied the effect of a cold pretreatment to excised buds prior to microspore in vitro culture. Up to 476,903 microspores and pollen grains per male flower, with in vivo microspore viability rates from 53.71 to 70.88% were found. A high uniformity in the developmental stage of microspores and pollen grains contained in anthers was observed, and this allowed the identification of bud length intervals containing mostly vacuolate microspores and young bi-cellular pollen grains. The starch presence in C. sativa microspores and pollen grains follows a similar pattern to that observed in species recalcitrant to androgenesis. Although at a low frequency, cold-shock pretreatment applied on buds can deviate the naturally occurring gametophytic pathway toward an embryogenic development. This represents the first report concerning androgenesis induction in C. sativa, which lays the foundations for double haploid research in this species.

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Species with Haploid or Doubled Haploid Protocols

Seguí-Simarro

Moreno

Fernández

et al. 2021

Methods in Molecular Biology

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Effects on Pre-treatment and Different Tissue Culture Media for Androgenesis in Stevia rebaudiana Bertoni

Cited by 7 publications

References 29 publications

Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine

Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine

Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis

Species with Haploid or Doubled Haploid Protocols

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