Species with Haploid or Doubled Haploid Protocols

Seguí-Simarro, José M.; Moreno, José David; Fernández, Marina Guillot; Mir, Ricardo

doi:10.1007/978-1-0716-1315-3_3

Cited by 11 publications

(5 citation statements)

References 771 publications

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“…Haploid plants can be obtained spontaneously through parthenogenesis, semigamy or polyembryony, but the frequency of haploid is very low (Dunwell, 2010;Asadi & Seguí-Simarro, 2021;Seguí-Simarro et al, 2021b;Badulescu et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

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Androgenesis in tomato (Solanum lycopersicum L.)-Effect of genotypes, microspore development stage, pre-treatments and media composition on induction of haploids

Kambale

Gnanam

Balasubramanian

et al. 2023

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Doubled haploid (DH) technology remarkably accelerates the crop breeding by obtaining homozygous lines in a single generation. The present study was targeted in generating haploid plants through androgenesis. Anthers from immature flower buds of six tomato genotypes viz., LE-1230, LE-1236, LE-1256 TLCV 2, PKM 1 and TNAU tomato hybrid CO 3 were used for induction of haploids. A preliminary study based on callus induction frequency (CIF), more than 5% was helpful in short listing flower bud size, pre-treatments and growth regulator combinations. Subsequently, anthers from two different sized flower buds (4 and 6 mm length), dissected either from fresh or pre-treated flower buds (2 and 5 days in dark at 4 °C or gamma irradiated) were inoculated in MS medium fortified with different growth regulators for callus induction. Among the genotypes, TLCV 2 had recorded the maximum CIF (38.80%) from anthers of 4 mm long flower buds followed by TNAU tomato hybrid CO 3 (34.70%). Throughout the study, anthers from 4 mm long flower buds responded the best for callus induction. Among the pre-treatments, anthers from gamma irradiated flower buds recorded the highest CIF (31.90%) when compared to others. Cold shock (4 °C) in dark to flower buds for 2 days had improved the CIF of anthers when compared to fresh in LE 1230, LE 1238, TLCV2 and TNAU tomato hybrid CO 3, but when the cold shock was increased to 5 days, invariably there was a reduction in CIF in all the six genotypes. TA 8 (MS + 2iP (0.5 mg L-1) + NAA (0.5 mg L-1)) medium was found to be the best for maximum CIF in LE 1230 and PKM1, TA1 (MS + 2iP (1.0 mg L-1) + IAA (2.0 mg L-1)) in LE 1238, LE 1256 and TNAU tomato hybrid CO 3 and TA7 (MS + 2iP (0.5 mg L-1) + Kinetin (1.5 mg L-1) + NAA (1.0 mg L-1)) for TLCV 2 genotypes. The callus induced was sub cultured at monthly intervals in the same medium for proliferation and later transferred to regeneration medium. A good number of shoots got regenerated only from anther calli of TNAU hybrid CO 3 that was sub cultured in MS medium fortified with Zeatin (0.5 mg L-1). The clumps of shoots induced were separated and inoculated in MS medium supplemented with GA3 (0.5 mg L-1) for shoot elongation. After 4-6 weeks, the elongated shoots were transferred to half strength MS medium enhanced with IBA (1 mg L-1). Profuse rooting from the base of the shoot was noticed in 4-5 weeks. The stomatal count with leaves from the diploid plants and in vitro plants observed were 3-4 and 1 respectively indicating the haploidy nature of in vitro plants.

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Section: Discussionmentioning

confidence: 99%

“…Haploid production through androgenesis and gynogenesis are the most important in vitro breeding techniques used in modern breeding programmes (Don Palmer & Keller, 2005;Weyen, 2021). Pure lines (doubled haploid plants) can be obtained in a short time by using in vitro anther culture (Seguí-Simarro et al, 2021b).…”

Section: Discussionmentioning

confidence: 99%

Androgenesis in tomato (Solanum lycopersicum L.)-Effect of genotypes, microspore development stage, pre-treatments and media composition on induction of haploids

Kambale

Gnanam

Balasubramanian

et al. 2023

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“…Techniques for generating DH have been applied to nearly 400 species so far, resulting in the global introduction of more than 300 DH-based varieties across 12 different species (Seguı-Simarro et al, 2021;Weyen, 2021). With its speed, efficiency, genetic stability, and trait evaluation benefits, DH breeding can be a favoured method Dubey et al 10.3389/fpls.2024.1394413 in commercial IC breeding programs, facilitating the timely delivery of improved varieties to meet the needs of farmers and consumers.…”

Section: Breeding Schemesmentioning

confidence: 99%

Harnessing monocrop breeding strategies for intercrops

Dubey,

Zustovi,

Landschoot

et al. 2024

Front. Plant Sci.

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Intercropping is considered advantageous for many reasons, including increased yield stability, nutritional value and the provision of various regulating ecosystem services. However, intercropping also introduces diverse competition effects between the mixing partners, which can negatively impact their agronomic performance. Therefore, selecting complementary intercropping partners is the key to realizing a well-mixed crop production. Several specialized intercrop breeding concepts have been proposed to support the development of complementary varieties, but their practical implementation still needs to be improved. To lower this adoption threshold, we explore the potential of introducing minor adaptations to commonly used monocrop breeding strategies as an initial stepping stone towards implementing dedicated intercrop breeding schemes. While we acknowledge that recurrent selection for reciprocal mixing abilities is likely a more effective breeding paradigm to obtain genetic progress for intercrops, a well-considered adaptation of monoculture breeding strategies is far less intrusive concerning the design of the breeding programme and allows for balancing genetic gain for both monocrop and intercrop performance. The main idea is to develop compatible variety combinations by improving the monocrop performance in the two breeding pools in parallel and testing for intercrop performance in the later stages of selection. We show that the optimal stage for switching from monocrop to intercrop testing should be adapted to the specificity of the crop and the heritability of the traits involved. However, the genetic correlation between the monocrop and intercrop trait performance is the primary driver of the intercrop breeding scheme optimization process.

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“…During in vitro microspore embryogenesis, vacuolated microspores or young pollen grains are induced to become haploid embryos, which can be converted to homozygous doubled haploid (DH) plants after chromosome doubling. DH technology produces 100% homozygous plants in just one generation (Seguí‐Simarro et al, 2021b) and has been applied in nearly 400 different plant species (Seguí‐Simarro et al, 2021a) to accelerate the plant breeding programs (Weyen, 2021). This process is also a fascinating example of plant cell totipotency.…”

Section: Introductionmentioning

confidence: 99%

The different response of Brassica napus genotypes to microspore embryogenesis induced by heat shock and trichostatin A is not determined by changes in cell wall structure and composition but by different stress tolerance

Camacho‐Fernández,

Corral‐Martínez,

Calabuig‐Serna

et al. 2024

Physiologia Plantarum

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During microspore embryogenesis, microspores are induced to develop into haploid embryos. In Brassica napus, microspore embryogenesis is induced by a heat shock (HS), which initially produces embryogenic structures with different cell wall architectures and compositions, and with different potentials to develop into embryos. The B. napus DH4079 and DH12075 genotypes have high and very low embryo yields, respectively. In DH12075, embryo yield is greatly increased by combining HS and the histone deacetylase (HDAC) inhibitor trichostatin A (TSA). However, we show that HS + TSA inhibits embryogenesis in the highly embryogenic DH4079 line. To ascertain why TSA has such different effects in these lines, we treated DH4079 and DH12075 microspore cultures with TSA and compared the cell wall structure and composition of the different embryogenic structures in both lines, specifically the in situ levels and distribution of callose, cellulose, arabinogalactan proteins and high and low methyl‐esterified pectin. For both lines, HS + TSA led to the formation of cell walls unfavorable for embryogenesis progression, with reduced levels of arabinogalactan proteins, reduced cell adhesion of inner walls and altered pectin composition. Thus, TSA effects on cell walls cannot explain their different embryogenic response to TSA. We also applied TSA to DH4079 cultures at different times and concentrations before HS application, with no negative effects on embryogenic induction. These results indicate that DH4079 microspores are hypersensitive to combined TSA and HS treatments, and open up new hypotheses about the causes of such hypersensitivity.

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Species with Haploid or Doubled Haploid Protocols

Cited by 11 publications

References 771 publications

Androgenesis in tomato (Solanum lycopersicum L.)-Effect of genotypes, microspore development stage, pre-treatments and media composition on induction of haploids

Androgenesis in tomato (Solanum lycopersicum L.)-Effect of genotypes, microspore development stage, pre-treatments and media composition on induction of haploids

Harnessing monocrop breeding strategies for intercrops

The different response of Brassica napus genotypes to microspore embryogenesis induced by heat shock and trichostatin A is not determined by changes in cell wall structure and composition but by different stress tolerance

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