Effects of yeast culture and fibrolytic enzyme supplementation on in vitro fermentation characteristics of low-quality cereal straws1

Abstract

The effects of yeast culture and fibrolytic enzyme preparation (containing cellulase and xylanase) on in vitro fermentation characteristics of rice straw, wheat straw, maize stover, and maize stover silage were examined using an in vitro gas production technique. Four levels of yeast culture and fibrolytic enzyme supplements (0, 2.5, 5.0, and 7.5 g/kg of straw DM, respectively) were tested in a 4 x 4 factorial arrangement. Supplementation of yeast culture increased the cumulative gas production, theoretical maximum of gas production, rate of gas production, IVDMD, and in vitro OM disappearance (IVOMD), and decreased the lag time for each type of straw. Fibrolytic enzyme supplementation tended to increase cumulative gas production, theoretical maximum of gas production, and rate of gas production; prolonged lag time of gas production; and enhanced IVDMD and IVOMD for 4 types of cereal straws, with the significance of this effect being dependent on the level of supplemented enzymes. There were significant interactions between fibrolytic enzymes and yeast on all in vitro gas production parameters, IVDMD, and IVOMD of each type of straw. The outcome of this research indicated that the application of fibrolytic enzyme preparation and yeast culture could improve in vitro gas production fermentation of cereal straws.

Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios

“…Nsereko et al (6) suggested that exogenous enzymes could increase fiber degradation through a hydrolytic action resulting in more effective rumen fermentation. Tang et al (30) suggested that in vitro GP variables usually reflect the characteristics of the fermentation process. In the present study, the interactive effects for in vitro GP parameters suggest that it is important to identify appropriate fibrolytic enzyme administration and rations with ratio of maize silage to concentrate.…”

Improving Sugarcane Bagasse as Animal Feed by Ammoniation and Followed by Fermentation with Trichoderma harzianum (In Vitro Study)

“…However, agricultural and agro-industrial by product feed conteined high percentage of fiber and difficult to be digested by animals (Suwandyastuti and Rimbawanto, 2011). Researchs conducted by scientiests indicated that pre-treatment of high fiber feed were able to improve nutrient content and digestibility of feed (Bisaria et al, 1997;Tang et al, 2008;Wanapat et al, 2009 ;Rahman et al, 2011 ;Shrivastava et al, 2011 ;Wajizah et al, 2015 ;Samadi et al, 2015).…”

Produk Metabolisme Rumen pada Sapi Perah Laktasi

“…Oleh karena itu, perlu dilakukan upaya untuk mengatur pola fermentasi rumen, melalui manipulasi ransum sapi. Dilaporkan oleh para peneliti bahwa penambahan yeast dalam ransum sapi perah dapat meningkatkan pH rumen (Harrison et al, 1988;Bach et al, 2007), meningkatkan jumlah dan aktivitas bakteri selulolitik (Wiedmeier et al, 1987;Harrison et al, 1988;Tang et al, 2008) dan pada akhirnya meningkatkan proporsi C 2 /C 3 dari 2,96 menjadi 3,45 (Dawson, 1987). Penambahan biakan yeast dalam ransum juga dapat meningkatkan biomasa mikroba (Dawson, 1987;Harrison et al, 1988), sehingga dapat meningkatkan suplai protein mikroba untuk sapi perah, yaitu 1,3 kali seluruh bakteri rumen dan 1,5 kali bakteri selulolitik (Weidmeir et al, 1987).…”