Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios

Elghandour, Mona M.M.Y.; Kholif, Ahmed E.; Molina, Ofelia Márquez; Vãzquez-Armijo, Josã Fernando; Puniya, Anil Kumar; Salem, Abdelfattah Z.M.

doi:10.3906/vet-1410-26

Cited by 43 publications

(32 citation statements)

References 23 publications

(43 reference statements)

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“…Administrations of exogenous fibrolytic enzymes have been shown to increase in vitro GP and the nutritive value of feeds with ruminal inoculum from cattle [32]. However, in the present study, only cellulase had any effect.…”

Section: In Vitro Fecal Fermentation and Digestibilitycontrasting

confidence: 54%

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The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

Salem

Elghandour

Kholif

et al. 2015

Journal of Equine Veterinary Science

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a b s t r a c tSixteen Quarter Horse mares (450 to 500-kg body weight) were used in a complete randomized design to determine the effects of feeding a high fiber diet with or without exogenous fibrolytic enzymes on nutrient digestion, blood chemistry, fecal coliform count, and in vitro fecal fermentation. The treatments comprised feeding the horses (1) a basal diet without enzyme addition (control); (2) control diet plus cellulase at 10 mL/mare/ d (CELL); (3) control diet plus xylanase at 10 mL/mare/d (XYL); or (4) control diet plus a mixture of 5 mL cellulase and 5 mL xylanase/mare/d (CX). The basal concentrate diet consisted of a mixture of 50% commercial concentrate and 50% wheat bran fed at 4 kg/ horse, offered twice daily at 04:00 and 16:00 hours, and oat straw offered ad libitum at 05:00 and 17:00 hours. The enzyme allocation for each day was mixed with 1 kg of concentrate diet at 04:00 hours, and the experiment lasted for 15 days comprising 10 days of adaptation and 5 days for sample collection. The in vitro cecal fermentation with addition of 2 mL/g dry matter (DM) of each enzyme (CELL, XYL, and CX) to a basal diet of oat straw and concentrates mixture (1:1 DM) as a substrate was carried out. The mares fed enzyme-supplemented diets had greater (P < .01) oat straw and total nutrients intakes compared with the control diet. Feeding enzyme-supplemented diets increased total nutrients digestibility (P < .05) and blood total protein (P ¼ .0277) compared with the control. Feeding XYL-supplemented diet increased blood alanine transaminase and aspartate aminotransferase concentrations (P < .05) compared with control treatment. Lower fecal coliform count was obtained (P ¼ .0114) with mares fed CX diet compared with control mares. The XYL and CX treatments had decreased asymptotic gas production (GP) (P ¼ .0173) with lower rate of GP (P ¼ .0412) compared with CELL treatment. CELL and XYL treatments had decreased (P ¼ .0394) lag times compared with control and CX treatments. At 24 hours of incubation, CELL and XYL treatments decreased methane production (P ¼ .0131), whereas CX treatment increased its production at 48 hours (P ¼ .0202) compared with control treatment. No effect was observed (P > .05) with enzymes addition on carbon dioxide production at different hours of incubation compared with control treatment. Higher in vitro DM degradability values (P ¼ .0092) were obtained with the enzyme

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Section: In Vitro Fecal Fermentation and Digestibilitycontrasting

confidence: 54%

“…Xylanase and cellulose and/or xylanase mixture had no effect. This may be related to the nature of the diet [32] or the fecal inocula used. The higher GP with CELL may allow greater voluntary feed intake by decreasing cecum physical fill and increasing the net energy intake of the diets [33].…”

Section: In Vitro Fecal Fermentation and Digestibilitymentioning

confidence: 99%

The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

Salem

Elghandour

Kholif

et al. 2015

Journal of Equine Veterinary Science

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“…Differences in fibre content, composition, and structure are likely to be related to the variability specificity and activity of ENZ on different roughage sources . Elghandour et al (2015) reported that effects of an exogenous enzyme on ruminal fermentation appear to be substrate-dependent.…”

Section: Discussionmentioning

confidence: 99%

“…Supplementing ruminant diets with fibrolytic enzymes has been shown to improve feed utilization and animal performance (Rojo et al 2015;Morsy et al 2016). However, the effectiveness of enzymes depends upon substrate, enzyme specificity, and enzyme dose (Lara-Bueno et al 2013;Elghandour et al 2015), resulting in variable responses.…”

Section: Introductionmentioning

confidence: 99%

Influence of the addition of exogenous xylanase with or without pre-incubation on the in vitro ruminal fermentation of three fibrous feeds

Elghandour¹,

Kholif²,

Ángeles-Hernández³

et al. 2016

CZECH J ANIM SCI

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The effects of the exogenous fibrolytic enzyme (ENZ) commercial preparation Dyadic® xylanase PLUS (Dyadic International, Inc., Jupiter, USA), containing endo-1,4-β-d-xylanase, on ruminal fermentation of maize stover, oat straw, and sugarcane bagasse were examined using the in vitro gas production (GP) technique. The ENZ commercial preparation was added at 0 (control), 60 (low), 120 (medium), and 240 (high) µg/g dry matter of substrate, and at two times of application (direct addition just before fermentation or with a 72-h pre-incubation before fermentation). Ruminal GP volumes were recorded at 2, 4, 6, 8, 10, 12, 14, 24, and 48 h of incubation, and substrate degradability and concentration of fermentation end-products (volatile fatty acids, ammonia, methane) in the cultures were determined at 48 h of incubation. Increased (P < 0.05) GP volume was observed in fibrous feeds treated with ENZ compared with untreated substrates. The pre-incubation vs the direct addition of ENZ resulted in greater GP volume (P < 0.05) with the three tested feeds. Enzyme addition decreased (P < 0.05) rumen pH compared to control when maize stover and sugarcane bagasse were incubated. Ammonia-N and total volatile fatty acids (VFA) were not affected (P > 0.05) by ENZ application in maize stover and oat straw. However, total and individual VFA concentrations, and CH 4 and CO 2 volumes were greater (P < 0.05) when sugarcane bagasse was incubated with 240 µg ENZ/g (P < 0.05). It can be concluded that the application of endo-1,4-β-d-xylanase enhances rumen fermentation of roughages, although the magnitude of the effects depends on the fibrous substrate fermented, the time of application, and the amount of enzyme added.

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“…3.2.1.8) produced by the fermentation of T. longibrachiatum containing 34,000-41,000 U/g of xylanase activity. These activities were provided by the manufacturers and tested in a previous study (Elghandour et al 2015). The enzyme treatments (CEL, XYL and MIX) were applied at three doses, namely 0, 1 and 3 lL/0.5 g DM.…”

Section: Diet Enzyme Product and Dose Levelmentioning

confidence: 99%

In vitro gas production kinetics and degradability of a diet for growing lambs: effect of fibrolytic enzyme products at different dose levels

López-Aguirre

Hernández-Meléndez

Rojo

et al. 2016

Italian Journal of Animal Science

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The objective of this study was to evaluate the effects of three fibrolytic enzyme products (cellulase (CEL), xylanase (XYL) and a 1:1 mixture of CEL and XYL (MIX)) at three dose levels (0, 1 and 3 lL/0.5 g DM) on the in vitro fermentation of a diet for growing lambs. Bottles were incubated for 96 h at 39 C. A mathematical model was used to estimate the parameters describing the gas production (GP) curve (b, c and L). Dry matter degradability (DMD) and fibre (NDFD and ADFD) degradability were determined at the end of the incubation period. Metabolisable energy (ME) and short chain fatty acids (SCFA) were calculated at 24 h of incubation. The asymptotic GP (parameter b) was affected (p < 0.02) by enzyme product and dose level, with a significant linear response (p < 0.05). Dose level affected ME and SCFA with a significant linear (p < 0.05) and quadratic (p < 0.01) response. The interaction between enzyme product and dose level was significant (p < 0.05) for cumulative GP up to 72 and 96 h of incubation, pH, ADFD and DMD. The results suggest that application of exogenous cellulases has the potential to alter asymptotic GP and degradability of ADF and DM of a diet for growing lambs, but most of the results depend on the interaction between enzyme product and dose level. Future studies are required to determine the ideal combination between enzyme product and dose level for optimal degradation of ruminant feeds. ARTICLE HISTORY

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Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios

Cited by 43 publications

References 23 publications

The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

Influence of the addition of exogenous xylanase with or without pre-incubation on the in vitro ruminal fermentation of three fibrous feeds

In vitro gas production kinetics and degradability of a diet for growing lambs: effect of fibrolytic enzyme products at different dose levels

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