Effects of vitamin D on ovary in DHEA-treated PCOS rat model: A light and electron microscopic study

Çelik, Latife Seyran; Kuyucu, Yurdun; Yenilmez, Ebru Dündar; Tuli, Abdullah; Dağlıoğlu, Kenan; Mete, Ufuk

doi:10.1080/01913123.2017.1385668

Cited by 18 publications

(7 citation statements)

References 25 publications

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“…These findings were in agreement with Çelik et al. (2018) who stated that DHEA induced PCOS due to its effects on the hormones responsible for androgen balance, glucose homeostasis, and regulation of follicular growth. Coinciding with our results, it was found that DHEA increases androgen production which in turn may promote insulin resistance through elevated FBG, HbA1c, and fasting plasma insulin levels associated with increased HOMA‐IR index compared to the control group (Song et al., 2018).…”

Section: Discussionsupporting

confidence: 93%

Effect of fish oil and telmisartan on dehydroepiandrosterone‐induced polycystic ovarian syndrome in rats: The role of oxidative stress, transforming growth factor beta‐1, and nuclear factor kappa B

Kabel

Ashour

Omar

et al. 2020

Food Science & Nutrition

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Our aim was to explore the effect of telmisartan and/or fish oil on dehydroepiandrosterone (DHEA)‐induced PCOS in rats. Sixty female rats were divided into six equal groups as follows: Control; DHEA‐induced PCOS; DHEA + Telmisartan; DHEA + Fish oil; DHEA + Carboxymethyl cellulose; and DHEA + Telmisartan +Fish oil group. Plasma sex hormones, anthropometric measurements, and the glycemic indices were measured. Tissue oxidative stress parameters and the proinflammatory cytokines were assessed. The ovaries were subjected to histopathological and electron microscopic examination. Telmisartan and/or fish oil induced significant improvement of insulin resistance with amelioration of oxidative stress and inflammation compared to PCOS group. Also, telmisartan and/or fish oil restored the hormonal levels and the anthropometric measurements to the normal values. This was significant with telmisartan/fish oil combination compared to the use of each of these agents alone. In conclusion, this combination may represent a promising hope for amelioration of PCOS.

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Section: Discussionsupporting

confidence: 93%

Effect of fish oil and telmisartan on dehydroepiandrosterone‐induced polycystic ovarian syndrome in rats: The role of oxidative stress, transforming growth factor beta‐1, and nuclear factor kappa B

Kabel

Ashour

Omar

et al. 2020

Food Science & Nutrition

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“…Although not all preclinical studies confirmed BW gain after PCOS induction, our results showed that the post-pubertal DHEA-induced model mimics the obese PCOS phenotype, as others confirmed [ 41 ]. On the other hand, pre-pubertal DHEA-induced PCOS models frequently showed an unaltered BW compared to non-PCOS groups [ 42 ], which is one more advantage of the applied post-pubertal DHEA protocol in our study. As shown in Figure 2 n, a final BW was the largest in the P group, while all three treatments decreased BW gain toward control levels.…”

Section: Discussionmentioning

confidence: 76%

Antioxidative Effects of Standardized Aronia melanocarpa Extract on Reproductive and Metabolic Disturbances in a Rat Model of Polycystic Ovary Syndrome

et al. 2022

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Polycystic ovary syndrome (PCOS) represents the most common endocrinopathy among childbearing-age women, with oxidative stress (OS) underlying its etiopathogenesis. Metformin (MET) represents a frequently used agent in PCOS. However, weak results encourage alternative treatments. We aimed to investigate isolated and synergistic effects of Standardized Aronia melanocarpa extract (SEA) and MET for alleviating reproductive and metabolic PCOS abnormalities. PCOS induction was followed by 28-day treatment with MET, SAE, or MET + SEA. Bodyweight (BW), cyclicity, histological, and ultrasonographical ovarian analyses were performed. Hormonal, glycemic, and lipid profiles were accessed, as well as systemic and ovarian oxidative status; BW, cyclicity, ovarian histomorphology, ovarian volume, testosterone and progesterone levels, as well as LDL, triglycerides, and total cholesterol levels were aggravated after PCOS-induction and improved after MET, SEA, and MET + SEA treatment. MET + SEA had the greatest impact on glycoregulation. Alterations in OS parameters (TBARS, O2−, H2O2, catalase, superoxide dismutase, and reduced glutathione) could be responsible for observed differences; (4) Conclusions: Our findings confirmed that SAE alone or along with MET was capable of ameliorating reproductive and metabolic disturbances in the PCOS rat model, with the restoration of OS parameters. SAE alone did not alter the protective effects of MET in PCOS.

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“…In addition, follicles at various stages of development including pre antral and antral follicles as well as corpora lutea were observed. In the related studies, Celik et al reported that Vitamin D3 prevented the formation of PCOM induced by DHEA in rats [ 60 ]. Behmanesh et al also observed that morphology of follicles at various stages of development and corpora lutea were normal when PCOS rats were treated with Vitamin D3 [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Assessing the effect of MitoQ10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

Kyei

Sobhani

Nekonam

et al. 2020

Heliyon

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Polycystic ovary syndrome (PCOS) continues to be one of the most complex reproductive and endocrine disorder among women of reproductive age. Recent reports have identified close interaction of Vitamin D deficiency and oxidative stress (OS) in exacerbating the pathophysiology of PCOS. This current study aims at assessing the combine effect of MitoQ 10 and Vitamin D3 on dehydroepiandrosterone (DHEA) induced PCOS. Following successful induction of PCOS using DHEA, mice were organized into five groups ( n = 8) namely: Negative Control (NC), Vitamin D3 Vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and DHEA, ethanol and distilled water, Vitamin D3, MitoQ 10 and Vitamin D3 plus MitoQ 10 were respectively administered for 20 consecutive days. The study also included positive control (PC) group ( n = 8) in which no treatment was applied. Treatment effects were assessed using hormonal assays, biochemical assays, Real-Time PCR, western blotting and histological analysis. Combination of Vitamin D3 and MitoQ 10 significantly reduced levels of estradiol, progesterone, FSH, LH, LH/FSH, SOD and MDA. The expression rate of mRNAs of 3β-HSD, Cyp19a1, Cyp11a1, StAR, Keap1, HO-1 and Nrf2 were also significantly low in V+M group. Moreover, the histomorphological inspection of ovaries from this group revealed many healthy follicles at various stages of development including few atretic follicles, pre-antral and antral follicles and many corpora lutea. The characteristics observed in this group were in many ways similar to that of the PC group. The combination of MitoQ 10 and Vitamin D3 may be potential candidate to ameliorate PCOS.

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Effects of vitamin D on ovary in DHEA-treated PCOS rat model: A light and electron microscopic study

Cited by 18 publications

References 25 publications

Effect of fish oil and telmisartan on dehydroepiandrosterone‐induced polycystic ovarian syndrome in rats: The role of oxidative stress, transforming growth factor beta‐1, and nuclear factor kappa B

Effect of fish oil and telmisartan on dehydroepiandrosterone‐induced polycystic ovarian syndrome in rats: The role of oxidative stress, transforming growth factor beta‐1, and nuclear factor kappa B

Antioxidative Effects of Standardized Aronia melanocarpa Extract on Reproductive and Metabolic Disturbances in a Rat Model of Polycystic Ovary Syndrome

Assessing the effect of MitoQ10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

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