“…Rivier et al failed to observe GH releasing activity of VIP, PHI or glucagon on rat pituitary cells [13]. In cows, earlier studies demonstrated that VIP exerts a direct action on perifused adenohypophysial tissues to induce GH release, and that the effect of VIP is less potent than that of GHRH [14,15] Bovine GH concentrations in media were measured by a validated RIA method previously described by Johke [17]. The GH standard preparation and hormone for iodination were USDA bGH-Bl [19,20].…”