Effects of ultrahigh pressure extraction conditions on yields of berberine and palmatine from cortex Phellodendri amurensis

Abstract

A new method using ultrahigh pressure was used to extract palmatine and berberine from the barks of cortex Phellodendri amurensis. The optimal conditions for ultrahigh pressure extraction (UPE) of palmatine and berberine were quantified by HPLC-DAD with standard samples. Orthogonal design was applied to evaluate the effects of four independent factors (extraction pressure, extraction temperature, liquid/solid ratio and ethanol concentration) on the yield. The optimal conditions of ultrahigh pressure extraction were obtained at an extraction pressure of 400 MPa, extraction temperature of 40 C, extraction time of 4 min, a liquid/solid ratio of 30 : 1 and an ethanol concentration of 50%. The results showed that the UPE method can produce palmatine and berberine with the highest yield. In addition, scanning electron microscope (SEM) images of the plant cells after ultrahigh pressure treatment were obtained to provide visual evidence of the disruption effect. Compared with other extraction techniques, such as reflux, ultrasonic, and soxhlet extraction, UPE shows several advantages, such as higher extraction yield, less extraction time, lower extraction temperature and energy consumption.Moreover, UPE may be also a better way to be applied in industry.

High-performance liquid chromatography-diode array detector-mass selective detector analysis of major natural dyes with the application of H2O2/ultraviolet treatment as a way to simulate burial degradation of textiles

“…As protoberberine alkaloids, both berberine and palmatine have high radical scavenging activity, thus functioning as antioxidants. [31][32][33] Alizarin in the dye solution tended to be more susceptible to degradation by H 2 O 2 /UV during the initial stage of treatment, whereas with longer treatment the amount of degradation was similar to berberine and palmatine. This was true also for purpurin in the dye solution, although the amount of degradation of purpurin was higher than that of alizarin.…”

Berberine: Botanical Occurrence, Traditional Uses, Extraction Methods, and Relevance in Cardiovascular, Metabolic, Hepatic, and Renal Disorders

“…The interest toward this extraction technique is increasing because it presents several advantages toward classical extraction techniques like increased extraction yields, higher quality of extracts, less extraction time, and decreased solvent consumption (Xi, 2015 ). These are achieved at room temperature by applying different pressure levels (from 100 to 600 MPa) between the interior (higher values) and the exterior of cells (lower values) in order to facilitate the transfer of the bioactive compounds through the plant matrices in the extraction solvent (Liu et al, 2006 , 2013 ). In the study regarding berberine content in Cortex phellodendri , Guoping et al ( 2012 ) made a comparison between UPE, MAE, USE, and heat reflux extraction techniques.…”

Real-time monitoring of the column chromatographic process of Phellodendri Chinensis Cortex part I: end-point determination based on near-infrared spectroscopy combined with machine learning

“…This study aims to apply NIR spectroscopy to determine the elution end-point of the column chromatographic process of Phellodendri Chinensis Cortex , which is one of the natural medicines with the functions of anti-bacterial, anti-inflammatory, anti-cancer and lowering blood pressure. 22–24 Alkaloids are known to be the main active pharmaceutical ingredients in Phellodendri Chinensis Cortex , in which berberine hydrochloride and phellodendrine chloride have clear efficacy and are the index ingredients stipulated in the Chinese Pharmacopeia (2020 ed.) for the quality evaluation of Phellodendri Chinensis Cortex .…”