Real-time monitoring of the column chromatographic process of Phellodendri Chinensis Cortex part I: end-point determination based on near-infrared spectroscopy combined with machine learning

Abstract

As a widely used technique in the area of natural medicine, column chromatographic process lacks appropriate process monitoring methods. In this work, near infrared spectroscopy was applied to develop a...

Real-time monitoring of the column chromatographic process of Phellodendri Chinensis Cortex part II: multivariate statistical process control based on near-infrared spectroscopy

“…This finding was in good accordance with a previously conducted study, which found that alkaloids were eluted fastest in the period from the 60th to 70th min. 10 It can be seen from Fig. 10 that the trajectories of the PC1 scores and DModX of the 16th batch obviously exceeded the control ranges.…”

“…Before the formal elution test started, the macroporous resin was pretreated and the concentrated solution was obtained according to the methods introduced in part I of this work. 10 The added volume of concentrated solution, the elution flow rate, and the ratio of ethanol in the eluent were selected as the factors of interest, which were varied between the settings at three different levels based on the results of the preliminary experiments. 10 A total of 17 experimental runs were obtained after the design (Table 1).…”

“…10 The added volume of concentrated solution, the elution flow rate, and the ratio of ethanol in the eluent were selected as the factors of interest, which were varied between the settings at three different levels based on the results of the preliminary experiments. 10 A total of 17 experimental runs were obtained after the design (Table 1). It should be noted that six batches (1st-6th) were processed under normal operating conditions (NOC), which were used to establish the MSPC model and to evaluate the consistency of the elution process.…”

“…In part I of this work, promising calibration models for berberine hydrochloride, phellodendrine chloride and the total alkaloids were developed for endpoint determination. 10 However, the column chromatography process of natural medicines is complex. The quality fluctuation of raw materials and the abnormal operating parameters usually lead to an abnormal operating status, potentially causing product-quality problems.…”

Optimized identification of cheese products based on Raman spectroscopy and an extreme learning machine

“…This method has the advantages of fast learning speed and good generalization performance. [18][19][20] Taking the Raman spectra of the samples of various brands collected with the integration time of 80 s as an example, the original data were first introduced into an extreme learning machine algorithm, with 72% of the samples as test sets (54 samples) and 28% as training sets (21 samples). The sample selection was random and ran 200 times.…”