Effects of the ultra-high pressure on structure and α-glucosidase inhibition of polysaccharide from Astragalus

Zhu, Zhiwei; Luo, You; Dong, Guo-Ling; Ren, Yuanyuan; Chen, Lijing; Guo, Mingzhe; Wang, Xiaoting; Yang, Xueying; Zhang, Yongmin

doi:10.1016/j.ijbiomac.2016.03.024

Cited by 31 publications

(14 citation statements)

References 20 publications

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“…A growing number of researches have shown that edible fungus polysaccharides are often used as nutrients to regulate immune activity and have massive biological activities, such as antitumor, antioxidant, anti‐inflammatory, hypoglycemic, and hepatoprotective effects [1–4] . And researchers also found that the biological activities of polysaccharides are closely linked to its chemical structures [5–7] …”

Section: Introductionmentioning

confidence: 99%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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In the present study, a novel cold water‐soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853. Its backbone was composed of α‐D‐Glu, α‐D‐Xyl, α‐D‐Gal and α‐L‐Fuc. The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose‐dependent manner. By analyzing Annexin V‐FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria‐mediated apoptotic pathway. Our results could provide valuable information for the potential application of LGP as an anti‐hepatoma agent.

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Section: Introductionmentioning

confidence: 99%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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“…The viscosity of polysaccharides can inhibit glucose diffusion [12,13] maintaining low levels of glucose concentration in the small intestine [12,14]. They are inhibitors of α-D-glucosidases in the bowel, resulting in reduction of carbohydrates' decomposition and absorption [15][16][17]. Plant-derived polysaccharides have shown their anti-inflammatory activities through the modulation of inflammatory mediators and several immune cells [18].…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Chakou

Boual

Hadj

et al. 2021

Plants

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The anti-inflammatory and antihyperglycemic effects of polysaccharides extracted from Alhagi maurorum Medik. seeds, spontaneous shrub collected in Southern of Algerian Sahara were investigated. Their water extraction followed by alcoholic precipitation was conducted to obtain two water-soluble polysaccharides extracts (WSPAM1 and WSPAM2). They were characterized using Fourier transform infrared, 1H/13C Nuclear Magnetic Resonance, Gas Chromatography-Mass Spectrometry and Size Exclusion Chromatography coupled with Multi-Angle Light Scattering. The capacity of those fractions to inhibit α-amylase activity and thermally induced Bovine Serum Albumin denaturation were also investigated. WSPAM1 and WSPAM2 were galactomannans with a mannose/galactose ratio of 2.2 and 2.4, respectively. The SEC-MALLS analysis revealed that WSPAM1 had a molecular weight of 1.4 × 106 Da. The investigations highlighted antinflammatory and antihyperglycemic effects in a dose-dependant manner of WSPAM1 and WSPAM2.

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“…The resulting data of Ra, Rq, Rz from PMP were 0.533 nm, 0.633 nm, 2.94 nm, respectively. The previous study reported the Van der Waals force among molecule chains might interact with negative charges of the mica surface (Zhu et al, ). By contrast, the size of polysaccharides from fermented SCR was much smaller than that of polysaccharides from soybean (Salarb et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…The conformational structure of PMP was determined by Congo red test reported by Zhu et al ().And 1.5 ml PMP aqueous solution (0.5 mg/ml) was added to 0–0.5 M NaOH, which contained 1.5 ml Congo red solution (50 μmol/L). After 15 min, the maximum absorption wavelength ( λ max ) was analyzed by the UV‐visible spectrophotometer in the range of 400–600 nm at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Polysaccharides production from soybean curd residue via Morchella esculenta

Tang

Wei

et al. 2019

J Food Biochem

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To minimize the serious environmental pollution caused by food byproducts, soybean curd residue was used as the substrate to ferment polysaccharides by Morchella esculenta. Water‐soluble crude polysaccharides (CMP) were purified by different gradient concentrations (30%, 60%, and 90%) of ethanol into three precipitation fractions. The experimental results indicated that the precipitated polysaccharides by the ethanol concentration of 60% (PMP) had the highest purity, containing rhamnose, arabinose, xylose, mannose, glucose, and galactose, with the molar ratio of 0.08:0.25:0.16:0.07:0.28:1.00. The physicochemical properties were revealed by SEM, AFM, FTIR, NMR, and Congo red test. Furthermore, the measurement of antioxidant activities in vitro demonstrated that PMP exhibited higher antioxidant capacities with the dose‐dependent manner than CMP and polysaccharides from unfermented soybean curd (USP). Overall, the results suggested that PMP had attractive functional activity which could be potentially utilized in functional food industries. Practical applications The polysaccharides from fungi sources are increasingly being used in functional food and pharmaceutical industry. In allusion to the problem of polysaccharides fermented by edible fungi, such as low yield, high cost, and hard to controllable. Soybean curd residue (SCR), a food waste, has been successfully used for the production of polysaccharides and graded ethanol was used to enhance polysaccharides production, lower the difficulty and homologous costs of purity. The results indicated that the purified polysaccharides displayed excellent antioxidant activities on scavenging DPPH and hydroxyl radicals. The outcomes of this work can provide technical support for comprehensive utilization of SCR and references for promotion and application of polysaccharides fermented from SCR via Morchella esculenta in food and medicine fields.

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Effects of the ultra-high pressure on structure and α-glucosidase inhibition of polysaccharide from Astragalus

Cited by 31 publications

References 20 publications

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Polysaccharides production from soybean curd residue via Morchella esculenta

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