Effects of the special extract ERr 731® from Rheum rhaponticum on estrogen-regulated targets in the uterotrophy model of ovariectomized rats

Long-term effects of the rhapontic rhubarb extract ERr 731® on estrogen-regulated targets in the uterus and on the bone in ovariectomized rats

“…In these studies, subcutaneously administered ERr 731 ® exhibited no estrogenic potential on recovering ovariectomy-induced loss of the uterine wet weight. Beside this the observed ER␤ selectivity of ERr 731 ® in vitro has been supported by the results of co-treatment with E2 [12]. The observed counteracting properties of the extract on some E2-induced responses in the uterus are in line with the Yin-Yang hypothesis of ER action suggested by Gustafsson et al [13,14].…”

“…In accordance with the three-day s.c. application of ERr 731 ® in an uterotrophic assay [12], none of the dosages chosen in this study design had an impact on the uterus wet weight. This is supported by the lack of an increase in the mRNA expression of the proliferation marker genes Mki67 and Pcna.…”

“…The safety of the rhapontic extract ERr 731 ® in terms of proliferative alterations in the uterus of ovariectomized rats has recently been shown by a three-day uterotrophic assay [12]. In the present study we aimed to investigate the safety of a long-term dietary administration of ERr 731 ® as the extract has been administered as tablets in the clinical trials [9,[20][21][22].…”

“…First-strand cDNA synthesis was performed by mixing 3 g of digested RNA with MMLV reverse transcriptase (Promega Corp., Madison, USA) and Oligo (dT) [12][13][14][15][16][17][18] primers.…”

“…Moreover, the estrogenic potential of ERr 731 ® has been tested in an uterotrophic assay in the ovariectomized (ovx) rat [12]. In these studies, subcutaneously administered ERr 731 ® exhibited no estrogenic potential on recovering ovariectomy-induced loss of the uterine wet weight.…”