Effects of the serjania erecta and zeyheria montana ethanol extracts in experimental pulpitis in rats: A histological study

Abstract

Objectives: The aim of this study was to evaluate, by the semi-quantitative histological analysis, the anti-inflammatory activity of the ethanolic extracts of Serjania erecta e Zeyheria Montana, in experimental pulpits in rats. Study Design: In order to induce pulp inflammation, cavities were performed on the occlusal surface of the mandibular first molars of 45 male rats, without pulp exposure. The animals were distributed into 4 groups: GI, teeth without cavities; GII, single dose of saline solution via intraperitoneal (IP); GIII, single dose (IP) of 300mg/Kg of ethanolic extract of Zeyheria montana; GIV, single dose (IP) of 300mg/Kg of ethanolic extract of Serjania erecta. After 6, 12 and 24 hours, 5 animals of each group were killed by anesthetic overdose. The histological analyses of the pulp tissue were performed and the data analyzed by Dunn´s multiple test, at significance of 5%. Results: After 12 h, the GIII presented score statistically lower (p<0.05) than positive control group. After 24 h, GIII presented inflammatory index statistically lower than the positive control (p<0.01) and Serjania erecta (p<0.05) groups. Conclusion: The Zeyheria montana extract presented better anti-inflammatory activity than positive control group and Serjania erecta extract, which did not show anti-inflammatory effect in the analyzed periods. Key words:Anti-inflammatory effect, experimental pulpitis, histological analysis, phytotherapy, rats.

Selenium ameliorates inflammation by decreasing autophagic flux and mitogen‐activated protein kinase signalling on experimentally induced rat periapical lesions

“…At the two levels, two serial sections for each sample were stained with haematoxylin and eosin and examined using a light microscopy (CX21; Olympus Corporation) and an image analysis program (Database Manual Cell Sens Life Science Imaging Software System; Olympus Corporation) by a physician that specializes in pathology. Histopathological changes were graded in a blinded manner as described previously with minor modifications (Nossa et al, 2013). Score 1-normal pulp and no inflammatory reaction; Score 2-slight inflammatory cell infiltration, (1/3 ratio of the inflammatory microscope field), slight hyperemia and oedema; Score 3-moderate hyperemia, inflammation, oedema and small necrotic areas (up to 2/3 ratio of the microscope field); Score 4-marked hyperemia, inflammation, haemorrhage, severe necrosis (more than 2/3 ratio of the microscope field).…”

&lt;i&gt;In Vitro&lt;/i&gt; Propagation and Conservation of &lt;i&gt;Zeyheriamontana&lt;/i&gt; Mart: An Endangered Medicinal Plant

“…Its leaves produce ursolic, oleanolic and betulic acids, pentacyclictriterpenoids, with validated biological properties, such as anticancer activity [1], inhibitory effects on NO production and iNOS induction [2], anti-inflammatory [3], antifungal [4], diuretic [5]. Ethanol extract from Z. montana leaf possesses anti-nociceptive [6] and anti-inflammatory [7] activities and flavanones showed cytotoxicity against human NCI-ADR/RES and K562 cell lines [8].…”