Effects of the herbicides acetochlor and metolachlor on antioxidant enzymes in soil bacteria

Martins, Paulo Sodero; Carvalho, Gabriela Ferreira; Gratão, Priscila Lupino; Dourado, Manuella Nóbrega; Pileggi, Marcos; Araújo, Welington Luiz; Azevedo, Ricardo Antunes

doi:10.1016/j.procbio.2011.02.014

Cited by 75 publications

(49 citation statements)

References 52 publications

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“…Non-denaturing polyacrylamide gel electrophoresis (PAGE) was carried out as described by Martins et al (2011). For denaturing SDS-PAGE, the gels were rinsed in distilled-deionized water and incubated overnight in 0.05% Coomassie blue R-250 in methanol:acetic water 40:7:53 (v/v/v) solution and distained with successive washes in methanol: acetic acid: water 40:7:53 (v/v/v) solution.…”

Section: Polyacrylamide Gel Electrophoresis (Page)mentioning

confidence: 99%

Biochemical and histological characterization of tomato mutants

Monteiro

Rolão

Franco

et al. 2012

An. Acad. Bras. Ciênc.

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Biochemical responses inherent to antioxidant systems as well morphological and anatomical properties of photomorphogenic, hormonal and developmental tomato mutants were investigated. Compared to the non-mutant Micro-Tom (MT), we observed that the malondialdehyde (MDA) content was enhanced in the diageotropica (dgt) and lutescent (l) mutants, whilst the highest levels of hydrogen peroxide (H 2 O 2 ) were observed in high pigment 1 (hp1) and aurea (au) mutants. The analyses of antioxidant enzymes revealed that all mutants exhibited reduced catalase (CAT) activity when compared to MT. Guaiacol peroxidase (GPOX) was enhanced in both sitiens (sit) and notabilis (not) mutants, whereas in not mutant there was an increase in ascorbate peroxidase (APX). Based on PAGE analysis, the activities of glutathione reductase (GR) isoforms III, IV, V and VI were increased in l leaves, while the activity of superoxide dismutase (SOD) isoform III was reduced in leaves of sit, epi, Never ripe (Nr) and green flesh (gf) mutants. Microscopic analyses revealed that hp1 and au showed an increase in leaf intercellular spaces, whereas sit exhibited a decrease. The au and hp1 mutants also exhibited a decreased in the number of leaf trichomes. The characterization of these mutants is essential for their future use in plant development and ecophysiology studies, such as abiotic and biotic stresses on the oxidative metabolism.

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Section: Polyacrylamide Gel Electrophoresis (Page)mentioning

confidence: 99%

Biochemical and histological characterization of tomato mutants

Monteiro

Rolão

Franco

et al. 2012

An. Acad. Bras. Ciênc.

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“…SA did not affect H 2 O 2 production ( Figure 2B) or SOD activity (Figure 3A), under either salt or control growth conditions, but increased CAT activity in the control seedlings was observed ( Figure 3B). On the other hand, the other peroxidase measured, APX ( Figure 3C), and GR ( Figure 3D), an important enzyme of the ascorbate-glutathione cycle, which is involved in responses to oxidative stress in distinct organisms (Monteiro et al, 2011;Martins et al, 2011), did not exhibit any change under the tested conditions. Since SA application during seed priming has been associated with enhanced SOD, CAT, and APX activities in maize under chilling stress , our results with SA priming under salt stress suggest that SA priming, at least in maize, may be a much more complex process.…”

Section: Resultsmentioning

confidence: 92%

Seed priming with hormones does not alleviate induced oxidative stress in maize seedlings subjected to salt stress

Carvalho

Piotto

Schmidt

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Seed priming with hormones has been an efficient method for increasing seed vigor as well as seedling growth under stressful conditions. These responses have in the past been attributed to the activation of antioxidant systems in a range of crops. The results described in this work show that hormonal priming with methyl jasmonate, salicylic acid or CEPA (chloroethylphosphonic acid), an ethylene (ET) releaser, does not induce the antioxidant activity of superoxide dismutase, catalase, ascorbate peroxidase or glutathione reductase in maize seedlings subjected to salt stress. The enhanced biomass of maize seedlings under salt stress that was observed only from ET priming indicates that the stress tolerance in maize from ethylene priming is a fundamental process for stress tolerance acquisition, which is explained, however, by other biochemical mechanisms but not by changes in the antioxidant system.

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“…Bakteri arobik menggunakan enzim katalase ini untuk mendetoksifikasi hidrogen peroksida dan superoksida radikal yang merupakan toksin hasil samping dari metabolisme aerobik (Martins et al, 2011). Bakteri UG3 bersifat oksidase positif yang menunjukkan bahwa bakteri tersebut memiliki sitokrom oksidase sebagai ciri dari bakteri organisme aerob atau anaerob fakultatif (Trevaldi et al, 2010 Polymerase Chain Reaction (PCR).…”

Section: Identifikasi Isolat Bakteri Terpilihunclassified

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

Mulyasari

Widanarni

Suprayudi

et al. 2015

JPBKP

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ABSTRAKPemanfaatan daun singkong sebagai bahan baku pakan ikan belum dilaksanakan secara optimal karena mengandung selulosa yang cukup tinggi. Tuj uan penelitian ini adalah mendapatkan bakteri yang dapat mendegradasi serat daun singkong dan melakukan identifikasi jenis bakteri selulolitik tersebut. Seleksi bakteri selulolitik dilakukan pada 4 jenis isolat bakteri asal saluran pencernaan ikan gurame yaitu UG3, UG6, UG7, dan UG8 dengan mengukur aktivitas enzim selulase secara kuantitatif pada substrat Carboxy Methyl Cellulose (CMC) dan daun singkong menggunakan metode 3,5-dinitrosalicylic acid (DNS). Identifikasi bakteri dilakukan pada 3 isolat yang memiliki aktivitas enzim selulase tertinggi pada substrat daun singkong berdasarkan morfologi (perwarnaan Gram, motilitas dan bentuk bakteri), uji biokimia (oksidase dan katalase) dan analisis gen (16S-rRNA). Hasil penelitian menunjukkan bahwa dari 4 isolat yang diuji cobakan, isolat UG7 memiliki aktivitas enzim selulase (CMCase) tertinggi (0,0043 U/ ml) sedangkan yang terendah adalah UG3 (0,0018 U/ml). Pada uji coba di substrat daun singkong, aktivitas enzim tertinggi terlihat pada isolat UG3 (0,107 U/ml) dan terendah adalah UG6 (0,077 U/ ml). Hasil identifikasi dari 3 jenis isolat bakteri uji yang memiliki kemampuan mendegradasi daun singkong tertinggi (UG3, UG7, dan UG8) menunjukkan bahwa ketiga jenis isolat tersebut merupakan bakteri jenis Gram positif berbentuk batang (basil). Berdasarkan data base dari GenBank, isolat UG3 memiliki kemiripan sebesar 93% dengan Bacillus clausii, UG7 memiliki kemiripan sebesar 96% dengan Bacillus amyloliquefaciens dan UG8 memiliki kemiripan sebesar 88% dengan Bacillus subtilis.

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Effects of the herbicides acetochlor and metolachlor on antioxidant enzymes in soil bacteria

Cited by 75 publications

References 52 publications

Biochemical and histological characterization of tomato mutants

Biochemical and histological characterization of tomato mutants

Seed priming with hormones does not alleviate induced oxidative stress in maize seedlings subjected to salt stress

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

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