Biochemical and histological characterization of tomato mutants

Monteiro, Carolina Cristina; Rolão, Milca B.; Franco, Mônica Regina; Peters, Leila Priscila; Cicarelli, Mariana; Capaldi, Flávia Regina; Carvalho, Rogério Falleiros; Gratão, Priscila Lupino; Rossi, Mônica Lanzoni; Martinelli, Adriana Pinheiro; Peres, Lázaro Eustáquio Pereira; Azevedo, Ricardo Antunes

doi:10.1590/s0001-37652012005000022

Cited by 33 publications

(26 citation statements)

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“…Pesquisadores têm utilizado cultivares miniatura, porém de frutos redondos, denominada Mini-Tom (MT), como modelo genético para estudos básicos, genéticos e fisiológi-cos (WANG et al, 2005;WATANABE et al, 2007;SILVA et al, 2011;MONTEIRO et al, 2012;PÉ-REZ;AHMED, CABEZAS, 2013). No entanto, pouco se sabe a respeito do tipo de herança condicionado pelas plantas anãs ao cruzar com plantas de hábito de crescimento indeterminado.…”

Section: Introductionunclassified

Ocorrência De Nanismo Em Planta De Tomateiro Do Tipo Grape

Maciel

Fernandes

2015

Rev. Caatinga

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RESUMO -O conhecimento genético de manifestações fenotípicas de características de importância agronô-mica em vegetais é uma atividade de grande importância, cujos resultados são amplamente utilizados por fitomelhoristas. O objetivo do presente trabalho foi estudar o tipo de segregação genética que ocorre ao se cruzar um genótipo anão versus genótipo de hábito de crescimento indeterminado. A referida planta foi encontrada vegetando espontaneamente no município de Piracicaba (SP) em local de descarte de frutos por tomaticultores juntamente com as plantas normais. As sementes de ambas as plantas foram colhidas separadamente para início do estudo e codificadas da seguinte forma: planta com fenótipo normal (FN) e planta com fenótipo atípico com características de porte anão (FA). A metodologia constou de cruzamentos biparentais recíprocos e retrocruzamentos da geração F 1 com o genitor FA e obtenção de geração F 2 . Os dados obtidos oriundos da contagem de plantas das populações obtidas (F 1 , F 2 e F 1 RC 1 ) foram submetidos ao teste de χ 2 de acordo com a frequência esperada e observada do fenótipo normal (FN) e planta com fenótipo atípico com características de porte anão (FA), tendo como hipótese uma segregação mendeliana 3:1, obtida para herança monogênica. Pelos resultados obtidos foi possível concluir que o fenótipo observado na planta no presente estudo é de origem genética recessiva, podendo ser transmito para outras plantas via cruzamento. Palavras-chave:Solanum lycopersicum L. Gene d (Dwarf). Herança. Alelo. Mutação. DWARFISM OCCURRENCE IN TOMATO PLANT TYPE GRAPEABSTRACT -The phenotypic manifestations of genetic knowledge of important agronomic traits in plants is an activity of great importance, whose results are widely used in plant breeding. The objective was to study the type of genetic segregation that occurs when crossing a dwarf genotype versus indeterminate growth habit genotype. Their plant was found growing spontaneously in Piracicaba (SP), on-site disposal of fruits per tomatoes producers along with normal plants. Seeds of both plants were harvested separately for baseline and coded as follows: a plant with a normal phenotype (FN) and plant with atypical phenotype with characteristics of dwarfism (FA). The methodology consisted of biparental and reciprocal backcrosses of the F 1 to the parent FA and obtained the F 2 generation. The data derived from the plant count populations obtained (F 1 , F 2 and backcrosses) were tested using the χ 2 according to the expected frequency and observed the normal phenotype (FN) and plant with atypical phenotype with characteristics of dwarfism (FA), under the hypothesis Mendelian segregation 3:1, obtained by monogenic inheritance. From the results, it can be concluded that the phenotype observed in the plant in this study is recessive genetic origin can be inserted into other plants by crossing.

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Section: Introductionunclassified

Ocorrência De Nanismo Em Planta De Tomateiro Do Tipo Grape

Maciel

Fernandes

2015

Rev. Caatinga

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“…Diferentemente do mutante aurea, que deteve maiores atividades destas enzimas antioxidativas, quando comparado ao mutante hp1, mesmo com conteúdo de peróxido de hidrogênio inferior ao mutante hp1 (MONTEIRO et al, 2012). Nesse sentido, podese inferir que, embora não tenham sido observadas, neste trabalho, alterações significativas no conteúdo de compostos fenólicos e capacidade antioxidante em frutos do mutante aurea, quando comparado aos da cultivar selvagem 'MT' (Figura 1), não se descarta a influência desta mutação sobre outros componentes do aparato antioxidativo não contemplados pelas análises efetuadas neste trabalho.…”

Section: Resultsunclassified

Compostos fenólicos e capacidade antioxidante em frutos de tomateiros mutantes fotomorfogenéticos

Dias

Melo²,

Alves³

et al. 2015

Cienc. Rural

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“…For example, the hypocotyls of the au tomato mutant, which is phytochrome-deficient (Muramoto et al, 2005), have reduced levels of anthocyanins, which are important antioxidant molecules (Carvalho et al, 2010). Moreover, the leaves of au exhibited reduced lipid peroxidation and enhanced H 2 O 2 (Monteiro et al, 2012), generating strong perspectives on the biochemical and molecular manipulation of light signaling. In addition, the far red light insensitive (fri) mutant of tomato (van Tuinen et al, 1995), which is phyA-deficient, produces fruits with important qualities for the paste industry, such as an increased dry weight/fresh weight ratio, total soluble solids, and paste viscosity, and fewer seeds per fruit compared with the wild type (Alba et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Nutrition in tomato (Solanum lycopersicum L) as affected by light: revealing a new role of phytochrome A

Carvalho¹,

Moda²,

Silva³

et al. 2016

Aust J Crop Sci

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The far red light insensitive (fri) mutant of tomato, which is phytochrome A (phyA) deficient, displays some characteristics that have recently indicated important functions of this photoreceptor in water relations. With respect to the relationship between nutrition and water relations, we investigated the growth and nutritional status of fri supplied with Hoagland's complete solution and solutions with the individual omission of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulfur (S). For this purpose, 20-day-old tomato plants of the WT (cv. Moneymaker) and fri mutant were transplanted into pots (one plant per pot) that contained Hoagland and Arnon (1950) solution diluted to 50 per cent in the first week and to 100 per cent from the second week of cultivation until the end of the experiment (50 DAT O) from a balanced nutrient solution. The experiment was arranged in a completely randomized factorial design with two genotypes and seven types of nutrient solutions with three replications. Upon harvest, the following measurements were performed: the height of the plants, measured from the base of the stem of each plant to the insertion of the first fully expanded leaf; the stem diameter; the total number of leaves per plant; an indirect chlorophyll measurement, which we called the green color index, and the leaf area. Statistical analysis was performed using analysis of variance (ANOVA) followed by Tukey's test. First, based on growth analyses, fri showed an enhanced dry weight of the shoot, root and whole plant in complete solution compared with the wild type (WT). In addition, the phyA mutant had a multifaceted response compared with that of the WT when the nutrients were omitted. For the fri mutant, the height and green color index were reduced without N and K; the leaf area without P, K and S; the dry weight of the root and shoot without N, P, K and S, and the root, shoot and total plant dry weight without P, K and S. On the other hand, the green color index of the fri mutant was enhanced without Ca and Mg. Together, these results show that in addition to revealing an altered response to nutrition in the fri mutant, phyA can play a role in light signaling in the nutrition and nutritional stress of tomato.

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Biochemical and histological characterization of tomato mutants

Cited by 33 publications

References 50 publications

Ocorrência De Nanismo Em Planta De Tomateiro Do Tipo Grape

Ocorrência De Nanismo Em Planta De Tomateiro Do Tipo Grape

Compostos fenólicos e capacidade antioxidante em frutos de tomateiros mutantes fotomorfogenéticos

Nutrition in tomato (Solanum lycopersicum L) as affected by light: revealing a new role of phytochrome A

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