Effects of the Ga‐As laser irradiation on myonecrosis caused by <i>Bothrops Moojeni</i> snake venom

Dourado, Doroty Mesquita; Favero, Silvio; Baranauskas, Vı́tor; Cruz‐Höfling, Maria Alice da

doi:10.1002/lsm.10237

Cited by 51 publications

(40 citation statements)

References 18 publications

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“…The optical laser power was determined by a Newport 1835-C Multi Function Optical Power Meter® (Newport Corp., USA). Laser dose was low enough to avoid any thermal effect and chosen based on studies that had shown a beneficial effect of low-level laser therapy on the inflammatory process and myonecrotic effect (24,(27)(28)(29).…”

Section: Laser Devicementioning

confidence: 99%

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

Barbosa¹,

Villaverde²,

Guimarães-Sousa³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema formation, neutrophil accumulation and myonecrosis induced by both myotoxins 24 hours after the injection. LLLT reduced the myonecrosis caused by BthTX-I and BthTX-II, respectively, by 60 and 43%; the edema formation, by 41 and 60.7%; and the leukocyte influx, by 57.5 and 51.6%. In conclusion, LLLT significantly reduced the effect of these snake toxins on the inflammatory response and myonecrosis. These results suggest that LLLT should be considered a potential therapeutic approach for treatment of local effects of Bothrops species venom.

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Section: Laser Devicementioning

confidence: 99%

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

Barbosa¹,

Villaverde²,

Guimarães-Sousa³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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“…The control animals were inoculated with a sterile 0.9% saline solution. and Dourado et al (10). The average wattage of the equipment was measured before the experiment with the aid of a power-measuring device (2-watt broadband power/energy meter, 13 PEM 001/J®, Melles Griot, The Netherlands).…”

Section: Venom Inoculationmentioning

confidence: 99%

“…This leads to a catalytic process involving diverse substances, giving rise to a series of inflammatory reactions (12). Due to their catalytic function, these substances can exhibit toxic or pharmacological effects such as myonecrosis, neurotoxicity and cardiotoxicity as well as hemolytic, hemorrhagic, hypotensive and anticoagulant activities and inhibition of platelet aggregation (10).…”

Section: Creatine Kinase (Ck) Activity In Plasmamentioning

confidence: 99%

“…A large number of researchers have been looking for new therapeutic methods to repair or at least minimize the damage caused by Bothrops snake venom to the muscles (3,5,10). Low-level laser therapy can be considered an alternative if we consider its sequelae on different types of biological tissues -including its trophicregenerative, anti-inflammatory and analgesic effectswhich have been demonstrated in both in vitro and in vivo studies (2, 4, 6-8, 11, 18, 19, 21, 24-26).…”

Section: Introductionmentioning

confidence: 99%

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The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom

Dourado¹,

Matias²,

Almeida³

et al. 2008

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:The present study aimed to assess the effects of low-level laser (660 nm) on myonecrosis caused by the insertion of Bothrops neuwiedi venom in the gastrocnemius muscle of rats. Male Wistar rats were divided into three groups (n = 24 each): Group S (0.9% saline solution); Group V (venom) and Group VLLL (venom plus low-level laser). These categories were subdivided into four additional groups (n = 6) based on the euthanasia timing (3 hours, 24 hours, 3 days and 7 days). The groups V and VLLL were inoculated with 100 µL of concentrated venom (40 µg/mL) in the gastrocnemius muscle. The muscle was irradiated using a gallium-aluminumarsenide laser (GaAlAs) at 35 mW power and 4 J/cm² energy density for 3 hours, 24 hours, 3 days or 7 days after venom inoculation. To evaluate the myotoxic activity of the venom, CK activity was measured and the muscle was histologically analyzed. The low-level laser reduced venom-induced CK activity in the groups euthanized at 3 hours, 24 hours and 3 days (p < 0.0001). Histological analysis revealed that low-level laser reduced neutrophilic inflammation as well as myofibrillar edema, hemorrhage and myonecrosis following B. neuwiedi envenomation. These results suggest that low-level laser can be useful as an adjunct therapy following B. neuwiedi envenomation.

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“…En otros estudios morfológicos, se ha observado un efecto bioestimulatorio en el tejido fibroconectivo (Vinck et al, 2003;Shu et al, 2002y Matamala et al, 2001. En músculos de mamíferos lesionados por diversas causas, se ha reportado un aumento de tejido funcional en procesos regenerativos (Weiss & Oron, 1992;Houghton & Brown, 1999;Amaral et al, 2001;Oron et al, 2001;Dourado et al, 2003). Además, se ha observado un efecto similar en músculo esquelético de anfibios (Bibikova & Oron, 1993).…”

Section: Introductionunclassified

Efecto Morfológico de la Irradiación Láser AsGa en el Músculo Esquelético de Rata

2007

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RESUMEN:La terapia láser AsGa es ampliamente utilizada en la rehabilitación de lesiones articulares y tendinosas. Por las características físicas del haz de luz utilizado, parte de esta irradiación es absorbida por tejido muscular adyacente, por lo que se planteó medir en un modelo experimental en rata, posibles efectos morfológicos de la irradiación láser AsGa en este tejido. Se realizó un estudio morfométrico y estereológico, utilizando ratas Sprague Dawley sanas, las que fueron irradiadas, por 10 días, en la zona del vientre del músculo gastrocnemio, utilizando tres diferentes dosis 0,8 (A1 y A2), 2 (B) y 5 (C) joules/cm 2 . Los miembros contralaterales no fueron irradiados (control).Se determinó el área de la fibra muscular por recuento de puntos (morfometría) en grupo A1. La densidad de volumen de tejido conectivo se midió a través un sistema test M42 (estereología) en los grupos A2, B y C.Los resultados obtenidos mostraron que el área de las fibras musculares fue significativamente mayor (p=0,001) en los músculos irradiados que en sus controles (1513±376 mm 2 -1146±286 mm 2 ). El análisis estereológico de la densidad de volumen de tejido conectivo (Vvtc) indicó que no existieron diferencias significativas (p=0,07) entre músculos irradiados y controles, en ninguna de las tres dosis utilizadas. Los resultados de este estudio indican que existe, a nivel morfológico, un efecto aparentemente hipertrófico del láser AsGa en el músculo esquelético de rata.

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Effects of the Ga‐As laser irradiation on myonecrosis caused by Bothrops Moojeni snake venom

Abstract: The laser treatment significantly inhibited the ability of B. moojeni venom to rapidly disrupt the integrity of the plasma membrane.

Cited by 51 publications

References 18 publications

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom

Efecto Morfológico de la Irradiación Láser AsGa en el Músculo Esquelético de Rata

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