Efecto Morfológico de la Irradiación Láser AsGa en el Músculo Esquelético de Rata

The purpose of the present work was to study the effect of low-level laser therapy (LLLT): helium-neon (He-Ne) and gallium arsenide (Ga-As) laser on the histomorphology of muscle and mitochondria in experimental myopathy in rats. Thirty Suquía strain female rats were distributed in groups: (A) control (intact), (B) injured, (C) injured and treated with He-Ne laser, (D) injured and treated with Ga-As laser, (E) irradiated with He-Ne laser on the non-injured muscle, and (F) irradiated with Ga-As laser on the non-injured muscle. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left gastrocnemius muscle at the same point on five consecutive days, in groups B, C, and D. LLLT was applied with 9.5 J cm daily for seven consecutive days in groups C, D, E, and F. The muscles were examined with optic and electronic microscopy. The inflammation was classified as absent, mild, and intense and the degree of mitochondrial alteration was graded I, II, III, and IV. Categorical data were statistically analyzed by Chi-square and the Fisher-Irwin Bilateral test, setting significant difference at p < 0.05. The damage found in muscle and mitochondria histomorphology in animals with induced myopathy (B) was intense or severe inflammation with grade III or IV of mitochondrial alteration. They underwent significant regression (p < 0.001) compared with the groups treated with He-Ne (C) and Ga-As (D) laser, in which mild or moderate inflammation was seen and mitochondrial alteration grades I and II, recovering normal myofibrillar architecture. No differences were found between the effects caused by the two lasers, or between groups A, E, and F. Group A was found to be different from B, C, and D (p < 0.001). LLLT in experimental myopathy caused significant muscular and mitochondrial morphologic recovery.

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Histomorphologic and ultrastructural recovery of myopathy in rats treated with low-level laser therapy

Servetto

Cremonezzi

Simes

et al. 2017

Lasers Med Sci

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Efectos de la sobredosificación con láser de arseniuro de galio sobre el cuádriceps de ratas

Pallares

et al. 2012

Rev MVZ Córdoba

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RESUMENObjetivo. Obtener información de los efectos de la irradiación con laser con el objeto de determinar dosis en la que se observen efectos deletéreos sobre el tejido muscular. Materiales y métodos. Se estudiaron 20 ratas macho Sprague Dawley y fueron divididas aleatoriamente en cuatro grupos de 5 individuos. En todos los grupos se realizó la irradiación con laser, empleando un equipo de diodo de Arseniuro de Galio. Se aplicó en forma puntual 1 vez al día durante 10 días consecutivos. El tiempo de exposición y las dosis diarias siguieron el siguiente arreglo: grupo A, 15 minutos y 24.3 julios, grupo B, 30 minutos y 48.6 Julios, grupo C, 45 minutos y 72.9 Julios, grupo D, 60 minutos y 97.2 Julios. Se tomaron muestras del cuadriceps para microscopía óptica y microscopía electrónica. Resultados. La microscopia óptica no mostró alteraciones en ninguno de los grupos estudiados. Al microscopio electrónico se observaron alteraciones ultraestructurales solamente en el grupo D. Conclusiones. El rango entre la dosis terapeútica y la que produce alteraciones ultraestructurales en el tejido muscular es tan amplio que hace del laser un eficiente elemento seguro para la terapia física.Palabras clave: Laser, músculo, ratas, sobredosis (Fuente: CAB). ABSTRACTObjective. To obtain information on the effects of laser irradiation in order to determine maximum free of deleterious effects dose on muscle tissue. Materials and methods. Twenty male Sprague Dawley rats were studied randomly divided into four groups A, B, C and D. All groups were irradiated with a Laser, using a Gallium Arsenide diode machine. Irradiation was applied in the same point once a day for 10 consecutive days. Groups: A: 15 minutes a day, with a total dose of 24.3 Joules, B: 30 minutes, 48.6 Joules, 45 minutes, 72.9 Joules C: D: 60 minutes, 97.2 joules. Samples from quadriceps were taken for optical and electron microscopy. Results. No alterations were exposed under optical microscopy in any of the groups studied. The electron micrographies showed ultrastructural alterations only in D Group. Conclusions. The difference between the therapeutic dose and the doses that produce ultrastructural alterations in muscle tissue is so great that it make of laser an efficient and safe element to be used in physical therapy.

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Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles

et al. 2011

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SUMMARY:The administration of microencapsulated drug in a matrix acid poly(lactic-co-glycolic acid) (PLGA) by intramuscular (IM) in humans has been approved by the FDA for various applications though it is not clear what effect they have on the morphological parameters of muscle tissue. The aim of this study was to analyze the morphological changes in the skeletal muscle tissue with their use. We used 12 adult female Sprague Dawley rats (Rattus novergicus) that were injected into their right gastrocnemius muscle belly with: sterile vehicle solution (G1, n = 4), 0.5 mg PLGA microparticle (G2, n = 4) and 0.75 mg PLGA microparticle (G3, n = 4), both dissolved in a sterile vehicle solution. At 14 days post injection the number and diameter of muscle fibers, the level of inflammation and histology appearance in terms of organization of muscle fibers, cellular distribution, tissue morphology and the presence of polymer waste were determined and the results between the groups compared. The administration of the compound in a single dose did not alter the morphometric parameters (number and diameter of muscle fibers) despite generating a mild inflammation in the tissue associated with the presence of polymeric residues, suggesting that the PLGA microparticles were well tolerated by the muscle tissue at concentrations tested (0.5 and 0.75 mg).

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Efecto Morfológico de la Irradiación Láser AsGa en el Músculo Esquelético de Rata

Cited by 3 publications

References 15 publications

Histomorphologic and ultrastructural recovery of myopathy in rats treated with low-level laser therapy

Histomorphologic and ultrastructural recovery of myopathy in rats treated with low-level laser therapy

Efectos de la sobredosificación con láser de arseniuro de galio sobre el cuádriceps de ratas

Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles

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