Effects of Temperature, Soil Moisture, and Wheat Bran on Growth of<i>Trichoderma harzianum</i>from Alginate Pellets

Knudsen, G. R.

doi:10.1094/phyto-80-724

Cited by 72 publications

(55 citation statements)

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“…Espécies de Trichoderma aplicadas de forma isolada, associadas com Gliocladium virens Miller, Giddens & Foster ou com espécies bacterianas têm sido extensivamente estudadas no controle de R. solani (Hadar et al, 1979;Elad et al, 1980;Harman et al, 1980;Chet & Baker, 1981;Nelson et al, 1983;Beagle-Ristaino & Papavizas, 1985;Kwok et al, 1987;Lewis & Papavizas, 1987;Knudsen & Bin, 1990;Lewis et al, 1998). A ação antagonista de Trichoderma spp.…”

Section: Encapsulation Of Trichoderma Inhamatum For the Biological Counclassified

“…proliferam abundantemente, especialmente quando aplicado como micélio jovem veiculado em farelo de trigo (Lewis & Papavizas, 1985). Com o farelo de trigo apesar de não ter influenciado no crescimento radial de isolados de Trichoderma spp., obteve-se maior densidade de hifas (Knudsen & Bin, 1990). Esta fonte alimentar foi considerada como o melhor meio para o crescimento e esporulação de T. harzianum (Hadar et al, 1979).…”

Section: Fitopatol Bras 28(1) Jan -Fev 2003 Rg Mafia Et Alunclassified

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Encapsulamento de Trichoderma inhamatum para o controle biológico de Rhizoctonia solani na propagação clonal de Eucalyptus

et al. 2003

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Testou-se um novo sistema para o encapsulamento de Trichoderma inhamatum em grânulos de alginato de sódio, visando o controle biológico de Rhizoctonia solani, agente etiológico da mela de estacas/miniestacas de Eucalyptus spp. para enraizamento. No novo sistema idealizado, foi utilizado um aparato simples capaz de substituir eficientemente o equipamento (Bomba Peristáltica) anteriormente utilizado, sendo possível aumentar a produção de 594 grânulos/min para aproximadamente 6.734 grânulos/min. Com este novo sistema, um isolado de T. inhamatum (UFV – 03) foi encapsulado em grânulos contendo as fontes alimentares: farelo de trigo, palha de arroz, farelo de aveia, folhas de eucalipto ou farelo de milho na concentração de 50 g/l. Na segunda etapa, a melhor fonte alimentar foi testada nas concentrações de 0 a 60 g/l. Os grânulos foram veiculados em substrato de enraizamento de eucalipto na concentração de 2% (p/p) inoculado com micélio triturado de R. solani (2 mg/g de substrato) e a atividade saprofítica do patógeno foi quantificada por meio do método de iscas. Posteriormente, os grânulos produzidos com a fonte alimentar e concentração que promoveram maior inibição do desenvolvimento de R. solani foram utilizados para determinar o tempo mínimo de pré-incubação e competição para supressão do patógeno, com a mesma metodologia. Observou-se aumento da supressão da atividade saprofítica de R. solani ao acréscimo de uma fonte alimentar. Daquelas testadas, farelo de trigo foi a melhor. Além disso, houve interação significativa e positiva ao aumento de sua concentração na formulação.

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Section: Encapsulation Of Trichoderma Inhamatum For the Biological Counclassified

Section: Fitopatol Bras 28(1) Jan -Fev 2003 Rg Mafia Et Alunclassified

Encapsulamento de Trichoderma inhamatum para o controle biológico de Rhizoctonia solani na propagação clonal de Eucalyptus

et al. 2003

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“…For example, Knudsen and coworkers (11,18) quantified the influences of temperature, soil matric potential, nutrient source, and antagonistic bacteria on the hyphal growth and biocontrol efficacy of pelletized T. harzianum in soils. However, it was not possible to differentiate the hyphal growth of this fungal agent from that of indigenous Trichoderma strains (3,18).…”

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confidence: 99%

Cotransformation of Trichoderma harzianum with β-Glucuronidase and Green Fluorescent Protein Genes Provides a Useful Tool for Monitoring Fungal Growth and Activity in Natural Soils

Bae

Knudsen

2000

Appl Environ Microbiol

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Trichoderma harzianum was cotransformed with genes encoding green fluorescent protein (GFP), ␤-glucuronidase (GUS), and hygromycin B (hygB) resistance, using polyethylene glycol-mediated transformation. One cotransformant (ThzID1-M3) was mitotically stable for 6 months despite successive subculturing without selection pressure. ThzID1-M3 morphology was similar to that of the wild type; however, the mycelial growth rate on agar was reduced. ThzID1-M3 was formed into calcium alginate pellets and placed onto buried glass slides in a nonsterile soil, and its ability to grow, sporulate, and colonize sclerotia of Sclerotinia sclerotiorum was compared with that of the wild-type strain. Wild-type and transformant strains both colonized sclerotia at levels above those of indigenous Trichoderma spp. in untreated controls. There were no significant differences in colonization levels between wild-type and cotransformant strains; however, the presence of the GFP and GUS marker genes permitted differentiation of introduced Trichoderma from indigenous strains. GFP activity was a useful tool for nondestructive monitoring of the hyphal growth of the transformant in a natural soil. The green color of cotransformant hyphae was clearly visible with a UV epifluorescence microscope, while indigenous fungi in the same samples were barely visible. Green-fluorescing conidiophores and conidia were observed within the first 3 days of incubation in soil, and this was followed by the formation of terminal and intercalary chlamydospores and subsequent disintegration of older hyphal segments. Addition of 5-bromo-4-chloro-3-indolyl-␤-D-glucuronic acid (X-Gluc) substrate to recovered glass slides confirmed the activity of GUS as well as GFP in soil. Our results suggest that cotransformation with GFP and GUS can provide a valuable tool for the detection and monitoring of specific strains of T. harzianum released into the soil.

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“…The effect of high temperatures on Trichoderma storage was also tested by Küçük and Kivanç (2005), but they were unable to recover colonies after six weeks storage at 30ºC. On the opposite, experiments run with alginate encapsulated mycelia showed that radial T. harzianum (ThzID1) development was better when inoculated in slit loamy soil and incubated during 14 days at 24ºC than at 15ºC (Knudsen and Bin, 1990). Their differences with our results could be explained on the different Trichoderma strains and type of soils used for experiments, because formulations did not differ significantly.…”

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confidence: 99%

Biocontrol capacity of wild and mutant Trichoderma harzianum (Rifai) strains on Rhizoctonia solani 618: effect of temperature and soil type during storage

Montealegre¹,

Valderrama²,

Herrera³

et al. 2009

Electro Journal of Biotech

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Wild (Th11, Th12 and Th650) and mutant (Th11A80.1, Th12A40.1, Th12C40.1 and Th650-NG7) Trichoderma harzianum strains were stored for 180 days at 5ºC or at 22ºC, in two types of soils. Strains recovered at 90, 120 and 180 days from the two types of soils, retained their full capacity to biocontrol Rhizoctonia solani 618, that produces crown and root rot of tomatoes. Recovery, estimated as colony forming units (cfu) of the wild and mutant strains, showed that all increased their cfu after storage independently of the type of soil and temperature, although kinetic behavior differed among strains. Ratios of recovery after storage in type B soil/ type A soil or at 22ºC/5ºC, higher or lower than one respectively, allowed to establish that Th11 and Th12 were the most appropriate strains for the biocontrol of R. solani in conditions where growth of the phytopathogen is optimal.

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Effects of Temperature, Soil Moisture, and Wheat Bran on Growth ofTrichoderma harzianumfrom Alginate Pellets

Abstract: Knudsen, G. R., and Bin, Li. 1990. Effects of temperature, soil moisture, and wheat bran on growth of Trichoderma harzianum from alginate pellets. Phytopathology 80:724-727.

Cited by 72 publications

References 1 publication

Encapsulamento de Trichoderma inhamatum para o controle biológico de Rhizoctonia solani na propagação clonal de Eucalyptus

Encapsulamento de Trichoderma inhamatum para o controle biológico de Rhizoctonia solani na propagação clonal de Eucalyptus

Cotransformation of Trichoderma harzianum with β-Glucuronidase and Green Fluorescent Protein Genes Provides a Useful Tool for Monitoring Fungal Growth and Activity in Natural Soils

Biocontrol capacity of wild and mutant Trichoderma harzianum (Rifai) strains on Rhizoctonia solani 618: effect of temperature and soil type during storage

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