Effects of temperature, incubation period and substrate on production of fusaproliferin by Fusarium subglutinans ITEM 2404

Abstract

The kinetics of the production of fusaproliferin by Fusarium subglutinans ITEM 2404 in maize and rice cultures was investigated at various incubation temperatures. The growth rate of F. subglutinans was highest at 20 degrees C and 25 degrees C in maize cultures and at 15 degrees C in rice cultures. Although the growth rate was higher in rice than in maize, the maximal production of fusaproliferin was obtained in maize cultures, with a maximum yield (4309 microg g(-1)) at 20 degrees C for 6 weeks. In rice cultures the optimal incubation regimen was at 15 degrees C for 6 weeks, with a fusaproliferin level of 1557 microg g(-1). The production of fusaproliferin at 25 degrees C and 30 degrees C in both substrates was very low, with maximal yield at 25 degrees C of 979 microg g(-1) after 2 weeks and 143 microg g(-1) after 3 weeks in maize and rice cultures, respectively.

Relationship between European Corn Borer injury, Fusarium proliferatum and F. subglutinans infection and moniliformin contamination in maize

“…Both of the Fusarium species responsible for MON contamination can be found throughout the world, but as reported by Doohan et al (2003), the optimum temperature for the growth of F. subglutinans in laboratory conditions is lower (Temperature = 15-25 • C, Castellá et al, 1999) than that of F. proliferatum (Temperature = 30 • C, Marín et al, 1995). As far as the European distribution and prevalence of the two different Fusarium species that cause MON production is concerned, F. proliferatum is more common in southern European areas, while it is substituted by F. subglutinans in central areas .…”

Influence of H2O2-Induced Oxidative Stress on In Vitro Growth and Moniliformin and Fumonisins Accumulation by Fusarium proliferatum and Fusarium subglutinans

“…Among the emerging mycotoxins, the health concerns for MON produced by F. proliferatum and F. subglutinans has been ascribed increasing importance. Due to their different environmental needs [ 12 , 49 , 50 ], F. proliferatum is more common in warm areas of southern Europe, including Italy [ 5 ]. However, the spread of F. subglutinans from the humid and cool areas of north-western and central Italy [ 3 , 51 ] could represent a potential risk for MON contamination in the future, as a consequence of climate change.…”

Optimization of Nutrients and Process Parameters for Improved Production of Bioactive Metabolite Butyrolactone I by Aspergillus terreus strains under Submerged Fermentation

“…Temperature is one of the most important environmental process parameter influencing the growth and production of secondary metabolites by fungi (Castella et al, 1999, Ramos et al, 1998. Both the strains of A. terreus produced maximum Butyrolactone I at incubation temperature of 30 o C (Table 5), hence temperature of 30 o C was found to be the optimum for the production of Butyrolactone I.…”