Optimization of Nutrients and Process Parameters for Improved Production of Bioactive Metabolite Butyrolactone I by Aspergillus terreus strains under Submerged Fermentation

Abstract

Aspergilli, a large and diverse genus of ubiquitous filamentous fungi are the source of diverse secondary metabolites that can be used in the development of medications to treat diseases. Butyrolactone I is produced as a secondary metabolite by A. terreus. Butyrolactone I is a potent inhibitor of the eukaryotic cyclin-dependent kinases (CDK's), protein kinases which control cell progression in all eukaryotes. Cyclin-dependent kinases are involved in numerous diseases in human beings like, cancer, stroke, diabetes, inflammation and AIDS. Butyrolactone I can become a life saving molecule in the above said diseases. In the present investigation the concentrations of carbon, nitrogen and phosphate sources and different fermentation conditions like temperature, media pH, agitation and incubation period were screened for their effect on the production of Butyrolactone I by two strains of A. terreus. The optimum nutrient concentrations and fermentation conditions for maximum production of Butyrolactone I were identified.