Background and Objective: Kalanchoe tomentosa is identified and their different characteristics regarding the antibacterial and antioxidant properties have a vast effect. Fresh K. tomentosa leaves obtained from Bandung, Indonesia was extracted using n-hexane followed by serial dichloromethane maceration. Materials and Methods: N-hexane and ethyl acetate were used to separate the dichloromethane extract using vacuum liquid chromatography and the isolated compounds were recrystallized with n-hexane. Results: About 37 mg of dichloromethane extract was obtained from the extraction process. Recrystallized compound isolates were identified as stigmast-5-en-3-ol or $-sitosterol. Both dichloromethane extract and $-sitosterol isolated compounds showed strong bacteriostatic activity against S. aureus with MIC = 15.63 and 7.81 µg mLG 1 and K. pneumonia with MIC = 7.81 and 31.25 µg mLG 1 , respectively. However, only dichloromethane extract exhibited a bactericidal effect (7.81 µg mLG 1 ). Conclusion: The pure $-sitosterol compound was isolated from K. tomentosa dichloromethane extract. Both the dichloromethane extract and the isolated $-sitosterol compound had antibacterial effects against S. aureus and K. pneumonia.