Effects of Sodium Azide (NaN3) on Seeds Germination, Plantlets Growth and In vitro Antimalarial Activities of Phyllanthus odontadenius Müll. Arg.

Nakweti, Rufin Kikakedimau; Franche, Claudine; Ndiku, Sébastien Luyindula

doi:10.9734/ajea/2015/11076

Cited by 2 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results also showed that NaN 3 treatments affected okra germination. NaN 3 -caused seed germination inhibition has also been reported in different plant species [22,23,51,52]. However, NaN 3 stimulated the germination of okra plants [24].…”

Section: Discussionmentioning

confidence: 96%

Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (Abelmoschus esculentus L.)

et al. 2019

View full text Add to dashboard Cite

Waterlogging stress adversely affects crop growth and yield worldwide. Effect of sodium azide priming on waterlogging stress tolerance of okra plants was investigated. The study was conducted as a field experiment using two weeks old plants grown from 0%, 0.02%, and 0.05% sodium azide (NaN 3 )-treated seeds. The waterlogging conditions applied were categorized into control, one week, and two weeks. Different growth and reproductive parameters were investigated. Activity and expression of antioxidant enzymes, root anatomy, and soil chemical analysis were also studied. Results showed that sodium azide priming inhibited germination. The germination percentages recorded were 92.50, 85.00, and 65.00 for 0%, 0.02%, and 0.05% NaN 3 -treated seeds, respectively, nine days after planting. Waterlogging conditions depressed plant height ten weeks after planting. Under waterlogging conditions, NaN 3 promoted plant height and number of leaves formed. NaN 3 also supported the survival of plants and formation of adventitious roots under waterlogging conditions. Waterlogging conditions negatively affected the redox potential, organic C, N, and P concentrations in the soil but enhanced Soil pH, Fe, Mn, Zn, and SO 4 . Under waterlogging conditions, NaN 3 increased the average number of flower buds, flowers, and fruits produced in comparison to control. Moreover, NaN 3 highly stimulated the development of aerenchyma which in turn enhanced the survival of okra plants under waterlogging conditions. NaN 3 priming also enhanced the activities and gene expression level of antioxidant enzymes (ascorbate peroxidase, APX; catalase, CAT) under waterlogging conditions. In conclusion, this study demonstrated that NaN 3 priming could improve waterlogging stress tolerance in okra.

show abstract

Section: Discussionmentioning

confidence: 96%

Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (Abelmoschus esculentus L.)

et al. 2019

View full text Add to dashboard Cite

show abstract

“…This effect of the same extracts on CRPf strain K1 and on isolates of P. falciparum revealed that parasites do not request the same compounds. The difference on phytochemical compounds in P. odontadenius plants was already proved by some works of [26,27] and [28]. The difference on phytochemical composition in M1 and M2 plants could explain the difference on in vitro antiplasmodial activity of plant extracts from irradiated seeds or immersed in SA solutions.…”

Section: Increasing Of In Vitro Antiplasmodial Activitiesmentioning

confidence: 81%

“…These values showed an improvement of in vitro antiplasmodial activity respectively up 810.87% and 338.83% in comparison to the control plant extracts. For the breeding program of P. odontadenius species by SA, values of LD30 and LD50 were respectively 4.76 mM and 10.99 mM [27]. Regarding results, plant extracts from 10 mM in M1 on the clinical isolate of P. falciparum and in M2.2 on CRPf followed values of LD30 and LD50.…”

Section: Increasing Of In Vitro Antiplasmodial Activitiesmentioning

confidence: 88%

“…Plant extracts of Phyllanthus odontadenius from irradiated seeds by gamma rays (Cs-137) or from seeds dipped in Sodium Azide (SA) solutions and cultured in fields were used as biological materials. Physical and chemical Mutagenic techniques were used for gamma irradiation of P. odontadenius seeds or those of soaked seeds in SA resumption respectively in [25,26,27] and [28].…”

Section: Plant Materialsmentioning

confidence: 99%

“…1ml of erythrocytes were mixed with 9 ml of RPMI 1640 containing 25mM HEPES, 25mM sodium. In addition, antimalarial activity assays were performed by microscopic techniques adapting [31] method at the National Institute of Biomedical Research (NIBR) in Kinshasa/ Gombe, DR. Congo [27,32,33,34].…”

Section: Clinical Isolates Of P Falciparummentioning

confidence: 99%

See 2 more Smart Citations

Improvement of Secondary Metabolites from Phyllanthus odontadenius against Malaria by Mutagenesis

Ndiku

Nakweti²,

Sinou

et al. 2018

JPRI

Self Cite

View full text Add to dashboard Cite

Aims: Majority of deaths in children aged under 5 years are due to Plasmodium falciparum malaria. Malaria deaths in children decreased but malaria remains a major killer of children, taking the life of a child every 2 minutes. This study aims to investigate the increasing of the in vitro antiplasmodial activities by mutagenesis techniques using gamma-rays (Cs-137) or sodium azide (NaN 3 ) as mutagens. It will allow the importance of mutagenesis use as tools for improvement of secondary metabolites against malaria parasites using chemical or physical mutagens. Study Design: Laboratory experiment tests : identification of plant material, immersion of seeds in SA (sodium azide) solutions or irradiation by Gamma-rays (Cs-137) of P. odontadenius seeds for improvement of secondary metabolites against malaria parasites, in vitro culture of seeds followed by the in situ culturing of plantlets for obtaining material of study, phytochemical screening of Phyllanthus odontadenius aerial parts to determine the change of compounds in comparison to controls, in vitro antiplasmodial tests for the determination of SA concentrations or those of gammarays doses which killing 50% of malaria parasite populations (IC 50 ).

show abstract

Effects of Sodium Azide (NaN3) on Seeds Germination, Plantlets Growth and In vitro Antimalarial Activities of Phyllanthus odontadenius Müll. Arg.

Cited by 2 publications

References 0 publications

Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (Abelmoschus esculentus L.)

Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (Abelmoschus esculentus L.)

Improvement of Secondary Metabolites from Phyllanthus odontadenius against Malaria by Mutagenesis

Contact Info

Product

Resources

About