Effects of Sodium Azide (NaN3) on Growth of Phyllanthus odontadenius M2 and Evaluation of in vitro Antiplasmodial Activity by Elisa HRP2

Nakweti, Rufin Kikakedimau; Sinou, Véronique; Ndiku, Sébastien Luyindula; Sabot, François; Franche, Claudine

doi:10.9734/ejmp/2017/30181

Cited by 3 publications

(4 citation statements)

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“…This effect of the same extracts on CRPf strain K1 and on isolates of P. falciparum revealed that parasites do not request the same compounds. The difference on phytochemical compounds in P. odontadenius plants was already proved by some works of [26,27] and [28]. The difference on phytochemical composition in M1 and M2 plants could explain the difference on in vitro antiplasmodial activity of plant extracts from irradiated seeds or immersed in SA solutions.…”

Section: Increasing Of In Vitro Antiplasmodial Activitiesmentioning

confidence: 82%

“…Plant extracts of Phyllanthus odontadenius from irradiated seeds by gamma rays (Cs-137) or from seeds dipped in Sodium Azide (SA) solutions and cultured in fields were used as biological materials. Physical and chemical Mutagenic techniques were used for gamma irradiation of P. odontadenius seeds or those of soaked seeds in SA resumption respectively in [25,26,27] and [28].…”

Section: Plant Materialsmentioning

confidence: 99%

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Improvement of Secondary Metabolites from Phyllanthus odontadenius against Malaria by Mutagenesis

Ndiku

Nakweti²,

Sinou

et al. 2018

JPRI

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Aims: Majority of deaths in children aged under 5 years are due to Plasmodium falciparum malaria. Malaria deaths in children decreased but malaria remains a major killer of children, taking the life of a child every 2 minutes. This study aims to investigate the increasing of the in vitro antiplasmodial activities by mutagenesis techniques using gamma-rays (Cs-137) or sodium azide (NaN 3 ) as mutagens. It will allow the importance of mutagenesis use as tools for improvement of secondary metabolites against malaria parasites using chemical or physical mutagens. Study Design: Laboratory experiment tests : identification of plant material, immersion of seeds in SA (sodium azide) solutions or irradiation by Gamma-rays (Cs-137) of P. odontadenius seeds for improvement of secondary metabolites against malaria parasites, in vitro culture of seeds followed by the in situ culturing of plantlets for obtaining material of study, phytochemical screening of Phyllanthus odontadenius aerial parts to determine the change of compounds in comparison to controls, in vitro antiplasmodial tests for the determination of SA concentrations or those of gammarays doses which killing 50% of malaria parasite populations (IC 50 ).

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Section: Increasing Of In Vitro Antiplasmodial Activitiesmentioning

confidence: 82%

Section: Plant Materialsmentioning

confidence: 99%

Improvement of Secondary Metabolites from Phyllanthus odontadenius against Malaria by Mutagenesis

Ndiku

Nakweti²,

Sinou

et al. 2018

JPRI

Self Cite

View full text Add to dashboard Cite

show abstract

“…An atmosphere of 85 % N 2 , 10 % O 2 , and 5 % CO 2 was used to incubate the plates for 72 hours at 37 °C, and then they were frozen at −80 °C. A homemade HRP2‐based ELISA assay revealed parasite growth [59] . The Sunrise TM spectrophotometer (Tecan, Austria) and Magellan TM data analysis software (Tecan, Austria) were used to measure absorbance at 450 nm.…”

Section: Methodsmentioning

confidence: 99%

“…A homemade HRP2-based ELISA assay revealed parasite growth. [59] The Sunrise TM spectrophotometer (Tecan, Austria) and Magellan TM data analysis software (Tecan, Austria) were used to measure absorbance at 450 nm. Non-linear regression analysis was used to calculate the 50 % inhibitory concentrations (IC 50 ) from the dose response relationship fitted by softwareICEstimator 1.2.…”

Section: Determination Of In Vitro Antimalarial Activitymentioning

confidence: 99%

Novel Benzotriazole‐β‐lactam Derivatives as Antimalarial Agents: Design, Synthesis, Biological Evaluation and Molecular Docking Studies

Aye,

Jarrahpour,

Haghighijoo

et al. 2024

Chemistry & Biodiversity

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Many people around the world suffer from malaria, especially in tropical or subtropical regions. While malaria medications have shown success in treating malaria, there is still a problem with resistance to these drugs. Herein, we designed and synthesized some structurally novel benzotriazole‐β‐lactams using 2‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)acetic acid as a key intermediate. To synthesize the target molecules, the ketene‐imine cycloaddition reaction was employed. First, The reaction of 1H‐benzo[d][1,2,3]triazole with 2‐bromoacetic acid in aqueous sodium hydroxide yielded 2‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)acetic acid. Then, the treatment of 2‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)acetic acid with tosyl chloride, triethyl amine, and Schiff base provided new β‐lactams in good to moderate yields..The formation of all cycloadducts was confirmed by elemental analysis, FT‐IR, NMR and mass spectral data. Moreover, X‐ray crystallography was used to determine the relative stereochemistry of 4a compound. The in vitro antimalarial activity test was conducted for each compound against P. falciparum K1. The IC50 values ranged from 5.56 to 25.65 μM. A cytotoxicity profile of the compounds at 200 μM final concentration revealed suitable selectivity of the compounds for malaria treatment. Furthermore, the docking study was carried out for each compound into the P. falciparum dihydrofolate reductase enzyme (PfDHFR) binding site to analyze their possible binding orientation in the active site.

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Synthesis, docking and biological evaluation of some novel [1,2,4] triazolo [5,1-b] quinazoline Schiff base derivatives

Jowkar,

Aboonajmi,

Heiran

et al. 2024

J IRAN CHEM SOC

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Effects of Sodium Azide (NaN3) on Growth of Phyllanthus odontadenius M2 and Evaluation of in vitro Antiplasmodial Activity by Elisa HRP2

Cited by 3 publications

References 0 publications

Improvement of Secondary Metabolites from Phyllanthus odontadenius against Malaria by Mutagenesis

Improvement of Secondary Metabolites from Phyllanthus odontadenius against Malaria by Mutagenesis

Novel Benzotriazole‐β‐lactam Derivatives as Antimalarial Agents: Design, Synthesis, Biological Evaluation and Molecular Docking Studies

Synthesis, docking and biological evaluation of some novel [1,2,4] triazolo [5,1-b] quinazoline Schiff base derivatives

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