Effects of Soaking pH and Extracting Temperature on the Physicochemical Properties of Chicken Skin Gelatin

Kim, Hyun‐Wook; Song, Dong Heon; Choi, Yun‐Sang; Kim, Hack Youn; Hwang, Ko-Eun; Park, Jae Hyun; Kim, Yong Jae; Choi, Jeong-Hoo; Kim, Cheon Jei

doi:10.5851/kosfa.2012.32.3.316

Cited by 29 publications

(26 citation statements)

References 13 publications

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“…The pretreated hides were soaked in distilled water (1:4 w/v) at 65°C (Memmert WNB7-45 type water bath) for 5 h, followed by further soaking at 70°C for 5 h in preparation for the second extraction step. The extracted gelatin was sieved (< 200 mesh) and dried in a cabinet drier at 50-55°C for 48 h until the moisture less than 12% ( Mulyani et al , 2017 ), and the yield was calculated using the following formula ( Kim et al , 2012 ; Ktari et al , 2014 ):…”

Section: Methodsmentioning

confidence: 99%

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Mulyani¹,

Setyabudi²,

Pranoto³

et al. 2017

Korean Journal for Food Science of Animal Resources

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The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M. A completely randomized design and the least significant difference test were used in the experimental design, and all measurements were performed in triplicate. The highest yield (29.17%) was obtained from pretreatment with 0.9 M HCl. The gel strength did not differ significantly (p>0.05) according to acid type (280.26-259.62 g Bloom), and the highest viscosity was obtained from the 0.6 M citric acid pretreatment. All the gelatins contained α- and β-chain components and several degraded peptides (24-66 kDa). The color and Fourier-transform infrared spectrum of the gelatin extracted using 0.9 M HCl were similar to those of commercial bovine skin gelatin. In general, the physicochemical properties of the gelatin complied with the industry standard set by the Gelatin Manufacturers Institute of America, revealing that buffalo hide could serve as a potential alternative source of gelatin.

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Section: Methodsmentioning

confidence: 99%

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Mulyani¹,

Setyabudi²,

Pranoto³

et al. 2017

Korean Journal for Food Science of Animal Resources

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“…Our second objective was to elaborate a biodegradable film using collagen, however following the extraction process (alkali then acid process), the collagen molecule loses its strong mechanical strength compared to the native form [ 30 ]. To overcome such issue, we blended collagen with another natural polymer such as chitosan derived from chitin known as the second most abundant polysaccharide after cellulose.…”

Section: Introductionmentioning

confidence: 99%

Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite

Slimane

Sadok

2018

Marine Drugs

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The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus). The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of α, β and γ chains. As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure. Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film. Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film. Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen. Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.

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“…Kekuatan gel dari gelatin berpengaruh pada aplikasinya pada produk 17 . Perbedaan kondisi ekstraksi akan berpengaruh pada karakteristik gel gelatin 18 . Kekuatan gel sangat tergantung pada ikatan hidrogen antara molekul air dengan kelompok hidroksil bebas dari asam amino, ukuran rantai protein, konsentrasi serta distribusi berat molekul 19,20,21 .…”

Section: Karakteristik Mutu Fisik Gelatin Kaki Ayamunclassified

Pengaruh Penggunaan Pelarut Dan Teknik Ekstraksi Terhadap Mutu Gelatin Kaki Ayam

Miskiyah¹,

Juniawati²,

Kamsiati³

2020

J. Penelit Pascapanen Pertan

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<div><p>Proses produksi gelatin dari bahan baku alternatif diperlukan untuk mengatasi kebutuhan gelatin halal. Saat ini, terdapat batasan penggunaan gelatin, terkait dengan aspek kehalalannya. Eksplorasi proses produksi gelatin dari limbah pemotongan ayam memerlukan berbagai modifikasi agar memenuhi standar mutu gelatin yang telah ditetapkan oleh Standar Nasional Indonesia (SNI). Tujuan penelitian ini adalah mengetahui sifat fisikokimia gelatin yang dihasilkan dari kaki ayam dan karakteristiknya dari proses modifikasi gelatin. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap dengan empat ulangan. Perlakuan yang digunakan dalam penelitian adalah bahan pelarut (A) (A1: HCl 1%,, A2: NaOH 1%,; A3: CH3COOH 1%) dan metode ekstraksi (B) (B1: pemanasan menggunakan water bath dan B2: menggunakan panci bertekanan/presto). Hasil penelitian menunjukkan bahwa kaki ayam memiliki kandungan protein yang cukup tinggi, sehingga berpotensi digunakan sebagai bahan baku untuk produksi gelatin. Perlakuan terbaik adalah 2 hari ekstraksi menggunakan pelarut CH3COOH 1%, dengan proses ekstraksi menggunakan water bath selama 1 jam. Rendemen gelatin 6,75 ± 1,89%; kadar air 10,41 ± 0,63%; kadar abu 3,90 ± 0,43%; kandungan lemak 9,68 ± 1,21%; kandungan protein 77,21 ± 0,58%; pH 5,62 ± 0,13; bersifat larut; viskositas 6,67 ± 1,53 cPs; kekuatan gel 164,75 ± 3,40 g bloom; kandungan residu Cu 4,52 ± 0,71 ppm; Zn 9,92 ± 0,22 ppm; As 0,000 ± 0,00 ppm; dan Sulfit 5.56 ± 0,30 ppm. Proses produksi gelatin dengan menggunakan metode asam dan proses ekstraksi dengan <em>water bath </em>belum menghasilkan gelatin yang sesuai dengan standar mutu gelatin (SNI), sehingga perlu perbaikan dalam metode ekstraksi.</p></div><div> </div>Production process of gelatin from alternatif material is needed to overcome the supply of halal gelatin. There are several obstacles to the use of gelatin, one of this related to religious aspects, that is prohibition of consuming ingredients from pigs. Explorations of production process of gelatin from waste of chicken slaughtering house need some modiifications, so can meet to the Indonesian Standard Regulation (SNI). The purpose of this research was to know the physicochemical properties of gelatin produced from chicken feet, and their characteristics from modification process of gelatin. The results showed that chicken feet has a high enough protein content, so it was potential to be used as a raw material for gelatin production. The best treatment was 2 days exraction using CH3COOH 1% solvent, followed by heating using waterbath for 1 hour. Yield of gelatin 6.75 ± 1.89%; water content 10,41 ± 0,63%; ash content 3,90 ± 0,43%; fat content 9.68 ± 1.21%; protein content 77,21 ± 0,58%; pH 5.62 ± 0.13; solubility was soluble; viscosity 6.67 ± 1.53 cPs; gel strength 164.75 ± 3.40 bloom; Cu residue content of 4.52 ± 0.71 ppm; Zn 9,92 ± 0,22 ppm; As 0,000 ± 0.00 ppm; Sulfite 5.56 ± 0.30 ppm. However, it is need improvement to reduce the fat content in gelatin product

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Effects of Soaking pH and Extracting Temperature on the Physicochemical Properties of Chicken Skin Gelatin

Cited by 29 publications

References 13 publications

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite

Pengaruh Penggunaan Pelarut Dan Teknik Ekstraksi Terhadap Mutu Gelatin Kaki Ayam

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