Effects of small intestinal submucosa content on the adhesion and proliferation of retinal pigment epithelial cells on SIS-PLGA films

Abstract

The retinal pigment epithelium (RPE) plays a critical role in the maintenance of the normal functions of the retina, particularly the photoreceptors. RPE dysfunction, vision loss and degeneration have been implicated as the cause of many retinal diseases, including retinitis pigmentosa and age-related macular degeneration (AMD). To overcome such disorders, tissue engineering could offer useful strategies, using biodegradable polymeric films to replace diseased or lost RPE. Synthetic/natural hybrid films have been studied as a temporary substrate for growing RPEs in biological implantations. In this study, we prepared small intestinal submucosa (SIS)-poly(lactic-co-glycolic) (PLGA) hybrid films and seeded human RPE cells (ARPE-19 cells) onto the film surface. We investigated the film suitability for RPE cell proliferation by MTT assay. The morphology of cellular adhesion on the film was confirmed by scanning electron microscopy (SEM). Reverse transcription-polymerase chain reaction (RT-PCR) and 3-amino-9-ethylcarbazole (AEC) staining were performed to examine mRNA expression and to compare cell proliferation on the films, using cytokeratin as a marker of RPE. Conclusively, we confirmed the higher cell survival rate and much stronger phenotype expression of RPEs on SIS-PLGA films compared to pure PLGA films. These results demonstrated the potential application of SIS-PLGA films in tissue-engineering strategies. Copyright © 2014 John Wiley & Sons, Ltd.

Surface modification of small intestine submucosa in tissue engineering

“…The flaky SIS was first used in the study of repairing blood vessels, and vascular endothelial cells were planted on SIS to construct regenerative tissue engineering blood vessels [ 45 , 46 ]. In addition, SIS is used for bladder reconstruction and repairing bone, cartilage and tendon and other aspects [ 18 , 21 , 47 ].…”

“…The porcine SIS piece was pulverized, suspended in HCl solution with porcine pepsin, stirred at room temperature, adjusted to pH 7.4, lyophilized and pulverized to obtain an SIS powder. The SIS powder was dissolved in PBS at 20% w/v and shaped into a gel of any shape overnight at 37 °C [ 41 , 47 , 50 ]. For example, SIS hydrogel placed on the bottom of the culture dish forms a flat shape [ 41 , 51 ], or put in a small bottle forms a thicker cylindrical shape.…”

“…In the practical applications, some attempts were applied on SIS for enhancing its function. For example, the SIS is made into a tubule with crosslinking of various biologically active factors and transplanted into animal to substitute carotid arteries [ 23 , 47 ]. SIS was loaded with cardiomyocytes and then was transplanted to the outer surface of infarcted myocardium in mice to repair heart function [ 16 ].…”

Progress in Silk Fibroin Based Composite Scaffold/Hydrogel: Silk Fibroin/PEG Hydrogel for the RPE Regeneration a Promising Biomaterial for Clinical Application

“…Natural polymers that can be used as a substrates for RPE regeneration including collagen, alginate, hyaluronic acid, gellan gum, gelatin, and silk fibroin (SF) and synthetic polymers including PLGA, PCL, and PEG (Lee et al, 2017;Wang and Han, 2017;Park et al, 2018;Kim et al, 2019;Shin et al, 2019). As a carrier that delivers RPE cells, it should have good cell adhesion, survival rate, invasiveness and biocompatible properties that helps in binding to other tissues of the retina.…”

LYTAK1 attenuates proliferation of retinal pigment epithelial cells through TGF‑β‑mediated epithelial‑mesenchymal transition via the ERK/AKT signaling pathway

“…RPE has a key role in maintaining the normal function of the retina, particularly the photoreceptor in the optic nerve system ( 3 , 4 ). RPE dysfunction, loss of vision and degradation are associated with various diseases of the retina, such as retinitis pigment and age-related macular degeneration (AMD) ( 5 ). In recent years, increasing reports have investigated the role of RPE in cell pyroptosis, death, inflammasome and lipofuscin phototoxicity ( 2 , 6 ).…”