Effects of Saturated and Unsaturated Fatty Acids on Blood Platelet Aggregation in vitro.

Mahadevan, V.; Singh, Harminder; Lundberg, W. O.

doi:10.3181/00379727-121-30702

Cited by 41 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, at a given concentration, much more of the saturated fatty acids, palmitate and stearate, associated with the platelets as FFA. These acids in high concentrations are considerably more potent than the unsaturated acids in inducing aggregation (9,10). Moreover, as the FFA-albumin molar ratio was raised, the percentage increase in uptake as platelet FFA increased markedly whereas the percentage increases in FFA oxidation and esterification were much less pronounced.…”

Section: Discussionmentioning

confidence: 95%

Utilization of long-chain free fatty acids by human platelets

Spector¹,

Hoak²,

Warner³

et al. 1970

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T There was a rapid net uptake of free fatty acid (FFA) by human platelets when long-chain FFA, bound to human serum albumin, were incubated with platelet suspensions. Results from experiments in which both palmitate and albumin were labeled indicated that the fatty acid dissociated from the protein during uptake. Much of the FFA taken up by the platelet in short-term incubations remained in unesterified form, i.e., it was recovered as platelet FFA. As the incubation continued, increasing amounts of FFA were oxidized to CO2 and incorporated into platelet lipid esters, particularly lecithin. Essentially all of the fatty acid that was incorporated into the platelet FFA fraction was released rapidly from the cells when they were exposed to a medium containing FFA-free albumin. The magnitude of uptake into the platelet FFA fraction was similiar at 00 and 370C. Likewise, the rate and magnitude of FFA release from the platelet were similar at 00 and 37°C. Therefore, it is likely that both FFA uptake and FFA release occur by energy-independent mechanisms. The major effect of increasing the FFA concentration of the incubation medium was increased fatty acid uptake into the platelet FFA fraction. Similar results occurred when platelets were incubated in human plasma containing increasing amounts of added palmitate. At a given extracellular FFA concentration, considerably more of the saturated fatty acids, palmitate and stearate, were taken up as platelet FFA (4,5). In contrast, more linoleate than stearate was incorporated into lipid esters in both of these cells (4, 5).Human platelets, like most other mammalian cells, take up and metabolize long-chain FFA (6-8). Palmitic acid has been employed in most studies of FFA metabolism in platelets (6, 7), and except for incorporated into phospholipids (8), little is known about the way in which the other commonly occurring long-chain fatty acids are utilized. This question is of particular relevance for platelets because it has been reported that saturated FFA are much more effective than unsaturated FFA in causing platelet aggregation (9, 10). Such effects may result from differences in the way that platelets take up or metabolize saturated and unsaturated FFA. In order to examine this point, we compared the utilization of palmitic, stearic, oleic, and linoleic acids by suspensions of human platelets. The major difference that we observed was the extent to which each of these fatty acids was incorporated in unesterified form by the platelet. in order to obtain the platelet pellet. After removing the plasma, the platelets were resuspended in a solution containing 0.123 M NaCl, 0.0045 M KCl, and 0.015 m Tris buffer adjusted to pH 7.4 with 1 N HCL. The platelet count of each suspension also was determined, and enough Trisbuffered salt solution was added so that 1 ml of the suspension contained 109 platelets. Microscopic examination of the platelet suspension revealed only trace contamination with other cellular components of the blood. White blood cells were the ...

show abstract

Section: Discussionmentioning

confidence: 95%

Utilization of long-chain free fatty acids by human platelets

Spector¹,

Hoak²,

Warner³

et al. 1970

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Other fatty acids cause in vitro aggregation, but the effect develops more slowly and often only in the absence of serum proteins [8,11]. Platelets incubated with NaA released rabbit aorta contracting substances, but no release occurred with linoleic, linolenic, and stearic acid [23].…”

Section: Discussionmentioning

confidence: 99%

Circulating Platelet Aggregates and Thrombocytopenia Induced by Intravenous Infusions of Arachidonic and Lauric Acids in Guinea Pigs

Rafflenbeul

Zbinden²

1977

Acta Haematol

View full text Add to dashboard Cite

Slow intravenous infusion of Na laurate (NaL) into guinea pigs caused a rapid appearance of platelet aggregates in the arterial blood and a precipitous fall in platelet counts. During the infusion of Na arachidonate (NaA) thrombocytopenia developed slowly, and few and smaller platelet aggregates appeared in the arterial blood. Considerably more guinea pigs died during or after the NaA infusion than after administration of NaL. The possibility that arachidonic acid and other long-chain fatty acids may play a role in the development of thrombosis and thromboembolism is discussed.

show abstract

“…Der Zu satz von Natrium-Oleat zu Blut in Konzentrationen bis 120 mg % ver ändert den pH des Blutes bzw. Plasmas nicht [12]; die Wirkungen auf Erythrozyten [13,12,10] [18] wurde nachgewiesen, dass Natrium-Oleat sowohl auf gewaschene Thrombozyten als auch auf Thrombozyten in plättchcnrcichcm Plasma aggregationsfördernd wirkt. Relativ niedrige Natrium-Oleat-Konzentrationen (bis 60 mg%) aggregierten nach weiteren Feststellungen jedoch lediglich gewaschene Thrombozyten, nicht aber Blutplättchen in Plasma [16].…”

Section: Diskussionunclassified